Restriction map for
pF1KE0009
(Linear) (Six-base) MAPSORT of: KIEE0009.seq Check: 2378 from: 1 to: 1929
KIEE0009.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 1061 1129 1195 1929
Size: 467 594 68 66 734
Fragments arranged by size:
734 594 467 68 66
AlwNI CAG_nnn'CTG
Cuts at: 0 1879 1929
Size: 1879 50
ApoI r'AATT_y
Cuts at: 0 71 161 1035 1357 1439 1929
Size: 71 90 874 322 82 490
Fragments arranged by size:
874 490 322 90 82 71
AvrII C'CTAG_G
Cuts at: 0 76 1929
Size: 76 1853
BamHI G'GATC_C
Cuts at: 0 1643 1929
Size: 1643 286
BanI G'GyrC_C
Cuts at: 0 425 1929
Size: 425 1504
BanII G_rGCy'C
Cuts at: 0 359 983 1929
Size: 359 624 946
Fragments arranged by size:
946 624 359
BbsI GAAGACnn'nnnn_
Cuts at: 0 1372 1929
Size: 1372 557
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 978 1012 1929
Size: 978 34 917
Fragments arranged by size:
978 917 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1805 1929
Size: 1805 124
BglII A'GATC_T
Cuts at: 0 95 1929
Size: 95 1834
Bme1580I G_kGCm'C
Cuts at: 0 430 1929
Size: 430 1499
BmgBI CAC'GTC
Cuts at: 0 1581 1929
Size: 1581 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 716 997 1929
Size: 281 435 281 932
Fragments arranged by size:
932 435 281 281
Bpu10I CC'TnA_GC
Cuts at: 0 353 1929
Size: 353 1576
BsaI GGTCTCn'nnnn_
Cuts at: 0 613 1929
Size: 613 1316
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 1720 1750 1929
Size: 220 30 1470 30 179
Fragments arranged by size:
1470 220 179 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 515 589 686 689 715 720 757 760
Size: 515 74 97 3 26 5 37 3
Cuts at: 760 763 772 775 778 787 796 805 814
Size: 3 9 3 3 9 9 9 9
Cuts at: 814 830 848 1929
Size: 16 18 1081
Fragments arranged by size:
1081 515 97 74 37 26 18 16
9 9 9 9 9 5 3 3
3 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 1594 1929
Size: 345 1249 335
Fragments arranged by size:
1249 345 335
BsiHKAI G_wGCw'C
Cuts at: 0 895 983 1929
Size: 895 88 946
Fragments arranged by size:
946 895 88
BsmI GAATG_Cn'
Cuts at: 0 460 836 1096 1929
Size: 460 376 260 833
Fragments arranged by size:
833 460 376 260
Bsp1286I G_dGCh'C
Cuts at: 0 359 430 895 983 1929
Size: 359 71 465 88 946
Fragments arranged by size:
946 465 359 88 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 1929
Size: 376 1553
BsrGI T'GTAC_A
Cuts at: 0 329 1929
Size: 329 1600
BstAPI GCAn_nnn'nTGC
Cuts at: 0 368 1929
Size: 368 1561
BstEII G'GTnAC_C
Cuts at: 0 1254 1929
Size: 1254 675
BstYI r'GATC_y
Cuts at: 0 95 1643 1929
Size: 95 1548 286
Fragments arranged by size:
1548 286 95
Bsu36I CC'TnA_GG
Cuts at: 0 527 1929
Size: 527 1402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 981 1929
Size: 981 948
BtsI GCAGTG_nn'
Cuts at: 0 871 1929
Size: 871 1058
ClaI AT'CG_AT
Cuts at: 0 1220 1929
Size: 1220 709
EaeI y'GGCC_r
Cuts at: 0 1662 1929
Size: 1662 267
EarI CTCTTCn'nnn_
Cuts at: 0 381 1318 1929
Size: 381 937 611
Fragments arranged by size:
937 611 381
EcoICRI GAG'CTC
Cuts at: 0 981 1929
Size: 981 948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 467 716 997 1061 1129 1195 1929
Size: 281 186 249 281 64 68 66 734
Fragments arranged by size:
734 281 281 249 186 68 66 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 863 1929
Size: 863 1066
EcoO109I rG'GnC_Cy
Cuts at: 0 804 1929
Size: 804 1125
EcoRI G'AATT_C
Cuts at: 0 161 1929
Size: 161 1768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 580 1929
Size: 580 1349
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 957 989 1720 1752 1780 1812 1929
Size: 957 32 731 32 28 32 117
Fragments arranged by size:
957 731 117 32 32 32 28
HincII GTy'rAC
Cuts at: 0 210 1929
Size: 210 1719
HindIII A'AGCT_T
Cuts at: 0 1102 1929
Size: 1102 827
HpaI GTT'AAC
Cuts at: 0 210 1929
Size: 210 1719
MfeI C'AATT_G
Cuts at: 0 1165 1929
Size: 1165 764
MscI TGG'CCA
Cuts at: 0 1664 1929
Size: 1664 265
MslI CAynn'nnrTG
Cuts at: 0 325 1929
Size: 325 1604
MspA1I CmG'CkG
Cuts at: 0 917 951 1929
Size: 917 34 978
Fragments arranged by size:
978 917 34
PfoI T'CCnGG_A
Cuts at: 0 258 1929
Size: 258 1671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 1929
Size: 105 32 1792
Fragments arranged by size:
1792 105 32
PpuMI rG'GwC_Cy
Cuts at: 0 804 1929
Size: 804 1125
PsiI TTA'TAA
Cuts at: 0 278 1487 1929
Size: 278 1209 442
Fragments arranged by size:
1209 442 278
PvuII CAG'CTG
Cuts at: 0 917 1929
Size: 917 1012
SacI G_AGCT'C
Cuts at: 0 983 1929
Size: 983 946
SapI GCTCTTCn'nnn_
Cuts at: 0 1318 1929
Size: 1318 611
ScaI AGT'ACT
Cuts at: 0 1619 1929
Size: 1619 310
SfcI C'TryA_G
Cuts at: 0 903 1929
Size: 903 1026
StuI AGG'CCT
Cuts at: 0 693 1679 1929
Size: 693 986 250
Fragments arranged by size:
986 693 250
StyI C'CwwG_G
Cuts at: 0 76 732 1623 1929
Size: 76 656 891 306
Fragments arranged by size:
891 656 306 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 557 1929
Size: 557 1372
TatI w'GTAC_w
Cuts at: 0 329 1476 1617 1929
Size: 329 1147 141 312
Fragments arranged by size:
1147 329 312 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1570 1602 1929
Size: 1570 32 327
Fragments arranged by size:
1570 327 32
Tth111I GACn'n_nGTC
Cuts at: 0 1732 1929
Size: 1732 197
XbaI T'CTAG_A
Cuts at: 0 631 1542 1884 1929
Size: 631 911 342 45
Fragments arranged by size:
911 631 342 45
XmnI GAAnn'nnTTC
Cuts at: 0 71 563 1772 1929
Size: 71 492 1209 157
Fragments arranged by size:
1209 492 157 71
Enzymes that do cut:
AcuI AlwNI ApoI AvrII BamHI BanI BanII BbsI
BcgI BciVI BglII Bme1580I BmgBI BpmI Bpu10I BsaI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI
BstAPI BstEII BstYI Bsu36I BtgZI BtsI ClaI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII HindIII HpaI MfeI MscI MslI MspA1I PfoI
PpiI PpuMI PsiI PvuII SacI SapI ScaI SfcI
StuI StyI TaqII TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BbeI BbvCI BclI BfrBI
BglI BlpI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstXI BstZ17I
BtgI CspCI DraI DraIII DrdI EagI EciI EcoRV
FseI FspI FspAI HaeII KasI KpnI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
XcmI XhoI XmaI ZraI