Restriction map for
pF1KB9940
(Linear) (Six-base) MAPSORT of: KIBB9940.seq Check: 7339 from: 1 to: 1971
KIBB9940.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
Acc65I G'GTAC_C
Cuts at: 0 1157 1971
Size: 1157 814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 866 1971
Size: 820 46 1105
Fragments arranged by size:
1105 820 46
AflIII A'CryG_T
Cuts at: 0 936 1148 1971
Size: 936 212 823
Fragments arranged by size:
936 823 212
AlwNI CAG_nnn'CTG
Cuts at: 0 1228 1971
Size: 1228 743
ApaI G_GGCC'C
Cuts at: 0 864 1971
Size: 864 1107
ApaLI G'TGCA_C
Cuts at: 0 500 1421 1971
Size: 500 921 550
Fragments arranged by size:
921 550 500
ApoI r'AATT_y
Cuts at: 0 1127 1189 1263 1361 1843 1971
Size: 1127 62 74 98 482 128
Fragments arranged by size:
1127 482 128 98 74 62
AvrII C'CTAG_G
Cuts at: 0 1317 1971
Size: 1317 654
BamHI G'GATC_C
Cuts at: 0 285 1971
Size: 285 1686
BanI G'GyrC_C
Cuts at: 0 1157 1971
Size: 1157 814
BanII G_rGCy'C
Cuts at: 0 40 864 1834 1971
Size: 40 824 970 137
Fragments arranged by size:
970 824 137 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 854 972 996 1971
Size: 854 118 24 975
Fragments arranged by size:
975 854 118 24
BbvCI CC'TCA_GC
Cuts at: 0 1539 1971
Size: 1539 432
BciVI GTATCCnnnnn_n'
Cuts at: 0 175 1971
Size: 175 1796
BclI T'GATC_A
Cuts at: 0 572 1715 1971
Size: 572 1143 256
Fragments arranged by size:
1143 572 256
BfrBI ATG'CAT
Cuts at: 0 1498 1971
Size: 1498 473
Bme1580I G_kGCm'C
Cuts at: 0 504 864 1425 1971
Size: 504 360 561 546
Fragments arranged by size:
561 546 504 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1971
Size: 82 1889
Bpu10I CC'TnA_GC
Cuts at: 0 827 1539 1971
Size: 827 712 432
Fragments arranged by size:
827 712 432
BsaI GGTCTCn'nnnn_
Cuts at: 0 809 1971
Size: 809 1162
BsaAI yAC'GTr
Cuts at: 0 966 1971
Size: 966 1005
BsaBI GATnn'nnATC
Cuts at: 0 1180 1971
Size: 1180 791
BsaHI Gr'CG_yC
Cuts at: 0 1919 1971
Size: 1919 52
BsaWI w'CCGG_w
Cuts at: 0 311 1971
Size: 311 1660
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 438 468 807 837 1217 1247 1971
Size: 438 30 339 30 380 30 724
Fragments arranged by size:
724 438 380 339 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 856 1971
Size: 12 844 1115
Fragments arranged by size:
1115 844 12
BseYI C'CCAG_C
Cuts at: 0 260 1660 1971
Size: 260 1400 311
Fragments arranged by size:
1400 311 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 179 1518 1971
Size: 60 119 1339 453
Fragments arranged by size:
1339 453 119 60
BsiHKAI G_wGCw'C
Cuts at: 0 40 504 531 1425 1971
Size: 40 464 27 894 546
Fragments arranged by size:
894 546 464 40 27
BsmI GAATG_Cn'
Cuts at: 0 1500 1578 1971
Size: 1500 78 393
Fragments arranged by size:
1500 393 78
Bsp1286I G_dGCh'C
Cuts at: 0 40 504 531 864 1425 1834 1971
Size: 40 464 27 333 561 409 137
Fragments arranged by size:
561 464 409 333 137 40 27
BspHI T'CATG_A
Cuts at: 0 1058 1971
Size: 1058 913
BsrDI GCAATG_nn'
Cuts at: 0 1749 1901 1971
Size: 1749 152 70
Fragments arranged by size:
1749 152 70
BsrFI r'CCGG_y
Cuts at: 0 1084 1971
Size: 1084 887
BstAPI GCAn_nnn'nTGC
Cuts at: 0 157 1451 1549 1966 1971
Size: 157 1294 98 417 5
Fragments arranged by size:
1294 417 157 98 5
BstXI CCAn_nnnn'nTGG
Cuts at: 0 970 1971
Size: 970 1001
BstYI r'GATC_y
Cuts at: 0 285 725 1336 1971
Size: 285 440 611 635
Fragments arranged by size:
635 611 440 285
BtgI C'CryG_G
Cuts at: 0 88 1971
Size: 88 1883
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 505 936 1971
Size: 505 431 1035
Fragments arranged by size:
1035 505 431
BtsI GCAGTG_nn'
Cuts at: 0 994 1971
Size: 994 977
DraI TTT'AAA
Cuts at: 0 1487 1971
Size: 1487 484
DraIII CAC_nnn'GTG
Cuts at: 0 337 1429 1971
Size: 337 1092 542
Fragments arranged by size:
1092 542 337
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 1971
Size: 595 1376
EarI CTCTTCn'nnn_
Cuts at: 0 106 115 1971
Size: 106 9 1856
Fragments arranged by size:
1856 106 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6 15 1971
Size: 6 9 1956
Fragments arranged by size:
1956 9 6
EcoICRI GAG'CTC
Cuts at: 0 38 1971
Size: 38 1933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 820 866 1971
Size: 82 738 46 1105
Fragments arranged by size:
1105 738 82 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1627 1971
Size: 1627 344
EcoO109I rG'GnC_Cy
Cuts at: 0 567 861 1971
Size: 567 294 1110
Fragments arranged by size:
1110 567 294
EcoRI G'AATT_C
Cuts at: 0 1843 1971
Size: 1843 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 871 1569 1971
Size: 871 698 402
Fragments arranged by size:
871 698 402
FspI TGC'GCA
Cuts at: 0 931 1971
Size: 931 1040
KpnI G_GTAC'C
Cuts at: 0 1161 1971
Size: 1161 810
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1032 1971
Size: 206 826 939
Fragments arranged by size:
939 826 206
MslI CAynn'nnrTG
Cuts at: 0 121 524 1564 1971
Size: 121 403 1040 407
Fragments arranged by size:
1040 407 403 121
MspA1I CmG'CkG
Cuts at: 0 60 264 1971
Size: 60 204 1707
Fragments arranged by size:
1707 204 60
NcoI C'CATG_G
Cuts at: 0 88 1971
Size: 88 1883
NsiI A_TGCA'T
Cuts at: 0 1500 1971
Size: 1500 471
NspI r_CATG'y
Cuts at: 0 1152 1205 1971
Size: 1152 53 766
Fragments arranged by size:
1152 766 53
PciI A'CATG_T
Cuts at: 0 1148 1971
Size: 1148 823
PflMI CCAn_nnn'nTGG
Cuts at: 0 244 1167 1971
Size: 244 923 804
Fragments arranged by size:
923 804 244
PshAI GACnn'nnGTC
Cuts at: 0 174 1971
Size: 174 1797
PspOMI G'GGCC_C
Cuts at: 0 860 1971
Size: 860 1111
PstI C_TGCA'G
Cuts at: 0 13 1145 1648 1971
Size: 13 1132 503 323
Fragments arranged by size:
1132 503 323 13
SacI G_AGCT'C
Cuts at: 0 40 1971
Size: 40 1931
SapI GCTCTTCn'nnn_
Cuts at: 0 106 1971
Size: 106 1865
ScaI AGT'ACT
Cuts at: 0 716 1971
Size: 716 1255
SfcI C'TryA_G
Cuts at: 0 9 326 887 1141 1644 1971
Size: 9 317 561 254 503 327
Fragments arranged by size:
561 503 327 317 254 9
SpeI A'CTAG_T
Cuts at: 0 171 666 1971
Size: 171 495 1305
Fragments arranged by size:
1305 495 171
StyI C'CwwG_G
Cuts at: 0 88 352 1317 1623 1971
Size: 88 264 965 306 348
Fragments arranged by size:
965 348 306 264 88
SwaI ATTT'AAAT
Cuts at: 0 1487 1971
Size: 1487 484
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 274 1035 1692 1971
Size: 274 761 657 279
Fragments arranged by size:
761 657 279 274
TatI w'GTAC_w
Cuts at: 0 714 771 1049 1145 1971
Size: 714 57 278 96 826
Fragments arranged by size:
826 714 278 96 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 1971
Size: 438 32 1501
Fragments arranged by size:
1501 438 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 684 1971
Size: 684 1287
XmnI GAAnn'nnTTC
Cuts at: 0 1193 1971
Size: 1193 778
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApaI ApaLI ApoI AvrII
BamHI BanI BanII BbsI BbvCI BciVI BclI BfrBI
Bme1580I BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI KpnI MmeI MslI MspA1I
NcoI NsiI NspI PciI PflMI PshAI PspOMI PstI
SacI SapI ScaI SfcI SpeI StyI SwaI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AscI AseI AsiSI AvaI BaeI
BbeI BcgI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI EaeI EagI EcoRV FseI FspAI HaeII Hin4I
HincII HindIII HpaI KasI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI
SrfI SspI StuI Tth111I XbaI XhoI XmaI ZraI