(Linear) (Six-base) MAPSORT of: KIBB9921.seq  Check: 5347  from: 1  to: 1011



KIBB9921.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:      0     674    1011 
   Size:        674     337
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     842    1011 
   Size:        842     169
 
AleI CACnn'nnGTG

Cuts at:      0     139    1011 
   Size:        139     872
 
ApoI r'AATT_y

Cuts at:      0     182    1011 
   Size:        182     829
 
AvaI C'yCGr_G

Cuts at:      0       9    1011 
   Size:          9    1002
 
BanII G_rGCy'C

Cuts at:      0     253    1011 
   Size:        253     758
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479    1011 
   Size:        479     532
 
Bpu10I CC'TnA_GC

Cuts at:      0     561    1011 
   Size:        561     450
 
BsaHI Gr'CG_yC

Cuts at:      0     671    1011 
   Size:        671     340
 
BsaWI w'CCGG_w

Cuts at:      0     410    1011 
   Size:        410     601
 
BsiHKAI G_wGCw'C

Cuts at:      0     562    1011 
   Size:        562     449
 
Bsp1286I G_dGCh'C

Cuts at:      0     253     562    1011 
   Size:        253     309     449

  Fragments arranged by size: 

                 449     309     253
 
BspHI T'CATG_A

Cuts at:      0     809    1011 
   Size:        809     202
 
BsrDI GCAATG_nn'

Cuts at:      0     851    1011 
   Size:        851     160
 
BsrGI T'GTAC_A

Cuts at:      0     471    1011 
   Size:        471     540
 
BstEII G'GTnAC_C

Cuts at:      0     354    1011 
   Size:        354     657
 
BtsI GCAGTG_nn'

Cuts at:      0      59     350    1011 
   Size:         59     291     661

  Fragments arranged by size: 

                 661     291      59
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     485     520    1011 
   Size:        485      35     491

  Fragments arranged by size: 

                 491     485      35
 
DraI TTT'AAA

Cuts at:      0     596    1011 
   Size:        596     415
 
EaeI y'GGCC_r

Cuts at:      0     833    1011 
   Size:        833     178
 
EarI CTCTTCn'nnn_

Cuts at:      0     914    1011 
   Size:        914      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     842    1011 
   Size:        479     363     169

  Fragments arranged by size: 

                 479     363     169
 
HaeII r_GCGC'y

Cuts at:      0     539    1011 
   Size:        539     472
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       5      37     224     256     946     978    1011 
   Size:          5      32     187      32     690      32      33

  Fragments arranged by size: 

                 690     187      33      32      32      32       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     993    1011 
   Size:        993      18
 
MslI CAynn'nnrTG

Cuts at:      0     139     634    1011 
   Size:        139     495     377

  Fragments arranged by size: 

                 495     377     139
 
MspA1I CmG'CkG

Cuts at:      0     832    1011 
   Size:        832     179
 
PasI CC'CwG_GG

Cuts at:      0     900    1011 
   Size:        900     111
 
PsiI TTA'TAA

Cuts at:      0     160    1011 
   Size:        160     851
 
PstI C_TGCA'G

Cuts at:      0      75     828    1011 
   Size:         75     753     183

  Fragments arranged by size: 

                 753     183      75
 
SexAI A'CCwGG_T

Cuts at:      0     350    1011 
   Size:        350     661
 
SfcI C'TryA_G

Cuts at:      0      71     539     824    1011 
   Size:         71     468     285     187

  Fragments arranged by size: 

                 468     285     187      71
 
StyI C'CwwG_G

Cuts at:      0     254    1011 
   Size:        254     757
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     609    1011 
   Size:        609     402
 
TatI w'GTAC_w

Cuts at:      0     379     471     794    1011 
   Size:        379      92     323     217

  Fragments arranged by size: 

                 379     323     217      92
 
Tth111I GACn'n_nGTC

Cuts at:      0      19    1011 
   Size:         19     992
 

 Enzymes that do cut:

     AccI     AcuI     AleI     ApoI     AvaI    BanII     BpmI   Bpu10I
    BsaHI    BsaWI  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrGI   BstEII
     BtsI    CspCI     DraI     EaeI     EarI  Eco57MI    HaeII    Hin4I
     MmeI     MslI   MspA1I     PasI     PsiI     PstI    SexAI     SfcI
     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI