Restriction map for
pF1KB9974
(Linear) (Six-base) MAPSORT of: KIBB9974.seq Check: 7515 from: 1 to: 2121
KIBB9974.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 0 1991 2121
Size: 1991 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 2121
Size: 292 1829
AgeI A'CCGG_T
Cuts at: 0 247 797 2121
Size: 247 550 1324
Fragments arranged by size:
1324 550 247
AleI CACnn'nnGTG
Cuts at: 0 82 166 472 1516 2121
Size: 82 84 306 1044 605
Fragments arranged by size:
1044 605 306 84 82
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1942 1974 2121
Size: 1942 32 147
Fragments arranged by size:
1942 147 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 2121
Size: 18 2103
ApaI G_GGCC'C
Cuts at: 0 1286 1657 1792 1848 2121
Size: 1286 371 135 56 273
Fragments arranged by size:
1286 371 273 135 56
AvaI C'yCGr_G
Cuts at: 0 164 241 833 992 1791 1963 2121
Size: 164 77 592 159 799 172 158
Fragments arranged by size:
799 592 172 164 159 158 77
BamHI G'GATC_C
Cuts at: 0 371 1926 2121
Size: 371 1555 195
Fragments arranged by size:
1555 371 195
BanI G'GyrC_C
Cuts at: 0 347 1106 1319 1751 1889 1968 2121
Size: 347 759 213 432 138 79 153
Fragments arranged by size:
759 432 347 213 153 138 79
BanII G_rGCy'C
Cuts at: 0 560 1286 1657 1792 1848 2121
Size: 560 726 371 135 56 273
Fragments arranged by size:
726 560 371 273 135 56
BbeI G_GCGC'C
Cuts at: 0 1110 1755 1893 2121
Size: 1110 645 138 228
Fragments arranged by size:
1110 645 228 138
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 1035 1983 2121
Size: 395 640 948 138
Fragments arranged by size:
948 640 395 138
BbvCI CC'TCA_GC
Cuts at: 0 1467 2121
Size: 1467 654
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 437 627 661 2121
Size: 403 34 190 34 1460
Fragments arranged by size:
1460 403 190 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 545 1168 2121
Size: 545 623 953
Fragments arranged by size:
953 623 545
BglI GCCn_nnn'nGGC
Cuts at: 0 1828 2121
Size: 1828 293
Bme1580I G_kGCm'C
Cuts at: 0 1286 1657 1792 1848 2113 2121
Size: 1286 371 135 56 265 8
Fragments arranged by size:
1286 371 265 135 56 8
BmgBI CAC'GTC
Cuts at: 0 1305 1827 1908 2121
Size: 1305 522 81 213
Fragments arranged by size:
1305 522 213 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 732 1740 2121
Size: 732 1008 381
Fragments arranged by size:
1008 732 381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 205 1917 2121
Size: 116 89 1712 204
Fragments arranged by size:
1712 204 116 89
Bpu10I CC'TnA_GC
Cuts at: 0 1346 1467 2121
Size: 1346 121 654
Fragments arranged by size:
1346 654 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 2121
Size: 325 1796
BsaI GGTCTCn'nnnn_
Cuts at: 0 92 238 2121
Size: 92 146 1883
Fragments arranged by size:
1883 146 92
BsaAI yAC'GTr
Cuts at: 0 2088 2121
Size: 2088 33
BsaHI Gr'CG_yC
Cuts at: 0 416 454 602 644 1107 1565 1752 1797
Size: 416 38 148 42 463 458 187 45
Cuts at: 1797 1890 1988 2121
Size: 93 98 133
Fragments arranged by size:
463 458 416 187 148 133 98 93
45 42 38
BsaWI w'CCGG_w
Cuts at: 0 247 797 1408 1530 1850 1952 2121
Size: 247 550 611 122 320 102 169
Fragments arranged by size:
611 550 320 247 169 122 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 904 934 1811 1841 2121
Size: 730 30 144 30 877 30 280
Fragments arranged by size:
877 730 280 144 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1729 2121
Size: 1729 392
BseYI C'CCAG_C
Cuts at: 0 39 814 1218 1785 1809 2121
