Restriction map for
pF1KE0006
(Linear) (Six-base) MAPSORT of: KIEE0006.seq Check: 7759 from: 1 to: 1563
KIEE0006.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 71 1451 1563
Size: 71 1380 112
Fragments arranged by size:
1380 112 71
AfeI AGC'GCT
Cuts at: 0 216 1563
Size: 216 1347
AleI CACnn'nnGTG
Cuts at: 0 1102 1563
Size: 1102 461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 593 821 1563
Size: 593 228 742
Fragments arranged by size:
742 593 228
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 709 741 818 850 1563
Size: 709 32 77 32 713
Fragments arranged by size:
713 709 77 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1563
Size: 939 624
ApoI r'AATT_y
Cuts at: 0 106 157 1141 1245 1563
Size: 106 51 984 104 318
Fragments arranged by size:
984 318 106 104 51
AseI AT'TA_AT
Cuts at: 0 464 1563
Size: 464 1099
AvaI C'yCGr_G
Cuts at: 0 654 1563
Size: 654 909
BamHI G'GATC_C
Cuts at: 0 833 1563
Size: 833 730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1048 1082 1563
Size: 1048 34 481
Fragments arranged by size:
1048 481 34
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 233 1563
Size: 233 1330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1563
Size: 61 1502
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 991 1563
Size: 697 294 572
Fragments arranged by size:
697 572 294
BsaI GGTCTCn'nnnn_
Cuts at: 0 584 666 1563
Size: 584 82 897
Fragments arranged by size:
897 584 82
BsaBI GATnn'nnATC
Cuts at: 0 1274 1563
Size: 1274 289
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 986 1016 1563
Size: 986 30 547
Fragments arranged by size:
986 547 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 225 994 1563
Size: 58 167 769 569
Fragments arranged by size:
769 569 167 58
BseYI C'CCAG_C
Cuts at: 0 194 566 1563
Size: 194 372 997
Fragments arranged by size:
997 372 194
BsrDI GCAATG_nn'
Cuts at: 0 654 1184 1563
Size: 654 530 379
Fragments arranged by size:
654 530 379
BsrGI T'GTAC_A
Cuts at: 0 1416 1563
Size: 1416 147
BstYI r'GATC_y
Cuts at: 0 774 833 998 1563
Size: 774 59 165 565
Fragments arranged by size:
774 565 165 59
BtgI C'CryG_G
Cuts at: 0 185 390 1563
Size: 185 205 1173
Fragments arranged by size:
1173 205 185
BtsI GCAGTG_nn'
Cuts at: 0 705 1026 1563
Size: 705 321 537
Fragments arranged by size:
705 537 321
DraIII CAC_nnn'GTG
Cuts at: 0 1212 1563
Size: 1212 351
EaeI y'GGCC_r
Cuts at: 0 323 1563
Size: 323 1240
EarI CTCTTCn'nnn_
Cuts at: 0 811 1028 1563
Size: 811 217 535
Fragments arranged by size:
811 535 217
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 838 1563
Size: 838 725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1563
Size: 61 1502
EcoO109I rG'GnC_Cy
Cuts at: 0 190 1563
Size: 190 1373
EcoRV GAT'ATC
Cuts at: 0 522 1563
Size: 522 1041
HaeII r_GCGC'y
Cuts at: 0 218 1563
Size: 218 1345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 759 791 841 873 1147 1179 1563
Size: 759 32 50 32 274 32 384
Fragments arranged by size:
759 384 274 50 32 32 32
HincII GTy'rAC
Cuts at: 0 629 1563
Size: 629 934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 1563
Size: 938 625
MscI TGG'CCA
Cuts at: 0 325 1563
Size: 325 1238
MslI CAynn'nnrTG
Cuts at: 0 283 1060 1102 1563
Size: 283 777 42 461
Fragments arranged by size:
777 461 283 42
MspA1I CmG'CkG
Cuts at: 0 198 624 1563
Size: 198 426 939
Fragments arranged by size:
939 426 198
NspI r_CATG'y
Cuts at: 0 333 478 873 1563
Size: 333 145 395 690
Fragments arranged by size:
690 395 333 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 755 1314 1563
Size: 755 559 249
Fragments arranged by size:
755 559 249
PfoI T'CCnGG_A
Cuts at: 0 1250 1307 1563
Size: 1250 57 256
Fragments arranged by size:
1250 256 57
PpuMI rG'GwC_Cy
Cuts at: 0 190 1563
Size: 190 1373
PsiI TTA'TAA
Cuts at: 0 490 1563
Size: 490 1073
PstI C_TGCA'G
Cuts at: 0 935 1074 1269 1563
Size: 935 139 195 294
Fragments arranged by size:
935 294 195 139
SanDI GG'GwC_CC
Cuts at: 0 190 1563
Size: 190 1373
ScaI AGT'ACT
Cuts at: 0 971 1563
Size: 971 592
SfcI C'TryA_G
Cuts at: 0 68 414 507 597 931 1070 1265 1563
Size: 68 346 93 90 334 139 195 298
Fragments arranged by size:
346 334 298 195 139 93 90 68
SmlI C'TyrA_G
Cuts at: 0 712 1006 1563
Size: 712 294 557
Fragments arranged by size:
712 557 294
SphI G_CATG'C
Cuts at: 0 478 1563
Size: 478 1085
SspI AAT'ATT
Cuts at: 0 1498 1563
Size: 1498 65
TatI w'GTAC_w
Cuts at: 0 919 969 1416 1563
Size: 919 50 447 147
Fragments arranged by size:
919 447 147 50
XmnI GAAnn'nnTTC
Cuts at: 0 890 1563
Size: 890 673
Enzymes that do cut:
AccI AfeI AleI AlfI AloI AlwNI ApoI AseI
AvaI BamHI BcgI BplI BpmI BpuEI BsaI BsaBI
BsaXI BseRI BseYI BsrDI BsrGI BstYI BtgI BtsI
DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRV HaeII
Hin4I HincII MmeI MscI MslI MspA1I NspI PflMI
PfoI PpuMI PsiI PstI SanDI ScaI SfcI SmlI
SphI SspI TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI ApaI ApaLI AscI AsiSI AvrII BaeI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI Bpu10I BsaAI
BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI StyI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI