Restriction map for
pF1KB9949
(Linear) (Six-base) MAPSORT of: KIBB9949.seq Check: 6632 from: 1 to: 1596
KIBB9949.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1040 1596
Size: 1040 556
AatII G_ACGT'C
Cuts at: 0 561 1596
Size: 561 1035
AccI GT'mk_AC
Cuts at: 0 1476 1596
Size: 1476 120
Acc65I G'GTAC_C
Cuts at: 0 1380 1596
Size: 1380 216
AclI AA'CG_TT
Cuts at: 0 1431 1596
Size: 1431 165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 818 1596
Size: 275 543 778
Fragments arranged by size:
778 543 275
AlwNI CAG_nnn'CTG
Cuts at: 0 75 1596
Size: 75 1521
ApaI G_GGCC'C
Cuts at: 0 1515 1596
Size: 1515 81
ApoI r'AATT_y
Cuts at: 0 1043 1156 1596
Size: 1043 113 440
Fragments arranged by size:
1043 440 113
AseI AT'TA_AT
Cuts at: 0 215 1596
Size: 215 1381
BanI G'GyrC_C
Cuts at: 0 1051 1207 1380 1596
Size: 1051 156 173 216
Fragments arranged by size:
1051 216 173 156
BanII G_rGCy'C
Cuts at: 0 990 1515 1596
Size: 990 525 81
Fragments arranged by size:
990 525 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 1561 1596
Size: 1561 35
BbvCI CC'TCA_GC
Cuts at: 0 5 1596
Size: 5 1591
BciVI GTATCCnnnnn_n'
Cuts at: 0 153 1596
Size: 153 1443
BclI T'GATC_A
Cuts at: 0 500 1596
Size: 500 1096
BfrBI ATG'CAT
Cuts at: 0 227 1596
Size: 227 1369
Bme1580I G_kGCm'C
Cuts at: 0 197 1190 1210 1515 1596
Size: 197 993 20 305 81
Fragments arranged by size:
993 305 197 81 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 107 1596
Size: 107 1489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1512 1596
Size: 495 1017 84
Fragments arranged by size:
1017 495 84
Bpu10I CC'TnA_GC
Cuts at: 0 5 147 189 1596
Size: 5 142 42 1407
Fragments arranged by size:
1407 142 42 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1596
Size: 534 1062
BsaI GGTCTCn'nnnn_
Cuts at: 0 1076 1596
Size: 1076 520
BsaHI Gr'CG_yC
Cuts at: 0 558 1596
Size: 558 1038
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1331 1361 1596
Size: 1331 30 235
Fragments arranged by size:
1331 235 30
BseYI C'CCAG_C
Cuts at: 0 347 1508 1596
Size: 347 1161 88
Fragments arranged by size:
1161 347 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1596
Size: 1018 578
BsmI GAATG_Cn'
Cuts at: 0 449 1510 1596
Size: 449 1061 86
Fragments arranged by size:
1061 449 86
Bsp1286I G_dGCh'C
Cuts at: 0 197 990 1190 1210 1515 1596
Size: 197 793 200 20 305 81
Fragments arranged by size:
793 305 200 197 81 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1040 1596
Size: 1040 556
BsrDI GCAATG_nn'
Cuts at: 0 240 1130 1596
Size: 240 890 466
Fragments arranged by size:
890 466 240
BssSI C'ACGA_G
Cuts at: 0 56 1596
Size: 56 1540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 940 1596
Size: 940 656
BstYI r'GATC_y
Cuts at: 0 313 943 1596
Size: 313 630 653
Fragments arranged by size:
653 630 313
BtsI GCAGTG_nn'
Cuts at: 0 409 1596
Size: 409 1187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 741 776 1596
Size: 741 35 820
Fragments arranged by size:
820 741 35
DraI TTT'AAA
Cuts at: 0 176 1596
Size: 176 1420
EaeI y'GGCC_r
Cuts at: 0 1438 1596
Size: 1438 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 495 818 1512 1596
Size: 275 220 323 694 84
Fragments arranged by size:
694 323 275 220 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1308 1340 1596
Size: 1308 32 256
Fragments arranged by size:
1308 256 32
KpnI G_GTAC'C
Cuts at: 0 1384 1596
Size: 1384 212
MfeI C'AATT_G
Cuts at: 0 1070 1360 1442 1596
Size: 1070 290 82 154
Fragments arranged by size:
1070 290 154 82
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 355 585 1596
Size: 140 215 230 1011
Fragments arranged by size:
1011 230 215 140
MslI CAynn'nnrTG
Cuts at: 0 100 475 938 1596
Size: 100 375 463 658
Fragments arranged by size:
658 463 375 100
MspA1I CmG'CkG
Cuts at: 0 515 809 1012 1596
Size: 515 294 203 584
Fragments arranged by size:
584 515 294 203
NsiI A_TGCA'T
Cuts at: 0 229 1596
Size: 229 1367
NspI r_CATG'y
Cuts at: 0 227 1596
Size: 227 1369
PfoI T'CCnGG_A
Cuts at: 0 901 1596
Size: 901 695
PspOMI G'GGCC_C
Cuts at: 0 1511 1596
Size: 1511 85
PvuII CAG'CTG
Cuts at: 0 515 809 1012 1596
Size: 515 294 203 584
Fragments arranged by size:
584 515 294 203
SfcI C'TryA_G
Cuts at: 0 1290 1596
Size: 1290 306
SmlI C'TyrA_G
Cuts at: 0 549 1596
Size: 549 1047
StyI C'CwwG_G
Cuts at: 0 1056 1596
Size: 1056 540
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1069 1596
Size: 1069 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1331 1363 1596
Size: 1331 32 233
Fragments arranged by size:
1331 233 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1218 1596
Size: 1218 378
XmnI GAAnn'nnTTC
Cuts at: 0 784 1596
Size: 784 812
ZraI GAC'GTC
Cuts at: 0 559 1596
Size: 559 1037
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AlwNI ApaI
ApoI AseI BanI BanII BbsI BbvCI BciVI BclI
BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseYI BsgI BsmI Bsp1286I BspMI BsrDI BssSI
BstXI BstYI BtsI CspCI DraI EaeI Eco57MI Hin4I
KpnI MfeI MmeI MslI MspA1I NsiI NspI PfoI
PspOMI PvuII SfcI SmlI StyI TaqII TstI XcmI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AflIII AgeI AhdI AleI AlfI AloI
ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BglI BglII BlpI BmgBI BmtI BplI BsaAI
BsaBI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI