Restriction map for
pF1KB9970
(Linear) (Six-base) MAPSORT of: KIBB9970.seq Check: 8138 from: 1 to: 2109
KIBB9970.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1095 2109
Size: 1095 1014
AccI GT'mk_AC
Cuts at: 0 1370 2109
Size: 1370 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 2041 2109
Size: 1856 185 68
Fragments arranged by size:
1856 185 68
AfeI AGC'GCT
Cuts at: 0 1213 2109
Size: 1213 896
AflIII A'CryG_T
Cuts at: 0 995 2109
Size: 995 1114
AleI CACnn'nnGTG
Cuts at: 0 313 1762 2109
Size: 313 1449 347
Fragments arranged by size:
1449 347 313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 205 2109
Size: 205 1904
AlwNI CAG_nnn'CTG
Cuts at: 0 621 740 1346 2109
Size: 621 119 606 763
Fragments arranged by size:
763 621 606 119
ApaI G_GGCC'C
Cuts at: 0 554 2005 2109
Size: 554 1451 104
Fragments arranged by size:
1451 554 104
ApaLI G'TGCA_C
Cuts at: 0 1777 2109
Size: 1777 332
AvaI C'yCGr_G
Cuts at: 0 1219 2109
Size: 1219 890
BamHI G'GATC_C
Cuts at: 0 850 2109
Size: 850 1259
BanI G'GyrC_C
Cuts at: 0 1223 2109
Size: 1223 886
BanII G_rGCy'C
Cuts at: 0 554 715 761 991 1097 1261 2005 2109
Size: 554 161 46 230 106 164 744 104
Fragments arranged by size:
744 554 230 164 161 106 104 46
BbeI G_GCGC'C
Cuts at: 0 1227 2109
Size: 1227 882
BbsI GAAGACnn'nnnn_
Cuts at: 0 408 1325 1445 1717 2109
Size: 408 917 120 272 392
Fragments arranged by size:
917 408 392 272 120
BfrBI ATG'CAT
Cuts at: 0 659 974 2109
Size: 659 315 1135
Fragments arranged by size:
1135 659 315
BglI GCCn_nnn'nGGC
Cuts at: 0 545 1232 1400 1885 2109
Size: 545 687 168 485 224
Fragments arranged by size:
687 545 485 224 168
BlpI GC'TnA_GC
Cuts at: 0 1091 1411 2109
Size: 1091 320 698
Fragments arranged by size:
1091 698 320
Bme1580I G_kGCm'C
Cuts at: 0 554 951 958 1016 1669 1781 2005 2109
Size: 554 397 7 58 653 112 224 104
Fragments arranged by size:
653 554 397 224 112 104 58 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 238 322 1684 2047 2109
Size: 118 120 84 1362 363 62
Fragments arranged by size:
1362 363 120 118 84 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 1612 2061 2109
Size: 439 1173 449 48
Fragments arranged by size:
1173 449 439 48
BsaAI yAC'GTr
Cuts at: 0 998 2109
Size: 998 1111
BsaHI Gr'CG_yC
Cuts at: 0 1224 2109
Size: 1224 885
BsaWI w'CCGG_w
Cuts at: 0 122 448 2109
Size: 122 326 1661
Fragments arranged by size:
1661 326 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1465 1495 1591 1621 1669 1699 1771 1801
Size: 1465 30 96 30 48 30 72 30
Cuts at: 1801 2109
Size: 308
Fragments arranged by size:
1465 308 96 72 48 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 517 557 1060 2109
Size: 235 282 40 503 1049
Fragments arranged by size:
1049 503 282 235 40
BseYI C'CCAG_C
Cuts at: 0 342 1254 2109
Size: 342 912 855
Fragments arranged by size:
912 855 342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1353 1539 2109
Size: 211 1142 186 570
Fragments arranged by size:
1142 570 211 186
BsiEI CG_ry'CG
Cuts at: 0 74 2109
Size: 74 2035
BsiHKAI G_wGCw'C
Cuts at: 0 761 1781 2109
Size: 761 1020 328
