(Linear) (Six-base) MAPSORT of: KIBB9983.seq Check: 8939 from: 1 to: 1710 KIBB9983.orf With 182 enzymes: SgfI * April 24, 2009 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 364 1710 Size: 364 1346 Acc65I G'GTAC_C Cuts at: 0 844 1710 Size: 844 866 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 614 1710 Size: 614 1096 AflIII A'CryG_T Cuts at: 0 1471 1710 Size: 1471 239 AgeI A'CCGG_T Cuts at: 0 784 1710 Size: 784 926 AlwNI CAG_nnn'CTG Cuts at: 0 238 356 1710 Size: 238 118 1354 Fragments arranged by size: 1354 238 118 ApaLI G'TGCA_C Cuts at: 0 1510 1710 Size: 1510 200 ApoI r'AATT_y Cuts at: 0 624 1500 1710 Size: 624 876 210 Fragments arranged by size: 876 624 210 AvaI C'yCGr_G Cuts at: 0 464 1710 Size: 464 1246 BanI G'GyrC_C Cuts at: 0 844 1367 1477 1710 Size: 844 523 110 233 Fragments arranged by size: 844 523 233 110 BbeI G_GCGC'C Cuts at: 0 1481 1710 Size: 1481 229 BbsI GAAGACnn'nnnn_ Cuts at: 0 998 1710 Size: 998 712 BciVI GTATCCnnnnn_n' Cuts at: 0 1130 1710 Size: 1130 580 BclI T'GATC_A Cuts at: 0 945 1710 Size: 945 765 BfrBI ATG'CAT Cuts at: 0 1332 1710 Size: 1332 378 Bme1580I G_kGCm'C Cuts at: 0 132 1514 1710 Size: 132 1382 196 Fragments arranged by size: 1382 196 132 BmgBI CAC'GTC Cuts at: 0 1067 1710 Size: 1067 643 BmtI G_CTAG'C Cuts at: 0 1549 1710 Size: 1549 161 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 497 1710 Size: 497 1213 Bpu10I CC'TnA_GC Cuts at: 0 675 1710 Size: 675 1035 BsaI GGTCTCn'nnnn_ Cuts at: 0 775 1603 1710 Size: 775 828 107 Fragments arranged by size: 828 775 107 BsaBI GATnn'nnATC Cuts at: 0 221 1097 1710 Size: 221 876 613 Fragments arranged by size: 876 613 221 BsaHI Gr'CG_yC Cuts at: 0 1478 1710 Size: 1478 232 BsaWI w'CCGG_w Cuts at: 0 784 1086 1364 1581 1710 Size: 784 302 278 217 129 Fragments arranged by size: 784 302 278 217 129 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1065 1095 1710 Size: 1065 30 615 Fragments arranged by size: 1065 615 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 18 904 1073 1533 1647 1710 Size: 18 886 169 460 114 63 Fragments arranged by size: 886 460 169 114 63 18 BseYI C'CCAG_C Cuts at: 0 911 1710 Size: 911 799 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 519 1710 Size: 519 1191 BsiHKAI G_wGCw'C Cuts at: 0 823 1514 1710 Size: 823 691 196 Fragments arranged by size: 823 691 196 Bsp1286I G_dGCh'C Cuts at: 0 132 823 1514 1710 Size: 132 691 691 196 Fragments arranged by size: 691 691 196 132 BspEI T'CCGG_A Cuts at: 0 1086 1710 Size: 1086 624 BspHI T'CATG_A Cuts at: 0 368 1710 Size: 368 1342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 364 1216 1710 Size: 364 852 494 Fragments arranged by size: 852 494 364 BsrDI GCAATG_nn' Cuts at: 0 1136 1710 Size: 1136 574 BsrFI r'CCGG_y Cuts at: 0 784 1710 Size: 784 926 BstAPI GCAn_nnn'nTGC Cuts at: 0 1251 1710 Size: 1251 459 BstYI r'GATC_y Cuts at: 0 216 1710 Size: 216 1494 Bsu36I CC'TnA_GG Cuts at: 0 1516 1710 Size: 1516 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1318 1710 Size: 1318 392 BtsI GCAGTG_nn' Cuts at: 0 162 507 1572 1710 Size: 162 345 1065 138 Fragments arranged by size: 1065 345 162 138 ClaI AT'CG_AT Cuts at: 0 1101 1710 Size: 