Restriction map for
pF1KB9975
(Linear) (Six-base) MAPSORT of: KIBB9975.seq Check: 7518 from: 1 to: 1593
KIBB9975.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1571 1593
Size: 1571 22
AatII G_ACGT'C
Cuts at: 0 209 1593
Size: 209 1384
AccI GT'mk_AC
Cuts at: 0 1079 1593
Size: 1079 514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 427 1593
Size: 292 135 1166
Fragments arranged by size:
1166 292 135
AleI CACnn'nnGTG
Cuts at: 0 328 346 1593
Size: 328 18 1247
Fragments arranged by size:
1247 328 18
AlwNI CAG_nnn'CTG
Cuts at: 0 672 1275 1593
Size: 672 603 318
Fragments arranged by size:
672 603 318
ApaI G_GGCC'C
Cuts at: 0 441 1593
Size: 441 1152
ApoI r'AATT_y
Cuts at: 0 1005 1593
Size: 1005 588
AvaI C'yCGr_G
Cuts at: 0 984 1546 1593
Size: 984 562 47
Fragments arranged by size:
984 562 47
BanI G'GyrC_C
Cuts at: 0 916 1593
Size: 916 677
BanII G_rGCy'C
Cuts at: 0 441 544 1327 1498 1593
Size: 441 103 783 171 95
Fragments arranged by size:
783 441 171 103 95
BbeI G_GCGC'C
Cuts at: 0 920 1593
Size: 920 673
BbsI GAAGACnn'nnnn_
Cuts at: 0 415 1593
Size: 415 1178
BglI GCCn_nnn'nGGC
Cuts at: 0 445 454 502 931 1165 1593
Size: 445 9 48 429 234 428
Fragments arranged by size:
445 429 428 234 48 9
Bme1580I G_kGCm'C
Cuts at: 0 441 1190 1593
Size: 441 749 403
Fragments arranged by size:
749 441 403
BmgBI CAC'GTC
Cuts at: 0 1089 1593
Size: 1089 504
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 75 1593
Size: 75 1518
Bpu10I CC'TnA_GC
Cuts at: 0 550 1593
Size: 550 1043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1593
Size: 1393 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 1575 1593
Size: 1575 18
BsaBI GATnn'nnATC
Cuts at: 0 184 979 1593
Size: 184 795 614
Fragments arranged by size:
795 614 184
BsaHI Gr'CG_yC
Cuts at: 0 164 206 917 1076 1436 1593
Size: 164 42 711 159 360 157
Fragments arranged by size:
711 360 164 159 157 42
BsaWI w'CCGG_w
Cuts at: 0 188 1593
Size: 188 1405
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 1414 1519 1593
Size: 325 1089 105 74
Fragments arranged by size:
1089 325 105 74
BseYI C'CCAG_C
Cuts at: 0 701 1160 1593
Size: 701 459 433
Fragments arranged by size:
701 459 433
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 583 735 1111 1156 1180 1261 1288
Size: 179 404 152 376 45 24 81 27
Cuts at: 1288 1351 1516 1593
Size: 63 165 77
Fragments arranged by size:
404 376 179 165 152 81 77 63
45 27 24
BsiEI CG_ry'CG
Cuts at: 0 509 1593
Size: 509 1084
BsiHKAI G_wGCw'C
Cuts at: 0 551 1593
Size: 551 1042
Bsp1286I G_dGCh'C
Cuts at: 0 441 544 551 1190 1327 1498 1593
Size: 441 103 7 639 137 171 95
Fragments arranged by size:
639 441 171 137 103 95 7
BspEI T'CCGG_A
Cuts at: 0 188 1593
Size: 188 1405
BspHI T'CATG_A
Cuts at: 0 89 1593
Size: 89 1504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 845 958 1538 1571 1593
Size: 845 113 580 33 22
Fragments arranged by size:
845 580 113 33 22
BsrDI GCAATG_nn'
Cuts at: 0 1434 1593
Size: 1434 159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1194 1593
Size: 1194 399
BstEII G'GTnAC_C
Cuts at: 0 252 348 1593
Size: 252 96 1245
Fragments arranged by size:
1245 252 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 327 1593
Size: 327 1266
BstYI r'GATC_y
Cuts at: 0 43 1103 1404 1593
Size: 43 1060 301 189
Fragments arranged by size:
1060 301 189 43
BtgI C'CryG_G
Cuts at: 0 326 509 875 881 912 1499 1593
Size: 326 183 366 6 31 587 94
Fragments arranged by size:
587 366 326 183 94 31 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 541 1593
Size: 541 1052
DrdI GACnn_nn'nnGTC
Cuts at: 0 10 1593
Size: 10 1583
EaeI y'GGCC_r
Cuts at: 0 366 506 530 878 932 1166 1358 1593
Size: 366 140 24 348 54 234 192 235
Fragments arranged by size:
366 348 235 234 192 140 54 24
EagI C'GGCC_G
Cuts at: 0 506 1593
Size: 506 1087
EarI CTCTTCn'nnn_
Cuts at: 0 577 1593
Size: 577 1016
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1159 1593
Size: 1159 434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 427 1593
Size: 292 135 1166
Fragments arranged by size:
1166 292 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1279 1593
Size: 1279 314
EcoO109I rG'GnC_Cy
Cuts at: 0 669 1272 1413 1518 1593
Size: 669 603 141 105 75
Fragments arranged by size:
669 603 141 105 75
FspI TGC'GCA
Cuts at: 0 760 1593
Size: 760 833
HaeII r_GCGC'y
Cuts at: 0 701 920 1593
Size: 701 219 673
Fragments arranged by size:
701 673 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 1593
Size: 71 32 1490
Fragments arranged by size:
1490 71 32
KasI G'GCGC_C
Cuts at: 0 916 1593
Size: 916 677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 952 1400 1593
Size: 416 536 448 193
Fragments arranged by size:
536 448 416 193
MscI TGG'CCA
Cuts at: 0 934 1168 1593
Size: 934 234 425
Fragments arranged by size:
934 425 234
MslI CAynn'nnrTG
Cuts at: 0 298 328 346 1593
Size: 298 30 18 1247
Fragments arranged by size:
1247 298 30 18
MspA1I CmG'CkG
Cuts at: 0 883 958 1164 1245 1593
Size: 883 75 206 81 348
Fragments arranged by size:
883 348 206 81 75
NarI GG'CG_CC
Cuts at: 0 917 1593
Size: 917 676
NcoI C'CATG_G
Cuts at: 0 326 875 1593
Size: 326 549 718
Fragments arranged by size:
718 549 326
PasI CC'CwG_GG
Cuts at: 0 231 1593
Size: 231 1362
PflMI CCAn_nnn'nTGG
Cuts at: 0 876 1593
Size: 876 717
PpuMI rG'GwC_Cy
Cuts at: 0 1413 1593
Size: 1413 180
PspOMI G'GGCC_C
Cuts at: 0 437 1593
Size: 437 1156
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 559 591 1593
Size: 559 32 1002
Fragments arranged by size:
1002 559 32
PstI C_TGCA'G
Cuts at: 0 114 455 680 1593
Size: 114 341 225 913
Fragments arranged by size:
913 341 225 114
PvuII CAG'CTG
Cuts at: 0 958 1164 1245 1593
Size: 958 206 81 348
Fragments arranged by size:
958 348 206 81
RsrII CG'GwC_CG
Cuts at: 0 79 1593
Size: 79 1514
SacII CC_GC'GG
Cuts at: 0 884 1593
Size: 884 709
SbfI CC_TGCA'GG
Cuts at: 0 455 1593
Size: 455 1138
SfcI C'TryA_G
Cuts at: 0 110 451 676 1593
Size: 110 341 225 917
Fragments arranged by size:
917 341 225 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 445 931 1165 1593
Size: 445 486 234 428
Fragments arranged by size:
486 445 428 234
SfoI GGC'GCC
Cuts at: 0 918 1593
Size: 918 675
SmaI CCC'GGG
Cuts at: 0 986 1593
Size: 986 607
SmlI C'TyrA_G
Cuts at: 0 1408 1593
Size: 1408 185
StuI AGG'CCT
Cuts at: 0 289 1593
Size: 289 1304
StyI C'CwwG_G
Cuts at: 0 326 488 875 1280 1593
Size: 326 162 387 405 313
Fragments arranged by size:
405 387 326 313 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 456 1593
Size: 427 29 1137
Fragments arranged by size:
1137 427 29
TatI w'GTAC_w
Cuts at: 0 797 1593
Size: 797 796
XbaI T'CTAG_A
Cuts at: 0 172 1593
Size: 172 1421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 987 1593
Size: 717 270 606
Fragments arranged by size:
717 606 270
XmaI C'CCGG_G
Cuts at: 0 984 1593
Size: 984 609
XmnI GAAnn'nnTTC
Cuts at: 0 571 1345 1593
Size: 571 774 248
Fragments arranged by size:
774 571 248
ZraI GAC'GTC
Cuts at: 0 207 1593
Size: 207 1386
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BglI Bme1580I BmgBI
BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI
NcoI PasI PflMI PpuMI PspOMI PsrI PstI PvuII
RsrII SacII SbfI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BpmI BsaAI BsaXI BsiWI BsmI BsmBI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII EcoICRI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI SacI SalI SanDI SapI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XhoI