Restriction map for
pF1KE0008
(Linear) (Six-base) MAPSORT of: KIEE0008.seq Check: 7163 from: 1 to: 2091
KIEE0008.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1534 2091
Size: 1534 557
AccI GT'mk_AC
Cuts at: 0 1330 2091
Size: 1330 761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 2091
Size: 77 2014
AflIII A'CryG_T
Cuts at: 0 1305 2091
Size: 1305 786
AhdI GACnn_n'nnGTC
Cuts at: 0 796 2091
Size: 796 1295
AlwNI CAG_nnn'CTG
Cuts at: 0 745 2091
Size: 745 1346
ApoI r'AATT_y
Cuts at: 0 180 425 472 779 905 1139 1320 1641
Size: 180 245 47 307 126 234 181 321
Cuts at: 1641 2091
Size: 450
Fragments arranged by size:
450 321 307 245 234 181 180 126
47
AseI AT'TA_AT
Cuts at: 0 1676 2091
Size: 1676 415
BanII G_rGCy'C
Cuts at: 0 1196 1539 2091
Size: 1196 343 552
Fragments arranged by size:
1196 552 343
BbsI GAAGACnn'nnnn_
Cuts at: 0 795 1081 2091
Size: 795 286 1010
Fragments arranged by size:
1010 795 286
BfrBI ATG'CAT
Cuts at: 0 87 2091
Size: 87 2004
Bme1580I G_kGCm'C
Cuts at: 0 1514 2091
Size: 1514 577
BmrI ACTGGGnnnn_n'
Cuts at: 0 1782 2091
Size: 1782 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 2091
Size: 499 1592
Bpu10I CC'TnA_GC
Cuts at: 0 147 2091
Size: 147 1944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 2091
Size: 1167 924
BsaI GGTCTCn'nnnn_
Cuts at: 0 1375 2091
Size: 1375 716
BsaAI yAC'GTr
Cuts at: 0 159 1490 2091
Size: 159 1331 601
Fragments arranged by size:
1331 601 159
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1777 1807 2091
Size: 1777 30 284
Fragments arranged by size:
1777 284 30
BsiHKAI G_wGCw'C
Cuts at: 0 412 2091
Size: 412 1679
BsmI GAATG_Cn'
Cuts at: 0 87 844 1431 2091
Size: 87 757 587 660
Fragments arranged by size:
757 660 587 87
Bsp1286I G_dGCh'C
Cuts at: 0 412 1196 1514 1539 2091
Size: 412 784 318 25 552
Fragments arranged by size:
784 552 412 318 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1534 2091
Size: 1534 557
BsrDI GCAATG_nn'
Cuts at: 0 53 497 2091
Size: 53 444 1594
Fragments arranged by size:
1594 444 53
BsrGI T'GTAC_A
Cuts at: 0 1308 2091
Size: 1308 783
BstYI r'GATC_y
Cuts at: 0 1991 2091
Size: 1991 100
BtgI C'CryG_G
Cuts at: 0 771 1181 1518 2091
Size: 771 410 337 573
Fragments arranged by size:
771 573 410 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1913 2091
Size: 1913 178
BtsI GCAGTG_nn'
Cuts at: 0 1288 1700 2091
Size: 1288 412 391
Fragments arranged by size:
1288 412 391
DraI TTT'AAA
Cuts at: 0 342 2091
Size: 342 1749
EaeI y'GGCC_r
Cuts at: 0 1904 2091
Size: 1904 187
EarI CTCTTCn'nnn_
Cuts at: 0 1201 2091
Size: 1201 890
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 499 2091
Size: 77 422 1592
Fragments arranged by size:
1592 422 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 561 593 690 722 782 814
Size: 319 32 210 32 97 32 60 32
Cuts at: 814 906 938 2091
Size: 92 32 1153
Fragments arranged by size:
1153 319 210 97 92 60 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1445 2091
Size: 1445 646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1215 2091
Size: 468 747 876
Fragments arranged by size:
876 747 468
MscI TGG'CCA
Cuts at: 0 1906 2091
Size: 1906 185
MslI CAynn'nnrTG
Cuts at: 0 511 1517 2091
Size: 511 1006 574
Fragments arranged by size:
1006 574 511
NcoI C'CATG_G
Cuts at: 0 1181 1518 2091
Size: 1181 337 573
Fragments arranged by size:
1181 573 337
NdeI CA'TA_TG
Cuts at: 0 1373 2091
Size: 1373 718
NsiI A_TGCA'T
Cuts at: 0 89 2091
Size: 89 2002
NspI r_CATG'y
Cuts at: 0 87 1155 1309 1341 2091
Size: 87 1068 154 32 750
Fragments arranged by size:
1068 750 154 87 32
PciI A'CATG_T
Cuts at: 0 1305 2091
Size: 1305 786
PflMI CCAn_nnn'nTGG
Cuts at: 0 290 1135 1206 1913 2091
Size: 290 845 71 707 178
Fragments arranged by size:
845 707 290 178 71
PstI C_TGCA'G
Cuts at: 0 1270 2091
Size: 1270 821
SapI GCTCTTCn'nnn_
Cuts at: 0 1201 2091
Size: 1201 890
SfcI C'TryA_G
Cuts at: 0 1266 2091
Size: 1266 825
SmlI C'TyrA_G
Cuts at: 0 1146 2091
Size: 1146 945
SnaBI TAC'GTA
Cuts at: 0 159 1490 2091
Size: 159 1331 601
Fragments arranged by size:
1331 601 159
SphI G_CATG'C
Cuts at: 0 1341 2091
Size: 1341 750
SspI AAT'ATT
Cuts at: 0 373 2091
Size: 373 1718
StyI C'CwwG_G
Cuts at: 0 1181 1518 1651 2091
Size: 1181 337 133 440
Fragments arranged by size:
1181 440 337 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1531 1644 2091
Size: 1531 113 447
Fragments arranged by size:
1531 447 113
TatI w'GTAC_w
Cuts at: 0 1308 2091
Size: 1308 783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1817 1849 1886 1918 2091
Size: 1817 32 37 32 173
Fragments arranged by size:
1817 173 37 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 592 792 2091
Size: 592 200 1299
Fragments arranged by size:
1299 592 200
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlwNI ApoI AseI
BanII BbsI BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrGI BstYI BtgI BtgZI BtsI DraI EaeI EarI
Eco57MI Hin4I HindIII MmeI MscI MslI NcoI NdeI
NsiI NspI PciI PflMI PstI SapI SfcI SmlI
SnaBI SphI SspI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmtI BplI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NgoMIV NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SrfI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI