Restriction map for
pF1KE0013
(Linear) (Six-base) MAPSORT of: KIEE0013.seq Check: 8822 from: 1 to: 2169
KIEE0013.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 2111 2169
Size: 2111 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 403 982 1366 2169
Size: 41 362 579 384 803
Fragments arranged by size:
803 579 384 362 41
AflIII A'CryG_T
Cuts at: 0 126 2169
Size: 126 2043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1612 2169
Size: 1612 557
ApoI r'AATT_y
Cuts at: 0 229 1138 1153 1176 1672 2169
Size: 229 909 15 23 496 497
Fragments arranged by size:
909 497 496 229 23 15
AseI AT'TA_AT
Cuts at: 0 1049 1331 1645 1657 2169
Size: 1049 282 314 12 512
Fragments arranged by size:
1049 512 314 282 12
BanI G'GyrC_C
Cuts at: 0 324 1218 2169
Size: 324 894 951
Fragments arranged by size:
951 894 324
BanII G_rGCy'C
Cuts at: 0 120 2169
Size: 120 2049
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 391 1247 2169
Size: 47 344 856 922
Fragments arranged by size:
922 856 344 47
BbvCI CC'TCA_GC
Cuts at: 0 1877 2169
Size: 1877 292
BciVI GTATCCnnnnn_n'
Cuts at: 0 428 2169
Size: 428 1741
Bme1580I G_kGCm'C
Cuts at: 0 663 1130 2169
Size: 663 467 1039
Fragments arranged by size:
1039 663 467
BmrI ACTGGGnnnn_n'
Cuts at: 0 666 2169
Size: 666 1503
BmtI G_CTAG'C
Cuts at: 0 437 574 2169
Size: 437 137 1595
Fragments arranged by size:
1595 437 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 2169
Size: 1709 460
Bpu10I CC'TnA_GC
Cuts at: 0 1877 2169
Size: 1877 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1211 1816 2169
Size: 1211 605 353
Fragments arranged by size:
1211 605 353
BsaI GGTCTCn'nnnn_
Cuts at: 0 1618 2169
Size: 1618 551
BsaAI yAC'GTr
Cuts at: 0 1407 2169
Size: 1407 762
BsaBI GATnn'nnATC
Cuts at: 0 1823 2169
Size: 1823 346
BsaWI w'CCGG_w
Cuts at: 0 420 2169
Size: 420 1749
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1186 2169
Size: 1186 983
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 674 2169
Size: 421 253 1495
Fragments arranged by size:
1495 421 253
BsiHKAI G_wGCw'C
Cuts at: 0 120 2169
Size: 120 2049
BsmI GAATG_Cn'
Cuts at: 0 584 2169
Size: 584 1585
Bsp1286I G_dGCh'C
Cuts at: 0 120 663 1130 2169
Size: 120 543 467 1039
Fragments arranged by size:
1039 543 467 120
BspEI T'CCGG_A
Cuts at: 0 420 2169
Size: 420 1749
BspHI T'CATG_A
Cuts at: 0 2061 2169
Size: 2061 108
BsrDI GCAATG_nn'
Cuts at: 0 468 1445 1950 1991 2169
Size: 468 977 505 41 178
Fragments arranged by size:
977 505 468 178 41
BsrGI T'GTAC_A
Cuts at: 0 1557 2169
Size: 1557 612
BstYI r'GATC_y
Cuts at: 0 2122 2169
Size: 2122 47
BstZ17I GTA'TAC
Cuts at: 0 2112 2169
Size: 2112 57
BtsI GCAGTG_nn'
Cuts at: 0 404 2169
Size: 404 1765
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1993 2028 2169
Size: 1993 35 141
Fragments arranged by size:
1993 141 35
DraI TTT'AAA
Cuts at: 0 707 2169
Size: 707 1462
EaeI y'GGCC_r
Cuts at: 0 1592 2001 2169
Size: 1592 409 168
Fragments arranged by size:
1592 409 168
EarI CTCTTCn'nnn_
Cuts at: 0 77 2169
Size: 77 2092
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2169
Size: 19 2150
EcoICRI GAG'CTC
Cuts at: 0 118 2169
Size: 118 2051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 403 982 1366 1709 2169
Size: 41 362 579 384 343 460
Fragments arranged by size:
579 460 384 362 343 41
EcoO109I rG'GnC_Cy
Cuts at: 0 1954 2169
Size: 1954 215
EcoRI G'AATT_C
Cuts at: 0 1672 2169
Size: 1672 497
EcoRV GAT'ATC
Cuts at: 0 2156 2169
Size: 2156 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 2169
Size: 183 32 1954
Fragments arranged by size:
1954 183 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 2169
Size: 266 1903
MscI TGG'CCA
Cuts at: 0 2003 2169
Size: 2003 166
MslI CAynn'nnrTG
Cuts at: 0 775 1279 1594 2169
Size: 775 504 315 575
Fragments arranged by size:
775 575 504 315
MspA1I CmG'CkG
Cuts at: 0 175 1002 1735 2169
Size: 175 827 733 434
Fragments arranged by size:
827 733 434 175
NdeI CA'TA_TG
Cuts at: 0 2088 2169
Size: 2088 81
NheI G'CTAG_C
Cuts at: 0 433 570 2169
Size: 433 137 1599
Fragments arranged by size:
1599 433 137
NspI r_CATG'y
Cuts at: 0 130 680 1134 1488 2169
Size: 130 550 454 354 681
Fragments arranged by size:
681 550 454 354 130
PciI A'CATG_T
Cuts at: 0 126 2169
Size: 126 2043
PpuMI rG'GwC_Cy
Cuts at: 0 1954 2169
Size: 1954 215
PsiI TTA'TAA
Cuts at: 0 308 884 1093 1889 2169
Size: 308 576 209 796 280
Fragments arranged by size:
796 576 308 280 209
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2110 2142 2169
Size: 2110 32 27
Fragments arranged by size:
2110 32 27
PstI C_TGCA'G
Cuts at: 0 1217 2169
Size: 1217 952
PvuII CAG'CTG
Cuts at: 0 1002 1735 2169
Size: 1002 733 434
Fragments arranged by size:
1002 733 434
SacI G_AGCT'C
Cuts at: 0 120 2169
Size: 120 2049
SanDI GG'GwC_CC
Cuts at: 0 1954 2169
Size: 1954 215
SbfI CC_TGCA'GG
Cuts at: 0 1217 2169
Size: 1217 952
SfcI C'TryA_G
Cuts at: 0 23 922 1213 1666 1968 2169
Size: 23 899 291 453 302 201
Fragments arranged by size:
899 453 302 291 201 23
SmlI C'TyrA_G
Cuts at: 0 1226 1831 2169
Size: 1226 605 338
Fragments arranged by size:
1226 605 338
SpeI A'CTAG_T
Cuts at: 0 181 2169
Size: 181 1988
SphI G_CATG'C
Cuts at: 0 680 2169
Size: 680 1489
SspI AAT'ATT
Cuts at: 0 1089 1568 2169
Size: 1089 479 601
Fragments arranged by size:
1089 601 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1577 1753 2169
Size: 1577 176 416
Fragments arranged by size:
1577 416 176
TatI w'GTAC_w
Cuts at: 0 1066 1460 1557 2169
Size: 1066 394 97 612
Fragments arranged by size:
1066 612 394 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1778 1810 2169
Size: 1778 32 359
Fragments arranged by size:
1778 359 32
Tth111I GACn'n_nGTC
Cuts at: 0 1251 2169
Size: 1251 918
XmnI GAAnn'nnTTC
Cuts at: 0 2098 2169
Size: 2098 71
Enzymes that do cut:
AccI AcuI AflIII AlfI ApoI AseI BanI BanII
BbsI BbvCI BciVI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrDI BsrGI BstYI BstZ17I
BtsI CspCI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV Hin4I MmeI MscI MslI MspA1I
NdeI NheI NspI PciI PpuMI PsiI PsrI PstI
PvuII SacI SanDI SbfI SfcI SmlI SpeI SphI
SspI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BplI BsaHI BsaXI BseYI BsiEI
BsiWI BsmBI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI Bsu36I BtgI BtgZI ClaI DraIII
DrdI EagI EcoNI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PvuI RsrII SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI StuI StyI
SwaI XbaI XcmI XhoI XmaI ZraI