Restriction map for
pF1KB9959
(Linear) (Six-base) MAPSORT of: KIBB9959.seq Check: 3361 from: 1 to: 1614
KIBB9959.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 173 234 579 718 1461 1614
Size: 173 61 345 139 743 153
Fragments arranged by size:
743 345 173 153 139 61
AatII G_ACGT'C
Cuts at: 0 854 1614
Size: 854 760
Acc65I G'GTAC_C
Cuts at: 0 815 1614
Size: 815 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1614
Size: 803 811
AleI CACnn'nnGTG
Cuts at: 0 673 1614
Size: 673 941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 949 981 1614
Size: 949 32 633
Fragments arranged by size:
949 633 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1170 1614
Size: 1170 444
AvaI C'yCGr_G
Cuts at: 0 5 118 136 681 1598 1614
Size: 5 113 18 545 917 16
Fragments arranged by size:
917 545 113 18 16 5
BanI G'GyrC_C
Cuts at: 0 142 675 815 901 1327 1437 1614
Size: 142 533 140 86 426 110 177
Fragments arranged by size:
533 426 177 142 140 110 86
BanII G_rGCy'C
Cuts at: 0 93 359 389 872 934 1614
Size: 93 266 30 483 62 680
Fragments arranged by size:
680 483 266 93 62 30
BbeI G_GCGC'C
Cuts at: 0 1331 1614
Size: 1331 283
BbsI GAAGACnn'nnnn_
Cuts at: 0 1199 1469 1614
Size: 1199 270 145
Fragments arranged by size:
1199 270 145
BbvCI CC'TCA_GC
Cuts at: 0 497 1614
Size: 497 1117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 30 64 1614
Size: 30 34 1550
Fragments arranged by size:
1550 34 30
BglI GCCn_nnn'nGGC
Cuts at: 0 373 1446 1614
Size: 373 1073 168
Fragments arranged by size:
1073 373 168
BlpI GC'TnA_GC
Cuts at: 0 1019 1614
Size: 1019 595
Bme1580I G_kGCm'C
Cuts at: 0 145 680 1614
Size: 145 535 934
Fragments arranged by size:
934 535 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 612 1614
Size: 344 268 1002
Fragments arranged by size:
1002 344 268
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 151 1278 1614
Size: 151 1127 336
Fragments arranged by size:
1127 336 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 906 1219 1264 1427 1614
Size: 343 563 313 45 163 187
Fragments arranged by size:
563 343 313 187 163 45
Bpu10I CC'TnA_GC
Cuts at: 0 497 841 1614
Size: 497 344 773
Fragments arranged by size:
773 497 344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1288 1614
Size: 1288 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 1036 1614
Size: 1036 578
BsaHI Gr'CG_yC
Cuts at: 0 43 365 851 1328 1614
Size: 43 322 486 477 286
Fragments arranged by size:
486 477 322 286 43
BsaWI w'CCGG_w
Cuts at: 0 85 1614
Size: 85 1529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 1495 1525 1614
Size: 142 30 1323 30 89
Fragments arranged by size:
1323 142 89 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1198 1504 1513 1516 1614
Size: 1198 306 9 3 98
Fragments arranged by size:
1198 306 98 9 3
BseYI C'CCAG_C
Cuts at: 0 271 280 935 1265 1614
Size: 271 9 655 330 349
Fragments arranged by size:
655 349 330 271 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 1614
Size: 969 645
BsiEI CG_ry'CG
Cuts at: 0 825 1614
Size: 825 789
BsiHKAI G_wGCw'C
Cuts at: 0 334 359 892 1295 1614
Size: 334 25 533 403 319
Fragments arranged by size:
533 403 334 319 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 321 1614
Size: 321 1293
Bsp1286I G_dGCh'C
Cuts at: 0 93 145 334 359 389 680 872 892
Size: 93 52 189 25 30 291 192 20
Cuts at: 892 934 1295 1614
Size: 42 361 319
Fragments arranged by size:
361 319 291 192 189 93 52 42
30 25 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 234 579 718 1014 1461 1614
Size: 173 61 345 139 296 447 153
Fragments arranged by size:
447 345 296 173 153 139 61
BsrBI CCG'CTC
Cuts at: 0 303 1614
Size: 303 1311
BsrDI GCAATG_nn'
Cuts at: 0 1106 1614
Size: 1106 508
BsrFI r'CCGG_y
Cuts at: 0 1145 1614
Size: 1145 469
BsrGI T'GTAC_A
Cuts at: 0 188 1614
Size: 188 1426
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1170 1350 1614
Size: 1170 180 264
Fragments arranged by size:
1170 264 180
BstEII G'GTnAC_C
Cuts at: 0 201 705 1614
Size: 201 504 909
Fragments arranged by size:
909 504 201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1002 1419 1614
Size: 1002 417 195
