Restriction map for
pF1KB9979
(Linear) (Six-base) MAPSORT of: KIBB9979.seq Check: 1917 from: 1 to: 1674
KIBB9979.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1123 1674
Size: 706 417 551
Fragments arranged by size:
706 551 417
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1012 1674
Size: 1012 662
AlwNI CAG_nnn'CTG
Cuts at: 0 1101 1398 1674
Size: 1101 297 276
Fragments arranged by size:
1101 297 276
ApaI G_GGCC'C
Cuts at: 0 924 1674
Size: 924 750
ApaLI G'TGCA_C
Cuts at: 0 899 1674
Size: 899 775
ApoI r'AATT_y
Cuts at: 0 263 309 1674
Size: 263 46 1365
Fragments arranged by size:
1365 263 46
AvaI C'yCGr_G
Cuts at: 0 529 1079 1640 1674
Size: 529 550 561 34
Fragments arranged by size:
561 550 529 34
BanI G'GyrC_C
Cuts at: 0 734 1463 1674
Size: 734 729 211
Fragments arranged by size:
734 729 211
BanII G_rGCy'C
Cuts at: 0 924 1443 1494 1649 1674
Size: 924 519 51 155 25
Fragments arranged by size:
924 519 155 51 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 97 1145 1674
Size: 97 1048 529
Fragments arranged by size:
1048 529 97
BciVI GTATCCnnnnn_n'
Cuts at: 0 332 1674
Size: 332 1342
BclI T'GATC_A
Cuts at: 0 1457 1674
Size: 1457 217
BglII A'GATC_T
Cuts at: 0 18 1674
Size: 18 1656
Bme1580I G_kGCm'C
Cuts at: 0 903 924 1423 1674
Size: 903 21 499 251
Fragments arranged by size:
903 499 251 21
BmgBI CAC'GTC
Cuts at: 0 625 1674
Size: 625 1049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 771 997 1078 1517 1674
Size: 628 143 226 81 439 157
Fragments arranged by size:
628 439 226 157 143 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 830 994 1052 1652 1674
Size: 389 441 164 58 600 22
Fragments arranged by size:
600 441 389 164 58 22
BsaAI yAC'GTr
Cuts at: 0 111 1674
Size: 111 1563
BsaBI GATnn'nnATC
Cuts at: 0 434 1674
Size: 434 1240
BsaHI Gr'CG_yC
Cuts at: 0 541 1674
Size: 541 1133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1076 1106 1674
Size: 1076 30 568
Fragments arranged by size:
1076 568 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 1462 1674
Size: 598 864 212
Fragments arranged by size:
864 598 212
BseYI C'CCAG_C
Cuts at: 0 89 742 1347 1674
Size: 89 653 605 327
Fragments arranged by size:
653 605 327 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1674
Size: 1430 244
BsiEI CG_ry'CG
Cuts at: 0 1511 1674
Size: 1511 163
BsiHKAI G_wGCw'C
Cuts at: 0 903 1320 1443 1649 1674
Size: 903 417 123 206 25
Fragments arranged by size:
903 417 206 123 25
BsmI GAATG_Cn'
Cuts at: 0 823 1674
Size: 823 851
Bsp1286I G_dGCh'C
Cuts at: 0 903 924 1320 1423 1443 1494 1649 1674
Size: 903 21 396 103 20 51 155 25
Fragments arranged by size:
903 396 155 103 51 25 21 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1406 1674
Size: 1406 268
BsrDI GCAATG_nn'
Cuts at: 0 764 1370 1674
Size: 764 606 304
Fragments arranged by size:
764 606 304
BsrGI T'GTAC_A
Cuts at: 0 1589 1674
Size: 1589 85
BssSI C'ACGA_G
Cuts at: 0 551 1558 1674
Size: 551 1007 116
Fragments arranged by size:
1007 551 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1150 1674
Size: 1150 524
BstYI r'GATC_y
Cuts at: 0 18 1674
Size: 18 1656
Bsu36I CC'TnA_GG
Cuts at: 0 133 632 1674
Size: 133 499 1042
Fragments arranged by size:
1042 499 133
BtgI C'CryG_G
Cuts at: 0 1046 1256 1674
Size: 1046 210 418
Fragments arranged by size:
1046 418 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 821 1674
Size: 821 853
BtsI GCAGTG_nn'
Cuts at: 0 964 1541 1674
Size: 964 577 133
Fragments arranged by size:
964 577 133
DrdI GACnn_nn'nnGTC
Cuts at: 0 1152 1674
Size: 1152 522
EcoICRI GAG'CTC
Cuts at: 0 1441 1647 1674
Size: 1441 206 27
Fragments arranged by size:
1441 206 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 706 771 997 1078 1123 1517 1674
Size: 628 78 65 226 81 45 394 157
Fragments arranged by size:
628 394 226 157 81 78 65 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 598 1674
Size: 598 1076
EcoO109I rG'GnC_Cy
Cuts at: 0 920 921 1154 1165 1395 1674
Size: 920 1 233 11 230 279
Fragments arranged by size:
920 279 233 230 11 1
EcoRI G'AATT_C
Cuts at: 0 309 1674
Size: 309 1365
EcoRV GAT'ATC
Cuts at: 0 1074 1674
Size: 1074 600
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 412 1035 1674
Size: 412 623 639
Fragments arranged by size:
639 623 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 786 818 1076 1108 1674
Size: 545 32 209 32 258 32 566
Fragments arranged by size:
566 545 258 209 32 32 32
HincII GTy'rAC
Cuts at: 0 205 588 1674
Size: 205 383 1086
Fragments arranged by size:
1086 383 205
HindIII A'AGCT_T
Cuts at: 0 52 218 1674
Size: 52 166 1456
Fragments arranged by size:
1456 166 52
MfeI C'AATT_G
Cuts at: 0 197 1674
Size: 197 1477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 1674
Size: 205 1469
MslI CAynn'nnrTG
Cuts at: 0 497 623 1531 1674
Size: 497 126 908 143
Fragments arranged by size:
908 497 143 126
MspA1I CmG'CkG
Cuts at: 0 712 996 1455 1674
Size: 712 284 459 219
Fragments arranged by size:
712 459 284 219
NcoI C'CATG_G
Cuts at: 0 1046 1674
Size: 1046 628
NdeI CA'TA_TG
Cuts at: 0 246 1674
Size: 246 1428
PflMI CCAn_nnn'nTGG
Cuts at: 0 1359 1674
Size: 1359 315
PfoI T'CCnGG_A
Cuts at: 0 122 334 905 1494 1674
Size: 122 212 571 589 180
Fragments arranged by size:
589 571 212 180 122
PmlI CAC'GTG
Cuts at: 0 111 1674
Size: 111 1563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1674
Size: 1165 32 477
Fragments arranged by size:
1165 477 32
PpuMI rG'GwC_Cy
Cuts at: 0 1154 1165 1395 1674
Size: 1154 11 230 279
Fragments arranged by size:
1154 279 230 11
PshAI GACnn'nnGTC
Cuts at: 0 101 1327 1674
Size: 101 1226 347
Fragments arranged by size:
1226 347 101
PsiI TTA'TAA
Cuts at: 0 826 1674
Size: 826 848
PspOMI G'GGCC_C
Cuts at: 0 920 1674
Size: 920 754
PstI C_TGCA'G
Cuts at: 0 1403 1674
Size: 1403 271
PvuI CG_AT'CG
Cuts at: 0 1511 1674
Size: 1511 163
PvuII CAG'CTG
Cuts at: 0 712 996 1455 1674
Size: 712 284 459 219
Fragments arranged by size:
712 459 284 219
SacI G_AGCT'C
Cuts at: 0 1443 1649 1674
Size: 1443 206 25
Fragments arranged by size:
1443 206 25
SfcI C'TryA_G
Cuts at: 0 425 713 1399 1674
Size: 425 288 686 275
Fragments arranged by size:
686 425 288 275
SmlI C'TyrA_G
Cuts at: 0 5 404 845 1009 1067 1667 1674
Size: 5 399 441 164 58 600 7
Fragments arranged by size:
600 441 399 164 58 7 5
StyI C'CwwG_G
Cuts at: 0 1046 1674
Size: 1046 628
TatI w'GTAC_w
Cuts at: 0 1589 1674
Size: 1589 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 330 362 1674
Size: 330 32 1312
Fragments arranged by size:
1312 330 32
Enzymes that do cut:
AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbsI BciVI BclI BglII Bme1580I BmgBI BpmI
BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BssSI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII MfeI MmeI MslI MspA1I NcoI NdeI PflMI
PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SfcI SmlI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BlpI
BmrI BmtI BplI Bpu10I BsaI BsaWI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BstAPI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII EaeI EagI EarI
EciI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PspXI PsrI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI