Restriction map for
pF1KB9960
(Linear) (Six-base) MAPSORT of: KIBB9960.seq Check: 6659 from: 1 to: 2067
KIBB9960.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 872 2067
Size: 872 1195
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 490 979 1433 1615 1893 2067
Size: 196 294 489 454 182 278 174
Fragments arranged by size:
489 454 294 278 196 182 174
AflIII A'CryG_T
Cuts at: 0 512 2067
Size: 512 1555
AleI CACnn'nnGTG
Cuts at: 0 250 2067
Size: 250 1817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 2067
Size: 114 1953
AlwNI CAG_nnn'CTG
Cuts at: 0 57 2067
Size: 57 2010
ApoI r'AATT_y
Cuts at: 0 922 1336 1532 2067
Size: 922 414 196 535
Fragments arranged by size:
922 535 414 196
AseI AT'TA_AT
Cuts at: 0 362 1198 2067
Size: 362 836 869
Fragments arranged by size:
869 836 362
BanII G_rGCy'C
Cuts at: 0 488 608 2067
Size: 488 120 1459
Fragments arranged by size:
1459 488 120
BciVI GTATCCnnnnn_n'
Cuts at: 0 234 2067
Size: 234 1833
BclI T'GATC_A
Cuts at: 0 519 1137 1350 2067
Size: 519 618 213 717
Fragments arranged by size:
717 618 519 213
BfrBI ATG'CAT
Cuts at: 0 886 2067
Size: 886 1181
BglII A'GATC_T
Cuts at: 0 1281 2067
Size: 1281 786
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1232 2067
Size: 1232 835
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 1166 2067
Size: 970 196 901
Fragments arranged by size:
970 901 196
Bpu10I CC'TnA_GC
Cuts at: 0 1703 2067
Size: 1703 364
BsaI GGTCTCn'nnnn_
Cuts at: 0 1142 2067
Size: 1142 925
BsaAI yAC'GTr
Cuts at: 0 1072 2067
Size: 1072 995
BsaBI GATnn'nnATC
Cuts at: 0 1355 2067
Size: 1355 712
BsaWI w'CCGG_w
Cuts at: 0 1321 2067
Size: 1321 746
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1472 1502 2067
Size: 1472 30 565
Fragments arranged by size:
1472 565 30
BseYI C'CCAG_C
Cuts at: 0 27 1756 2067
Size: 27 1729 311
Fragments arranged by size:
1729 311 27
BsiHKAI G_wGCw'C
Cuts at: 0 488 2067
Size: 488 1579
Bsp1286I G_dGCh'C
Cuts at: 0 488 608 2067
Size: 488 120 1459
Fragments arranged by size:
1459 488 120
BsrGI T'GTAC_A
Cuts at: 0 833 2067
Size: 833 1234
BstEII G'GTnAC_C
Cuts at: 0 595 2067
Size: 595 1472
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1306 2067
Size: 1306 761
BstYI r'GATC_y
Cuts at: 0 1281 1589 2067
Size: 1281 308 478
Fragments arranged by size:
1281 478 308
BstZ17I GTA'TAC
Cuts at: 0 873 2067
Size: 873 1194
BtsI GCAGTG_nn'
Cuts at: 0 1578 2040 2067
Size: 1578 462 27
Fragments arranged by size:
1578 462 27
DraI TTT'AAA
Cuts at: 0 1157 1316 2067
Size: 1157 159 751
Fragments arranged by size:
1157 751 159
EaeI y'GGCC_r
Cuts at: 0 48 347 1505 1711 2067
Size: 48 299 1158 206 356
Fragments arranged by size:
1158 356 299 206 48
EarI CTCTTCn'nnn_
Cuts at: 0 650 1580 2067
Size: 650 930 487
Fragments arranged by size:
930 650 487
EcoICRI GAG'CTC
Cuts at: 0 486 2067
Size: 486 1581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 490 970 979 1166 1433 1615 1893
Size: 196 294 480 9 187 267 182 278
Cuts at: 1893 2067
Size: 174
Fragments arranged by size:
480 294 278 267 196 187 182 174
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 2067
Size: 130 1937
EcoO109I rG'GnC_Cy
Cuts at: 0 1224 1437 1929 2067
Size: 1224 213 492 138
Fragments arranged by size:
1224 492 213 138
EcoRV GAT'ATC
Cuts at: 0 1662 2067
Size: 1662 405
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 1574 1606 1651 1683 2067
Size: 658 32 884 32 45 32 384
Fragments arranged by size:
884 658 384 45 32 32 32
MscI TGG'CCA
Cuts at: 0 50 349 1507 1713 2067
Size: 50 299 1158 206 354
Fragments arranged by size:
1158 354 299 206 50
MslI CAynn'nnrTG
Cuts at: 0 250 866 2067
Size: 250 616 1201
Fragments arranged by size:
1201 616 250
MspA1I CmG'CkG
Cuts at: 0 57 98 250 2067
Size: 57 41 152 1817
Fragments arranged by size:
1817 152 57 41
NdeI CA'TA_TG
Cuts at: 0 1264 2067
Size: 1264 803
NsiI A_TGCA'T
Cuts at: 0 888 2067
Size: 888 1179
NspI r_CATG'y
Cuts at: 0 516 2067
Size: 516 1551
PciI A'CATG_T
Cuts at: 0 512 2067
Size: 512 1555
PflMI CCAn_nnn'nTGG
Cuts at: 0 1497 2067
Size: 1497 570
PpuMI rG'GwC_Cy
Cuts at: 0 1224 1437 2067
Size: 1224 213 630
Fragments arranged by size:
1224 630 213
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2 34 392 424 2067
Size: 2 32 358 32 1643
Fragments arranged by size:
1643 358 32 32 2
PstI C_TGCA'G
Cuts at: 0 103 2067
Size: 103 1964
PvuII CAG'CTG
Cuts at: 0 98 250 2067
Size: 98 152 1817
Fragments arranged by size:
1817 152 98
SacI G_AGCT'C
Cuts at: 0 488 2067
Size: 488 1579
ScaI AGT'ACT
Cuts at: 0 430 2067
Size: 430 1637
SfcI C'TryA_G
Cuts at: 0 99 2067
Size: 99 1968
SspI AAT'ATT
Cuts at: 0 379 2067
Size: 379 1688
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1634 1951 2067
Size: 1634 317 116
Fragments arranged by size:
1634 317 116
TatI w'GTAC_w
Cuts at: 0 428 833 1302 2067
Size: 428 405 469 765
Fragments arranged by size:
765 469 428 405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 530 562 568 600 2067
Size: 530 32 6 32 1467
Fragments arranged by size:
1467 530 32 32 6
XbaI T'CTAG_A
Cuts at: 0 1284 2067
Size: 1284 783
XmnI GAAnn'nnTTC
Cuts at: 0 741 1523 2067
Size: 741 782 544
Fragments arranged by size:
782 741 544
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI
BanII BciVI BclI BfrBI BglII BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsiHKAI Bsp1286I
BsrGI BstEII BstXI BstYI BstZ17I BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I MscI
MslI MspA1I NdeI NsiI NspI PciI PflMI PpuMI
PsrI PstI PvuII SacI ScaI SfcI SspI TaqII
TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BmtI BpuEI BsaHI BseRI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoRI
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI StyI SwaI Tth111I XcmI XhoI XmaI ZraI