Fragments arranged by size:
1002 417 195
BstYI r'GATC_y
Cuts at: 0 578 1476 1614
Size: 578 898 138
Fragments arranged by size:
898 578 138
Bsu36I CC'TnA_GG
Cuts at: 0 897 1614
Size: 897 717
BtgI C'CryG_G
Cuts at: 0 146 381 872 1001 1127 1614
Size: 146 235 491 129 126 487
Fragments arranged by size:
491 487 235 146 129 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1080 1614
Size: 1080 534
BtsI GCAGTG_nn'
Cuts at: 0 926 959 1614
Size: 926 33 655
Fragments arranged by size:
926 655 33
DraIII CAC_nnn'GTG
Cuts at: 0 954 1614
Size: 954 660
DrdI GACnn_nn'nnGTC
Cuts at: 0 624 1614
Size: 624 990
EaeI y'GGCC_r
Cuts at: 0 55 245 638 822 1614
Size: 55 190 393 184 792
Fragments arranged by size:
792 393 190 184 55
EagI C'GGCC_G
Cuts at: 0 822 1614
Size: 822 792
EarI CTCTTCn'nnn_
Cuts at: 0 1276 1502 1614
Size: 1276 226 112
Fragments arranged by size:
1276 226 112
EcoICRI GAG'CTC
Cuts at: 0 357 1614
Size: 357 1257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 803 906 1219 1264 1427 1614
Size: 343 460 103 313 45 163 187
Fragments arranged by size:
460 343 313 187 163 103 45
EcoO109I rG'GnC_Cy
Cuts at: 0 268 525 632 1204 1283 1393 1424 1614
Size: 268 257 107 572 79 110 31 190
Fragments arranged by size:
572 268 257 190 110 107 79 31
HaeII r_GCGC'y
Cuts at: 0 216 1331 1614
Size: 216 1115 283
Fragments arranged by size:
1115 283 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 292 324 1531 1563 1614
Size: 176 32 84 32 1207 32 51
Fragments arranged by size:
1207 176 84 51 32 32 32
KasI G'GCGC_C
Cuts at: 0 1327 1614
Size: 1327 287
KpnI G_GTAC'C
Cuts at: 0 819 1614
Size: 819 795
MslI CAynn'nnrTG
Cuts at: 0 239 673 1435 1609 1614
Size: 239 434 762 174 5
Fragments arranged by size:
762 434 239 174 5
MspA1I CmG'CkG
Cuts at: 0 13 48 60 939 1155 1614
Size: 13 35 12 879 216 459
Fragments arranged by size:
879 459 216 35 13 12
NaeI GCC'GGC
Cuts at: 0 1147 1614
Size: 1147 467
NarI GG'CG_CC
Cuts at: 0 1328 1614
Size: 1328 286
NgoMIV G'CCGG_C
Cuts at: 0 1145 1614
Size: 1145 469
PasI CC'CwG_GG
Cuts at: 0 927 1614
Size: 927 687
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 954 1614
Size: 243 711 660
Fragments arranged by size:
711 660 243
PfoI T'CCnGG_A
Cuts at: 0 77 180 359 1614
Size: 77 103 179 1255
Fragments arranged by size:
1255 179 103 77
PpuMI rG'GwC_Cy
Cuts at: 0 268 525 632 1204 1393 1424 1614
Size: 268 257 107 572 189 31 190
Fragments arranged by size:
572 268 257 190 189 107 31
PstI C_TGCA'G
Cuts at: 0 1175 1614
Size: 1175 439
PvuII CAG'CTG
Cuts at: 0 939 1614
Size: 939 675
SacI G_AGCT'C
Cuts at: 0 359 1614
Size: 359 1255
SanDI GG'GwC_CC
Cuts at: 0 268 632 1424 1614
Size: 268 364 792 190
Fragments arranged by size:
792 364 268 190
SapI GCTCTTCn'nnn_
Cuts at: 0 1276 1614
Size: 1276 338
SfcI C'TryA_G
Cuts at: 0 1171 1551 1614
Size: 1171 380 63
Fragments arranged by size:
1171 380 63
SfoI GGC'GCC
Cuts at: 0 1329 1614
Size: 1329 285
SmaI CCC'GGG
Cuts at: 0 7 1600 1614
Size: 7 1593 14
Fragments arranged by size:
1593 14 7
SmlI C'TyrA_G
Cuts at: 0 1303 1614
Size: 1303 311
SrfI GCCC'GGGC
Cuts at: 0 7 1614
Size: 7 1607
StyI C'CwwG_G
Cuts at: 0 437 671 1376 1614
Size: 437 234 705 238
Fragments arranged by size:
705 437 238 234
TatI w'GTAC_w
Cuts at: 0 188 1614
Size: 188 1426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 142 174 1614
Size: 142 32 1440
Fragments arranged by size:
1440 142 32
XbaI T'CTAG_A
Cuts at: 0 1479 1614
Size: 1479 135
XmaI C'CCGG_G
Cuts at: 0 5 1598 1614
Size: 5 1593 16
Fragments arranged by size:
1593 16 5
ZraI GAC'GTC
Cuts at: 0 852 1614
Size: 852 762
Enzymes that do cut:
AarI AatII Acc65I AcuI AleI AloI AlwNI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
KasI KpnI MslI MspA1I NaeI NarI NgoMIV PasI
PflMI PfoI PpuMI PstI PvuII SacI SanDI SapI
SfcI SfoI SmaI SmlI SrfI StyI TatI TstI
XbaI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AlfI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BmgBI BmtI BsaAI
BsaBI BsiWI BsmI BspEI BspHI BssHII BssSI BstBI
BstZ17I ClaI CspCI DraI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TaqII Tth111I XcmI XhoI
XmnI