1101 609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1455 1490 1710 Size: 1455 35 220 Fragments arranged by size: 1455 220 35 EarI CTCTTCn'nnn_ Cuts at: 0 36 675 1601 1710 Size: 36 639 926 109 Fragments arranged by size: 926 639 109 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 614 1710 Size: 614 1096 EcoO109I rG'GnC_Cy Cuts at: 0 984 1710 Size: 984 726 EcoRI G'AATT_C Cuts at: 0 624 1710 Size: 624 1086 EcoRV GAT'ATC Cuts at: 0 96 1710 Size: 96 1614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1003 1630 1710 Size: 1003 627 80 Fragments arranged by size: 1003 627 80 HaeII r_GCGC'y Cuts at: 0 1481 1710 Size: 1481 229 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 892 924 1710 Size: 892 32 786 Fragments arranged by size: 892 786 32 HincII GTy'rAC Cuts at: 0 666 1710 Size: 666 1044 KasI G'GCGC_C Cuts at: 0 1477 1710 Size: 1477 233 KpnI G_GTAC'C Cuts at: 0 848 1710 Size: 848 862 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 194 1228 1710 Size: 194 1034 482 Fragments arranged by size: 1034 482 194 MslI CAynn'nnrTG Cuts at: 0 89 565 1281 1710 Size: 89 476 716 429 Fragments arranged by size: 716 476 429 89 MspA1I CmG'CkG Cuts at: 0 1292 1710 Size: 1292 418 NarI GG'CG_CC Cuts at: 0 1478 1710 Size: 1478 232 NheI G'CTAG_C Cuts at: 0 1545 1710 Size: 1545 165 NsiI A_TGCA'T Cuts at: 0 1334 1710 Size: 1334 376 NspI r_CATG'y Cuts at: 0 1254 1475 1710 Size: 1254 221 235 Fragments arranged by size: 1254 235 221 PciI A'CATG_T Cuts at: 0 1471 1710 Size: 1471 239 PfoI T'CCnGG_A Cuts at: 0 110 538 960 1710 Size: 110 428 422 750 Fragments arranged by size: 750 428 422 110 PpuMI rG'GwC_Cy Cuts at: 0 984 1710 Size: 984 726 SfcI C'TryA_G Cuts at: 0 1013 1710 Size: 1013 697 SfoI GGC'GCC Cuts at: 0 1479 1710 Size: 1479 231 SmlI C'TyrA_G Cuts at: 0 464 1710 Size: 464 1246 SspI AAT'ATT Cuts at: 0 300 1710 Size: 300 1410 StyI C'CwwG_G Cuts at: 0 685 1710 Size: 685 1025 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 411 1710 Size: 411 1299 TatI w'GTAC_w Cuts at: 0 1156 1710 Size: 1156 554 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1065 1097 1710 Size: 1065 32 613 Fragments arranged by size: 1065 613 32 Tth111I GACn'n_nGTC Cuts at: 0 1169 1710 Size: 1169 541 XbaI T'CTAG_A Cuts at: 0 420 1710 Size: 420 1290 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 49 1710 Size: 49 1661 XhoI C'TCGA_G Cuts at: 0 464 1710 Size: 464 1246 XmnI GAAnn'nnTTC Cuts at: 0 1112 1303 1710 Size: 1112 191 407 Fragments arranged by size: 1112 407 191 Enzymes that do cut: AarI Acc65I AcuI AflIII AgeI AlwNI ApaLI ApoI AvaI BanI BbeI BbsI BciVI BclI BfrBI Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstAPI BstYI Bsu36I BtgZI BtsI ClaI CspCI EarI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII KasI KpnI MmeI MslI MspA1I NarI NheI NsiI NspI PciI PfoI PpuMI SfcI SfoI SmlI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BglI BglII BlpI BmrI BpmI BpuEI BsaAI BsiEI BsiWI BsmI BsmBI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI FseI FspI FspAI HindIII HpaI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NotI NruI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI XmaI ZraI