Fragments arranged by size:
1020 761 328
BsmI GAATG_Cn'
Cuts at: 0 184 661 1153 2109
Size: 184 477 492 956
Fragments arranged by size:
956 492 477 184
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2097 2109
Size: 2097 12
Bsp1286I G_dGCh'C
Cuts at: 0 554 715 761 951 958 991 1016 1097
Size: 554 161 46 190 7 33 25 81
Cuts at: 1097 1261 1669 1781 2005 2109
Size: 164 408 112 224 104
Fragments arranged by size:
554 408 224 190 164 161 112 104
81 46 33 25 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1095 1298 1630 1928 2109
Size: 1095 203 332 298 181
Fragments arranged by size:
1095 332 298 203 181
BsrBI CCG'CTC
Cuts at: 0 1685 2109
Size: 1685 424
BsrDI GCAATG_nn'
Cuts at: 0 330 2109
Size: 330 1779
BsrFI r'CCGG_y
Cuts at: 0 680 1394 2109
Size: 680 714 715
Fragments arranged by size:
715 714 680
BssHII G'CGCG_C
Cuts at: 0 6 1079 2109
Size: 6 1073 1030
Fragments arranged by size:
1073 1030 6
BstYI r'GATC_y
Cuts at: 0 850 2109
Size: 850 1259
BtgI C'CryG_G
Cuts at: 0 236 282 1026 1280 1967 2109
Size: 236 46 744 254 687 142
Fragments arranged by size:
744 687 254 236 142 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1096 1430 2109
Size: 1096 334 679
Fragments arranged by size:
1096 679 334
BtsI GCAGTG_nn'
Cuts at: 0 205 571 2109
Size: 205 366 1538
Fragments arranged by size:
1538 366 205
DrdI GACnn_nn'nnGTC
Cuts at: 0 88 589 2109
Size: 88 501 1520
Fragments arranged by size:
1520 501 88
EaeI y'GGCC_r
Cuts at: 0 71 128 251 1023 1233 1557 2109
Size: 71 57 123 772 210 324 552
Fragments arranged by size:
772 552 324 210 123 71 57
EagI C'GGCC_G
Cuts at: 0 71 2109
Size: 71 2038
EarI CTCTTCn'nnn_
Cuts at: 0 313 1832 2109
Size: 313 1519 277
Fragments arranged by size:
1519 313 277
EcoICRI GAG'CTC
Cuts at: 0 759 2109
Size: 759 1350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 238 322 1684 1856 2041 2047 2109
Size: 118 120 84 1362 172 185 6 62
Fragments arranged by size:
1362 185 172 120 118 84 62 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 2109
Size: 97 2012
EcoO109I rG'GnC_Cy
Cuts at: 0 27 468 516 551 2109
Size: 27 441 48 35 1558
Fragments arranged by size:
1558 441 48 35 27
EcoRV GAT'ATC
Cuts at: 0 924 2109
Size: 924 1185
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 906 2109
Size: 906 1203
HaeII r_GCGC'y
Cuts at: 0 1215 1227 2109
Size: 1215 12 882
Fragments arranged by size:
1215 882 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 149 619 651 1294 1326 2109
Size: 117 32 470 32 643 32 783
Fragments arranged by size:
783 643 470 117 32 32 32
HincII GTy'rAC
Cuts at: 0 1200 2076 2109
Size: 1200 876 33
Fragments arranged by size:
1200 876 33
HindIII A'AGCT_T
Cuts at: 0 1509 2109
Size: 1509 600
KasI G'GCGC_C
Cuts at: 0 1223 2109
Size: 1223 886
MscI TGG'CCA
Cuts at: 0 130 253 1235 2109
Size: 130 123 982 874
Fragments arranged by size:
982 874 130 123
MslI CAynn'nnrTG
Cuts at: 0 313 1762 1966 2066 2109
Size: 313 1449 204 100 43
Fragments arranged by size:
1449 313 204 100 43
MspA1I CmG'CkG
Cuts at: 0 39 216 269 536 618 677 722 729
Size: 39 177 53 267 82 59 45 7
Cuts at: 729 740 860 1142 1274 1490 1523 1649 1727
Size: 11 120 282 132 216 33 126 78
Cuts at: 1727 2109
Size: 382
Fragments arranged by size:
382 282 267 216 177 132 126 120
82 78 59 53 45 39 33 11
7
NaeI GCC'GGC
Cuts at: 0 682 1396 2109
Size: 682 714 713
Fragments arranged by size:
714 713 682
NarI GG'CG_CC
Cuts at: 0 1224 2109
Size: 1224 885
NdeI CA'TA_TG
Cuts at: 0 159 2109
Size: 159 1950
NgoMIV G'CCGG_C
Cuts at: 0 680 1394 2109
Size: 680 714 715
Fragments arranged by size:
715 714 680
NsiI A_TGCA'T
Cuts at: 0 661 976 2109
Size: 661 315 1133
Fragments arranged by size:
1133 661 315
NspI r_CATG'y
Cuts at: 0 428 974 1763 2109
Size: 428 546 789 346
Fragments arranged by size:
789 546 428 346
PasI CC'CwG_GG
Cuts at: 0 363 2109
Size: 363 1746
PfoI T'CCnGG_A
Cuts at: 0 95 2109
Size: 95 2014
PmlI CAC'GTG
Cuts at: 0 998 2109
Size: 998 1111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2089 2109
Size: 2089 20
PpuMI rG'GwC_Cy
Cuts at: 0 27 516 2109
Size: 27 489 1593
Fragments arranged by size:
1593 489 27
PspOMI G'GGCC_C
Cuts at: 0 550 2001 2109
Size: 550 1451 108
Fragments arranged by size:
1451 550 108
PstI C_TGCA'G
Cuts at: 0 1273 2109
Size: 1273 836
PvuII CAG'CTG
Cuts at: 0 216 618 677 740 1274 1523 2109
Size: 216 402 59 63 534 249 586
Fragments arranged by size:
586 534 402 249 216 63 59
RsrII CG'GwC_CG
Cuts at: 0 1722 2109
Size: 1722 387
SacI G_AGCT'C
Cuts at: 0 761 2109
Size: 761 1348
SanDI GG'GwC_CC
Cuts at: 0 27 2109
Size: 27 2082
ScaI AGT'ACT
Cuts at: 0 2101 2109
Size: 2101 8
SfcI C'TryA_G
Cuts at: 0 874 1269 1347 1539 2109
Size: 874 395 78 192 570
Fragments arranged by size:
874 570 395 192 78
SfoI GGC'GCC
Cuts at: 0 1225 2109
Size: 1225 884
SmaI CCC'GGG
Cuts at: 0 1221 2109
Size: 1221 888
SphI G_CATG'C
Cuts at: 0 428 2109
Size: 428 1681
StuI AGG'CCT
Cuts at: 0 103 2109
Size: 103 2006
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1960 2109
Size: 1960 149
TatI w'GTAC_w
Cuts at: 0 380 2099 2109
Size: 380 1719 10
Fragments arranged by size:
1719 380 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1465 1497 1591 1623 1771 1803 2109
Size: 1465 32 94 32 148 32 306
Fragments arranged by size:
1465 306 148 94 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1720 2109
Size: 1720 389
XmaI C'CCGG_G
Cuts at: 0 1219 2109
Size: 1219 890
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BfrBI BglI BlpI Bme1580I BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstYI
BtgI BtgZI BtsI DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII KasI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PasI PfoI PmlI PpiI PpuMI
PspOMI PstI PvuII RsrII SacI SanDI ScaI SfcI
SfoI SmaI SphI StuI TaqII TatI TstI Tth111I
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BclI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsiWI BspEI BspHI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII
EciI EcoRI FseI FspI FspAI HpaI KpnI MfeI
MluI MmeI NcoI NheI NotI NruI PacI PciI
PflMI PmeI PshAI PsiI PspXI PsrI PvuI SacII
SalI SapI SbfI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StyI SwaI XbaI XcmI
XhoI XmnI ZraI