Restriction map for
pF1KB9965
(Linear) (Six-base) MAPSORT of: KIBB9965.seq Check: 4337 from: 1 to: 1647
KIBB9965.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 298 1647
Size: 298 1349
Acc65I G'GTAC_C
Cuts at: 0 199 1647
Size: 199 1448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 805 1436 1647
Size: 395 410 631 211
Fragments arranged by size:
631 410 395 211
AfeI AGC'GCT
Cuts at: 0 1135 1647
Size: 1135 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1154 1304 1647
Size: 1154 150 343
Fragments arranged by size:
1154 343 150
ApaLI G'TGCA_C
Cuts at: 0 1139 1647
Size: 1139 508
ApoI r'AATT_y
Cuts at: 0 394 451 788 1257 1405 1647
Size: 394 57 337 469 148 242
Fragments arranged by size:
469 394 337 242 148 57
BanI G'GyrC_C
Cuts at: 0 79 199 1647
Size: 79 120 1448
Fragments arranged by size:
1448 120 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 1363 1462 1647
Size: 1363 99 185
Fragments arranged by size:
1363 185 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1204 1238 1647
Size: 1204 34 409
Fragments arranged by size:
1204 409 34
BglI GCCn_nnn'nGGC
Cuts at: 0 95 1647
Size: 95 1552
BlpI GC'TnA_GC
Cuts at: 0 433 725 1647
Size: 433 292 922
Fragments arranged by size:
922 433 292
Bme1580I G_kGCm'C
Cuts at: 0 82 494 1143 1647
Size: 82 412 649 504
Fragments arranged by size:
649 504 412 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 205 550 1385 1647
Size: 205 345 835 262
Fragments arranged by size:
835 345 262 205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1647
Size: 1006 641
Bpu10I CC'TnA_GC
Cuts at: 0 386 1647
Size: 386 1261
BsaHI Gr'CG_yC
Cuts at: 0 167 1647
Size: 167 1480
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 1647
Size: 127 30 1490
Fragments arranged by size:
1490 127 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 151 648 1647
Size: 139 12 497 999
Fragments arranged by size:
999 497 139 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1647
Size: 34 1613
BsiHKAI G_wGCw'C
Cuts at: 0 1143 1647
Size: 1143 504
BsmI GAATG_Cn'
Cuts at: 0 268 372 1647
Size: 268 104 1275
Fragments arranged by size:
1275 268 104
Bsp1286I G_dGCh'C
Cuts at: 0 82 494 1143 1647
Size: 82 412 649 504
Fragments arranged by size:
649 504 412 82
BsrFI r'CCGG_y
Cuts at: 0 53 1647
Size: 53 1594
BstAPI GCAn_nnn'nTGC
Cuts at: 0 86 1647
Size: 86 1561
BstBI TT'CG_AA
Cuts at: 0 228 1596 1647
Size: 228 1368 51
Fragments arranged by size:
1368 228 51
BstEII G'GTnAC_C
Cuts at: 0 1026 1117 1647
Size: 1026 91 530
Fragments arranged by size:
1026 530 91
BstYI r'GATC_y
Cuts at: 0 1217 1507 1647
Size: 1217 290 140
Fragments arranged by size:
1217 290 140
Bsu36I CC'TnA_GG
Cuts at: 0 673 1647
Size: 673 974
BtgI C'CryG_G
Cuts at: 0 28 1647
Size: 28 1619
ClaI AT'CG_AT
Cuts at: 0 503 1647
Size: 503 1144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 729 764 1647
Size: 729 35 883
Fragments arranged by size:
883 729 35
DraIII CAC_nnn'GTG
Cuts at: 0 86 1647
Size: 86 1561
EarI CTCTTCn'nnn_
Cuts at: 0 521 1201 1647
Size: 521 680 446
Fragments arranged by size:
680 521 446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 805 1006 1436 1647
Size: 395 410 201 430 211
Fragments arranged by size:
430 410 395 211 201
EcoO109I rG'GnC_Cy
Cuts at: 0 1086 1647
Size: 1086 561
EcoRI G'AATT_C
Cuts at: 0 788 1647
Size: 788 859
HaeII r_GCGC'y
Cuts at: 0 552 1137 1647
Size: 552 585 510
Fragments arranged by size:
585 552 510
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 694 726 1209 1241 1647
Size: 280 32 382 32 483 32 406
Fragments arranged by size:
483 406 382 280 32 32 32
HincII GTy'rAC
Cuts at: 0 1548 1647
Size: 1548 99
HindIII A'AGCT_T
Cuts at: 0 848 1647
Size: 848 799
HpaI GTT'AAC
Cuts at: 0 1548 1647
Size: 1548 99
KpnI G_GTAC'C
Cuts at: 0 203 1647
Size: 203 1444
MfeI C'AATT_G
Cuts at: 0 479 1647
Size: 479 1168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 1480 1647
Size: 221 1259 167
Fragments arranged by size:
1259 221 167
MslI CAynn'nnrTG
Cuts at: 0 319 1286 1647
Size: 319 967 361
Fragments arranged by size:
967 361 319
MspA1I CmG'CkG
Cuts at: 0 30 1647
Size: 30 1617
NdeI CA'TA_TG
Cuts at: 0 1533 1647
Size: 1533 114
NspI r_CATG'y
Cuts at: 0 1285 1647
Size: 1285 362
PflMI CCAn_nnn'nTGG
Cuts at: 0 425 619 1647
Size: 425 194 1028
Fragments arranged by size:
1028 425 194
PfoI T'CCnGG_A
Cuts at: 0 249 1647
Size: 249 1398
PstI C_TGCA'G
Cuts at: 0 1112 1647
Size: 1112 535
SacII CC_GC'GG
Cuts at: 0 31 1647
Size: 31 1616
SapI GCTCTTCn'nnn_
Cuts at: 0 521 1647
Size: 521 1126
ScaI AGT'ACT
Cuts at: 0 560 1647
Size: 560 1087
SfcI C'TryA_G
Cuts at: 0 1108 1348 1647
Size: 1108 240 299
Fragments arranged by size:
1108 299 240
SphI G_CATG'C
Cuts at: 0 1285 1647
Size: 1285 362
SspI AAT'ATT
Cuts at: 0 986 1647
Size: 986 661
StuI AGG'CCT
Cuts at: 0 901 1647
Size: 901 746
StyI C'CwwG_G
Cuts at: 0 1034 1647
Size: 1034 613
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 893 1136 1647
Size: 893 243 511
Fragments arranged by size:
893 511 243
TatI w'GTAC_w
Cuts at: 0 558 1647
Size: 558 1089
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 1059 1091 1195 1227 1647
Size: 127 32 900 32 104 32 420
Fragments arranged by size:
900 420 127 104 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1362 1647
Size: 1362 285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 580 1647
Size: 580 1067
XmnI GAAnn'nnTTC
Cuts at: 0 515 1011 1647
Size: 515 496 636
Fragments arranged by size:
636 515 496
Enzymes that do cut:
AccI Acc65I AcuI AfeI AlfI ApaLI ApoI BanI
BbsI BcgI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrFI
BstAPI BstBI BstEII BstYI Bsu36I BtgI ClaI CspCI
DraIII EarI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MmeI MslI MspA1I NdeI
NspI PflMI PfoI PstI SacII SapI ScaI SfcI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BciVI BclI BfrBI
BglII BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstXI BstZ17I BtgZI
BtsI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI KasI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SwaI XbaI XhoI
XmaI ZraI