Restriction map for
pF1KB9986
(Linear) (Six-base) MAPSORT of: KIBB9986.seq Check: 2120 from: 1 to: 2136
KIBB9986.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 0 1104 2136
Size: 1104 1032
AccI GT'mk_AC
Cuts at: 0 1104 1296 2136
Size: 1104 192 840
Fragments arranged by size:
1104 840 192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 967 1124 1549 1573 1728 2136
Size: 358 609 157 425 24 155 408
Fragments arranged by size:
609 425 408 358 157 155 24
AflII C'TTAA_G
Cuts at: 0 1020 2136
Size: 1020 1116
AflIII A'CryG_T
Cuts at: 0 1321 2136
Size: 1321 815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1793 2136
Size: 1793 343
AlwNI CAG_nnn'CTG
Cuts at: 0 927 1377 1978 2136
Size: 927 450 601 158
Fragments arranged by size:
927 601 450 158
ApoI r'AATT_y
Cuts at: 0 223 258 296 727 1065 1642 1995 2136
Size: 223 35 38 431 338 577 353 141
Fragments arranged by size:
577 431 353 338 223 141 38 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 586 1246 1576 2104 2136
Size: 586 660 330 528 32
Fragments arranged by size:
660 586 528 330 32
BbvCI CC'TCA_GC
Cuts at: 0 1378 2136
Size: 1378 758
BciVI GTATCCnnnnn_n'
Cuts at: 0 1295 2136
Size: 1295 841
BfrBI ATG'CAT
Cuts at: 0 1360 2136
Size: 1360 776
BglI GCCn_nnn'nGGC
Cuts at: 0 1560 2136
Size: 1560 576
BglII A'GATC_T
Cuts at: 0 62 2136
Size: 62 2074
BlpI GC'TnA_GC
Cuts at: 0 598 2136
Size: 598 1538
BmgBI CAC'GTC
Cuts at: 0 513 2136
Size: 513 1623
BmrI ACTGGGnnnn_n'
Cuts at: 0 1491 2136
Size: 1491 645
Bpu10I CC'TnA_GC
Cuts at: 0 1378 2136
Size: 1378 758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 1816 2136
Size: 1435 381 320
Fragments arranged by size:
1435 381 320
BsaAI yAC'GTr
Cuts at: 0 1125 2136
Size: 1125 1011
BsaHI Gr'CG_yC
Cuts at: 0 1101 2136
Size: 1101 1035
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1709 1739 2136
Size: 1709 30 397
Fragments arranged by size:
1709 397 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 626 1366 1498 2136
Size: 511 115 740 132 638
Fragments arranged by size:
740 638 511 132 115
BseYI C'CCAG_C
Cuts at: 0 1013 2136
Size: 1013 1123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1790 1891 2136
Size: 1790 101 245
Fragments arranged by size:
1790 245 101
BsiHKAI G_wGCw'C
Cuts at: 0 851 2136
Size: 851 1285
BsmI GAATG_Cn'
Cuts at: 0 1865 2136
Size: 1865 271
Bsp1286I G_dGCh'C
Cuts at: 0 851 2136
Size: 851 1285
BspHI T'CATG_A
Cuts at: 0 339 948 2136
Size: 339 609 1188
Fragments arranged by size:
1188 609 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1220 2136
Size: 1220 916
BsrDI GCAATG_nn'
Cuts at: 0 403 2136
Size: 403 1733
BsrGI T'GTAC_A
Cuts at: 0 1318 2136
Size: 1318 818
BssSI C'ACGA_G
Cuts at: 0 1936 2136
Size: 1936 200
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1999 2136
Size: 1999 137
BstBI TT'CG_AA
Cuts at: 0 131 2136
Size: 131 2005
BstYI r'GATC_y
Cuts at: 0 62 2136
Size: 62 2074
Bsu36I CC'TnA_GG
Cuts at: 0 110 2136
Size: 110 2026
DraI TTT'AAA
Cuts at: 0 900 1775 2136
Size: 900 875 361
Fragments arranged by size:
900 875 361
EarI CTCTTCn'nnn_
Cuts at: 0 1275 1280 2136
Size: 1275 5 856
Fragments arranged by size:
1275 856 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 967 1124 1549 1573 1728 2136
Size: 358 609 157 425 24 155 408
Fragments arranged by size:
609 425 408 358 157 155 24
EcoO109I rG'GnC_Cy
Cuts at: 0 369 2136
Size: 369 1767
EcoRI G'AATT_C
Cuts at: 0 258 2136
Size: 258 1878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 45 632 2136
Size: 45 587 1504
Fragments arranged by size:
1504 587 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 1345 1377 1707 1739 2136
Size: 472 32 841 32 330 32 397
Fragments arranged by size:
841 472 397 330 32 32 32
HincII GTy'rAC
Cuts at: 0 715 1297 2136
Size: 715 582 839
Fragments arranged by size:
839 715 582
HindIII A'AGCT_T
Cuts at: 0 121 669 1136 2136
Size: 121 548 467 1000
Fragments arranged by size:
1000 548 467 121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1122 2136
Size: 325 797 1014
Fragments arranged by size:
1014 797 325
MslI CAynn'nnrTG
Cuts at: 0 722 1775 2136
Size: 722 1053 361
Fragments arranged by size:
1053 722 361
MspA1I CmG'CkG
Cuts at: 0 1374 1975 2136
Size: 1374 601 161
Fragments arranged by size:
1374 601 161
NdeI CA'TA_TG
Cuts at: 0 393 1226 1339 2136
Size: 393 833 113 797
Fragments arranged by size:
833 797 393 113
NsiI A_TGCA'T
Cuts at: 0 1362 2136
Size: 1362 774
NspI r_CATG'y
Cuts at: 0 1219 1325 2136
Size: 1219 106 811
Fragments arranged by size:
1219 811 106
PciI A'CATG_T
Cuts at: 0 1321 2136
Size: 1321 815
PflMI CCAn_nnn'nTGG
Cuts at: 0 1734 2136
Size: 1734 402
PsiI TTA'TAA
Cuts at: 0 973 2080 2136
Size: 973 1107 56
Fragments arranged by size:
1107 973 56
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 908 940 2136
Size: 908 32 1196
Fragments arranged by size:
1196 908 32
PvuII CAG'CTG
Cuts at: 0 1374 1975 2136
Size: 1374 601 161
Fragments arranged by size:
1374 601 161
SalI G'TCGA_C
Cuts at: 0 1295 2136
Size: 1295 841
ScaI AGT'ACT
Cuts at: 0 686 1652 2136
Size: 686 966 484
Fragments arranged by size:
966 686 484
SmlI C'TyrA_G
Cuts at: 0 1020 1414 1831 2136
Size: 1020 394 417 305
Fragments arranged by size:
1020 417 394 305
StyI C'CwwG_G
Cuts at: 0 1458 2136
Size: 1458 678
SwaI ATTT'AAAT
Cuts at: 0 1775 2136
Size: 1775 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 2136
Size: 532 1604
TatI w'GTAC_w
Cuts at: 0 684 1313 1318 1650 1739 2136
Size: 684 629 5 332 89 397
Fragments arranged by size:
684 629 397 332 89 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1709 1741 2136
Size: 1709 32 395
Fragments arranged by size:
1709 395 32
XmnI GAAnn'nnTTC
Cuts at: 0 506 1234 1423 1700 2136
Size: 506 728 189 277 436
Fragments arranged by size:
728 506 436 277 189
ZraI GAC'GTC
Cuts at: 0 1102 2136
Size: 1102 1034
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AlfI AlwNI ApoI
BbsI BbvCI BciVI BfrBI BglI BglII BlpI BmgBI
BmrI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BssSI BstAPI BstBI BstYI Bsu36I DraI EarI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MslI
MspA1I NdeI NsiI NspI PciI PflMI PsiI PsrI
PvuII SalI ScaI SmlI StyI SwaI TaqII TatI
TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BcgI BclI Bme1580I BmtI
BplI BpmI BsaI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BstEII BstXI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI RsrII SacI SacII SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI Tth111I XbaI XcmI XhoI
XmaI