Restriction map for
pF1KE0003
(Linear) (Six-base) MAPSORT of: KIEE0003.seq Check: 6270 from: 1 to: 1542
KIEE0003.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 0 631 1542
Size: 631 911
AccI GT'mk_AC
Cuts at: 0 1430 1542
Size: 1430 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 511 1349 1542
Size: 321 190 838 193
Fragments arranged by size:
838 321 193 190
AflIII A'CryG_T
Cuts at: 0 1133 1542
Size: 1133 409
AlwNI CAG_nnn'CTG
Cuts at: 0 1501 1542
Size: 1501 41
ApoI r'AATT_y
Cuts at: 0 250 775 1542
Size: 250 525 767
Fragments arranged by size:
767 525 250
AvaI C'yCGr_G
Cuts at: 0 1005 1346 1542
Size: 1005 341 196
Fragments arranged by size:
1005 341 196
BanII G_rGCy'C
Cuts at: 0 1468 1542
Size: 1468 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 1379 1430 1542
Size: 1379 51 112
Fragments arranged by size:
1379 112 51
BclI T'GATC_A
Cuts at: 0 1503 1542
Size: 1503 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 558 692 780 1542
Size: 94 464 134 88 762
Fragments arranged by size:
762 464 134 94 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 1542
Size: 210 1332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1482 1542
Size: 1482 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 44 1542
Size: 44 1498
BsaBI GATnn'nnATC
Cuts at: 0 806 1542
Size: 806 736
BsaHI Gr'CG_yC
Cuts at: 0 628 1542
Size: 628 914
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 194 1542
Size: 194 1348
BseYI C'CCAG_C
Cuts at: 0 668 1542
Size: 668 874
BsiHKAI G_wGCw'C
Cuts at: 0 1468 1542
Size: 1468 74
BsmI GAATG_Cn'
Cuts at: 0 718 1542
Size: 718 824
Bsp1286I G_dGCh'C
Cuts at: 0 1468 1542
Size: 1468 74
BspHI T'CATG_A
Cuts at: 0 455 981 1542
Size: 455 526 561
Fragments arranged by size:
561 526 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1139 1542
Size: 1139 403
BstAPI GCAn_nnn'nTGC
Cuts at: 0 724 1542
Size: 724 818
BstYI r'GATC_y
Cuts at: 0 942 1542
Size: 942 600
DraI TTT'AAA
Cuts at: 0 1046 1542
Size: 1046 496
EarI CTCTTCn'nnn_
Cuts at: 0 361 1542
Size: 361 1181
EcoICRI GAG'CTC
Cuts at: 0 1466 1542
Size: 1466 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 321 511 1349 1542
Size: 210 111 190 838 193
Fragments arranged by size:
838 210 193 190 111
EcoO109I rG'GnC_Cy
Cuts at: 0 96 476 1542
Size: 96 380 1066
Fragments arranged by size:
1066 380 96
EcoRI G'AATT_C
Cuts at: 0 775 1542
Size: 775 767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 401 433 927 959 1074 1106
Size: 301 32 68 32 494 32 115 32
Cuts at: 1106 1542
Size: 436
Fragments arranged by size:
494 436 301 115 68 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1542
Size: 120 1422
MslI CAynn'nnrTG
Cuts at: 0 49 517 986 1297 1542
Size: 49 468 469 311 245
Fragments arranged by size:
469 468 311 245 49
MspA1I CmG'CkG
Cuts at: 0 668 1542
Size: 668 874
NdeI CA'TA_TG
Cuts at: 0 1119 1542
Size: 1119 423
NspI r_CATG'y
Cuts at: 0 837 1137 1542
Size: 837 300 405
Fragments arranged by size:
837 405 300
PciI A'CATG_T
Cuts at: 0 1133 1542
Size: 1133 409
PpuMI rG'GwC_Cy
Cuts at: 0 96 476 1542
Size: 96 380 1066
Fragments arranged by size:
1066 380 96
PsiI TTA'TAA
Cuts at: 0 974 1542
Size: 974 568
PstI C_TGCA'G
Cuts at: 0 471 1542
Size: 471 1071
PvuII CAG'CTG
Cuts at: 0 668 1542
Size: 668 874
SacI G_AGCT'C
Cuts at: 0 1468 1542
Size: 1468 74
SfcI C'TryA_G
Cuts at: 0 467 1542
Size: 467 1075
SmaI CCC'GGG
Cuts at: 0 1007 1542
Size: 1007 535
SmlI C'TyrA_G
Cuts at: 0 1346 1461 1542
Size: 1346 115 81
Fragments arranged by size:
1346 115 81
SpeI A'CTAG_T
Cuts at: 0 1486 1542
Size: 1486 56
SspI AAT'ATT
Cuts at: 0 86 968 1542
Size: 86 882 574
Fragments arranged by size:
882 574 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1372 1542
Size: 1372 170
TatI w'GTAC_w
Cuts at: 0 639 1528 1542
Size: 639 889 14
Fragments arranged by size:
889 639 14
Tth111I GACn'n_nGTC
Cuts at: 0 493 1542
Size: 493 1049
XhoI C'TCGA_G
Cuts at: 0 1346 1542
Size: 1346 196
XmaI C'CCGG_G
Cuts at: 0 1005 1542
Size: 1005 537
XmnI GAAnn'nnTTC
Cuts at: 0 865 1542
Size: 865 677
ZraI GAC'GTC
Cuts at: 0 629 1542
Size: 629 913
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApoI AvaI BanII
BbsI BclI BmrI BpmI BpuEI BsaI BsaBI BsaHI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BstAPI
BstYI DraI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
MmeI MslI MspA1I NdeI NspI PciI PpuMI PsiI
PstI PvuII SacI SfcI SmaI SmlI SpeI SspI
TaqII TatI Tth111I XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BsaAI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SphI SrfI StuI
StyI SwaI TstI XbaI XcmI