Size: 39 775 404 567 24 312
Fragments arranged by size:
775 567 404 312 39 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 786 888 2121
Size: 714 72 102 1233
Fragments arranged by size:
1233 714 102 72
BsiEI CG_ry'CG
Cuts at: 0 744 798 918 963 1648 2121
Size: 744 54 120 45 685 473
Fragments arranged by size:
744 685 473 120 54 45
BsiHKAI G_wGCw'C
Cuts at: 0 32 89 524 2121
Size: 32 57 435 1597
Fragments arranged by size:
1597 435 57 32
BsmI GAATG_Cn'
Cuts at: 0 590 2121
Size: 590 1531
BsmBI CGTCTCn'nnnn_
Cuts at: 0 521 593 2121
Size: 521 72 1528
Fragments arranged by size:
1528 521 72
Bsp1286I G_dGCh'C
Cuts at: 0 32 89 524 560 1286 1657 1792 1848
Size: 32 57 435 36 726 371 135 56
Cuts at: 1848 2113 2121
Size: 265 8
Fragments arranged by size:
726 435 371 265 135 57 56 36
32 8
BspEI T'CCGG_A
Cuts at: 0 1850 2121
Size: 1850 271
BsrBI CCG'CTC
Cuts at: 0 459 716 1722 2121
Size: 459 257 1006 399
Fragments arranged by size:
1006 459 399 257
BsrDI GCAATG_nn'
Cuts at: 0 849 2121
Size: 849 1272
BsrFI r'CCGG_y
Cuts at: 0 247 797 1643 2121
Size: 247 550 846 478
Fragments arranged by size:
846 550 478 247
BsrGI T'GTAC_A
Cuts at: 0 476 1766 2121
Size: 476 1290 355
Fragments arranged by size:
1290 476 355
BssSI C'ACGA_G
Cuts at: 0 1189 2121
Size: 1189 932
BstEII G'GTnAC_C
Cuts at: 0 1836 2121
Size: 1836 285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1947 2121
Size: 1947 174
BstYI r'GATC_y
Cuts at: 0 371 1926 2121
Size: 371 1555 195
Fragments arranged by size:
1555 371 195
Bsu36I CC'TnA_GG
Cuts at: 0 2114 2121
Size: 2114 7
BtgI C'CryG_G
Cuts at: 0 289 397 773 908 1095 1351 1382 2121
Size: 289 108 376 135 187 256 31 739
Fragments arranged by size:
739 376 289 256 187 135 108 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1554 2121
Size: 1554 567
BtsI GCAGTG_nn'
Cuts at: 0 1490 2113 2121
Size: 1490 623 8
Fragments arranged by size:
1490 623 8
EaeI y'GGCC_r
Cuts at: 0 776 1209 1508 1645 2121
Size: 776 433 299 137 476
Fragments arranged by size:
776 476 433 299 137
EagI C'GGCC_G
Cuts at: 0 1645 2121
Size: 1645 476
EarI CTCTTCn'nnn_
Cuts at: 0 94 979 2121
Size: 94 885 1142
Fragments arranged by size:
1142 885 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 888 2121
Size: 888 1233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 205 292 1917 2121
Size: 116 89 87 1625 204
Fragments arranged by size:
1625 204 116 89 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 1063 2121
Size: 637 426 1058
Fragments arranged by size:
1058 637 426
EcoO109I rG'GnC_Cy
Cuts at: 0 202 300 1282 1283 1459 1483 1653 1654
Size: 202 98 982 1 176 24 170 1
Cuts at: 1654 1844 1845 2121
Size: 190 1 276
Fragments arranged by size:
982 276 202 190 176 170 98 24
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1173 1755 2121
Size: 1173 582 366
Fragments arranged by size:
1173 582 366
HaeII r_GCGC'y
Cuts at: 0 72 266 1110 1252 1440 1755 1893 1940
Size: 72 194 844 142 188 315 138 47
Cuts at: 1940 2121
Size: 181
Fragments arranged by size:
844 315 194 188 181 142 138 72
47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 178 210 613 645 730 762
Size: 96 32 50 32 403 32 85 32
Cuts at: 762 904 936 1219 1251 1911 1943 2121
Size: 142 32 283 32 660 32 178
Fragments arranged by size:
660 403 283 178 142 96 85 50
32 32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1732 2121
Size: 1732 389
KasI G'GCGC_C
Cuts at: 0 1106 1751 1889 2121
Size: 1106 645 138 232
Fragments arranged by size:
1106 645 232 138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 773 947 1384 2121
Size: 679 94 174 437 737
Fragments arranged by size:
737 679 437 174 94
MslI CAynn'nnrTG
Cuts at: 0 82 166 472 1325 1516 2121
Size: 82 84 306 853 191 605
Fragments arranged by size:
853 605 306 191 84 82
MspA1I CmG'CkG
Cuts at: 0 129 399 935 1097 1447 1471 1590 1883
Size: 129 270 536 162 350 24 119 293
Cuts at: 1883 2121
Size: 238
Fragments arranged by size:
536 350 293 270 238 162 129 119
24
NaeI GCC'GGC
Cuts at: 0 1645 2121
Size: 1645 476
NarI GG'CG_CC
Cuts at: 0 1107 1752 1890 2121
Size: 1107 645 138 231
Fragments arranged by size:
1107 645 231 138
NgoMIV G'CCGG_C
Cuts at: 0 1643 2121
Size: 1643 478
PasI CC'CwG_GG
Cuts at: 0 633 2121
Size: 633 1488
PflMI CCAn_nnn'nTGG
Cuts at: 0 1218 2121
Size: 1218 903
PfoI T'CCnGG_A
Cuts at: 0 2050 2121
Size: 2050 71
PpuMI rG'GwC_Cy
Cuts at: 0 300 1459 1483 2121
Size: 300 1159 24 638
Fragments arranged by size:
1159 638 300 24
PspOMI G'GGCC_C
Cuts at: 0 1282 1653 1788 1844 2121
Size: 1282 371 135 56 277
Fragments arranged by size:
1282 371 277 135 56
PstI C_TGCA'G
Cuts at: 0 2108 2121
Size: 2108 13
PvuII CAG'CTG
Cuts at: 0 129 1447 1471 1590 2121
Size: 129 1318 24 119 531
Fragments arranged by size:
1318 531 129 119 24
SacII CC_GC'GG
Cuts at: 0 400 1098 2121
Size: 400 698 1023
Fragments arranged by size:
1023 698 400
SanDI GG'GwC_CC
Cuts at: 0 300 2121
Size: 300 1821
SapI GCTCTTCn'nnn_
Cuts at: 0 94 979 2121
Size: 94 885 1142
Fragments arranged by size:
1142 885 94
ScaI AGT'ACT
Cuts at: 0 1072 1615 2121
Size: 1072 543 506
Fragments arranged by size:
1072 543 506
SexAI A'CCwGG_T
Cuts at: 0 2066 2121
Size: 2066 55
SfcI C'TryA_G
Cuts at: 0 2104 2121
Size: 2104 17
SfoI GGC'GCC
Cuts at: 0 1108 1753 1891 2121
Size: 1108 645 138 230
Fragments arranged by size:
1108 645 230 138
SmaI CCC'GGG
Cuts at: 0 835 1965 2121
Size: 835 1130 156
Fragments arranged by size:
1130 835 156
SmlI C'TyrA_G
Cuts at: 0 340 2121
Size: 340 1781
StyI C'CwwG_G
Cuts at: 0 882 1170 2121
Size: 882 288 951
Fragments arranged by size:
951 882 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1695 1752 2121
Size: 1695 57 369
Fragments arranged by size:
1695 369 57
TatI w'GTAC_w
Cuts at: 0 476 1070 1613 1766 2121
Size: 476 594 543 153 355
Fragments arranged by size:
594 543 476 355 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1811 1843 2121
Size: 1811 32 278
Fragments arranged by size:
1811 278 32
Tth111I GACn'n_nGTC
Cuts at: 0 496 1705 2121
Size: 496 1209 416
Fragments arranged by size:
1209 496 416
XmaI C'CCGG_G
Cuts at: 0 833 1963 2121
Size: 833 1130 158
Fragments arranged by size:
1130 833 158
XmnI GAAnn'nnTTC
Cuts at: 0 1558 1735 2121
Size: 1558 177 386
Fragments arranged by size:
1558 386 177
ZraI GAC'GTC
Cuts at: 0 1989 2121
Size: 1989 132
Enzymes that do cut:
AatII AcuI AgeI AleI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HindIII KasI MmeI MslI MspA1I
NaeI NarI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacII SanDI SapI ScaI SexAI SfcI
SfoI SmaI SmlI StyI TaqII TatI TstI Tth111I
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AhdI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BclI BfrBI BglII BlpI BmrI BmtI BsaBI BsiWI
BspHI BspMI BssHII BstAPI BstBI BstZ17I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SbfI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI XbaI XcmI
XhoI