(Linear) (Six-base) MAPSORT of: KIBB9925.seq  Check: 7723  from: 1  to: 1038



KIBB9925.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     463    1038 
   Size:        382      81     575

  Fragments arranged by size: 

                 575     382      81
 
AflIII A'CryG_T

Cuts at:      0     908    1038 
   Size:        908     130
 
AlwNI CAG_nnn'CTG

Cuts at:      0     778    1038 
   Size:        778     260
 
ApoI r'AATT_y

Cuts at:      0     543     677    1038 
   Size:        543     134     361

  Fragments arranged by size: 

                 543     361     134
 
AvaI C'yCGr_G

Cuts at:      0      11     755    1038 
   Size:         11     744     283

  Fragments arranged by size: 

                 744     283      11
 
BanII G_rGCy'C

Cuts at:      0     848    1038 
   Size:        848     190
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     574    1038 
   Size:        574     464
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     724     758    1038 
   Size:        724      34     280

  Fragments arranged by size: 

                 724     280      34
 
BclI T'GATC_A

Cuts at:      0     423    1038 
   Size:        423     615
 
BglII A'GATC_T

Cuts at:      0     178     881    1038 
   Size:        178     703     157

  Fragments arranged by size: 

                 703     178     157
 
Bme1580I G_kGCm'C

Cuts at:      0     390    1038 
   Size:        390     648
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     125    1038 
   Size:        125     913
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862    1022    1038 
   Size:        862     160      16

  Fragments arranged by size: 

                 862     160      16
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     490    1038 
   Size:        490     548
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     995    1025    1038 
   Size:        995      30      13

  Fragments arranged by size: 

                 995      30      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     125     912    1038 
   Size:         94      31     787     126

  Fragments arranged by size: 

                 787     126      94      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     572     776    1038 
   Size:        205     367     204     262

  Fragments arranged by size: 

                 367     262     205     204
 
BsiHKAI G_wGCw'C

Cuts at:      0     848    1033    1038 
   Size:        848     185       5

  Fragments arranged by size: 

                 848     185       5
 
BsmI GAATG_Cn'

Cuts at:      0     745    1038 
   Size:        745     293
 
Bsp1286I G_dGCh'C

Cuts at:      0     390     848    1033    1038 
   Size:        390     458     185       5

  Fragments arranged by size: 

                 458     390     185       5
 
BspHI T'CATG_A

Cuts at:      0     245    1038 
   Size:        245     793
 
BsrDI GCAATG_nn'

Cuts at:      0     863    1038 
   Size:        863     175
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     453    1038 
   Size:        453     585
 
BstYI r'GATC_y

Cuts at:      0     178     881    1038 
   Size:        178     703     157

  Fragments arranged by size: 

                 703     178     157
 
BtsI GCAGTG_nn'

Cuts at:      0      35     302     390    1038 
   Size:         35     267      88     648

  Fragments arranged by size: 

                 648     267      88      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     954    1038 
   Size:        954      84
 
EcoICRI GAG'CTC

Cuts at:      0     846    1038 
   Size:        846     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382     463     862    1022    1038 
   Size:        382      81     399     160      16

  Fragments arranged by size: 

                 399     382     160      81      16
 
EcoRV GAT'ATC

Cuts at:      0     306    1038 
   Size:        306     732
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     995    1027    1038 
   Size:        995      32      11

  Fragments arranged by size: 

                 995      32      11
 
HincII GTy'rAC

Cuts at:      0     827    1038 
   Size:        827     211
 
MslI CAynn'nnrTG

Cuts at:      0     800     913    1038 
   Size:        800     113     125

  Fragments arranged by size: 

                 800     125     113
 
NspI r_CATG'y

Cuts at:      0     912    1038 
   Size:        912     126
 
PciI A'CATG_T

Cuts at:      0     908    1038 
   Size:        908     130
 
PstI C_TGCA'G

Cuts at:      0      30     327     385     458    1038 
   Size:         30     297      58      73     580

  Fragments arranged by size: 

                 580     297      73      58      30
 
SacI G_AGCT'C

Cuts at:      0     848    1038 
   Size:        848     190
 
SfcI C'TryA_G

Cuts at:      0      26     102     323     381     454    1038 
   Size:         26      76     221      58      73     584

  Fragments arranged by size: 

                 584     221      76      73      58      26
 
SmaI CCC'GGG

Cuts at:      0      13    1038 
   Size:         13    1025
 
SmlI C'TyrA_G

Cuts at:      0       5    1038 
   Size:          5    1033
 
SspI AAT'ATT

Cuts at:      0     784     940    1038 
   Size:        784     156      98

  Fragments arranged by size: 

                 784     156      98
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     275    1038 
   Size:        275     763
 
TatI w'GTAC_w

Cuts at:      0      98    1038 
   Size:         98     940
 
XmaI C'CCGG_G

Cuts at:      0      11    1038 
   Size:         11    1027
 
XmnI GAAnn'nnTTC

Cuts at:      0     376    1038 
   Size:        376     662
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI    BanII     BbsI     BcgI
     BclI    BglII Bme1580I     BmrI     BpmI     BsaI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI   BstAPI    BstYI
     BtsI     EarI  EcoICRI  Eco57MI    EcoRV    Hin4I   HincII     MslI
     NspI     PciI     PstI     SacI     SfcI     SmaI     SmlI     SspI
    TaqII     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI
    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI