Restriction map for
pF1KB9919
(Linear) (Six-base) MAPSORT of: KIBB9919.seq Check: 3309 from: 1 to: 912
KIBB9919.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 525 912
Size: 525 387
AccI GT'mk_AC
Cuts at: 0 854 866 912
Size: 854 12 46
Fragments arranged by size:
854 46 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 912
Size: 664 248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 207 375 912
Size: 207 168 537
Fragments arranged by size:
537 207 168
AlwNI CAG_nnn'CTG
Cuts at: 0 214 574 912
Size: 214 360 338
Fragments arranged by size:
360 338 214
ApoI r'AATT_y
Cuts at: 0 267 294 912
Size: 267 27 618
Fragments arranged by size:
618 267 27
BclI T'GATC_A
Cuts at: 0 500 912
Size: 500 412
Bme1580I G_kGCm'C
Cuts at: 0 208 912
Size: 208 704
BmrI ACTGGGnnnn_n'
Cuts at: 0 397 912
Size: 397 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 433 551 869 912
Size: 154 279 118 318 43
Fragments arranged by size:
318 279 154 118 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 912
Size: 757 155
BsaWI w'CCGG_w
Cuts at: 0 660 912
Size: 660 252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 549 579 912
Size: 549 30 333
Fragments arranged by size:
549 333 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 501 912
Size: 277 224 411
Fragments arranged by size:
411 277 224
BseYI C'CCAG_C
Cuts at: 0 201 599 912
Size: 201 398 313
Fragments arranged by size:
398 313 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 912
Size: 124 788
BsiHKAI G_wGCw'C
Cuts at: 0 398 912
Size: 398 514
Bsp1286I G_dGCh'C
Cuts at: 0 208 398 912
Size: 208 190 514
Fragments arranged by size:
514 208 190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 525 912
Size: 525 387
BsrBI CCG'CTC
Cuts at: 0 102 172 912
Size: 102 70 740
Fragments arranged by size:
740 102 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 584 912
Size: 584 328
BtgI C'CryG_G
Cuts at: 0 822 912
Size: 822 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 882 912
Size: 882 30
BtsI GCAGTG_nn'
Cuts at: 0 357 912
Size: 357 555
DraIII CAC_nnn'GTG
Cuts at: 0 584 912
Size: 584 328
EaeI y'GGCC_r
Cuts at: 0 34 440 912
Size: 34 406 472
Fragments arranged by size:
472 406 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 442 912
Size: 442 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 433 551 664 869 912
Size: 154 279 118 113 205 43
Fragments arranged by size:
279 205 154 118 113 43
EcoRI G'AATT_C
Cuts at: 0 267 294 912
Size: 267 27 618
Fragments arranged by size:
618 267 27
HaeII r_GCGC'y
Cuts at: 0 202 912
Size: 202 710
MslI CAynn'nnrTG
Cuts at: 0 379 827 912
Size: 379 448 85
Fragments arranged by size:
448 379 85
MspA1I CmG'CkG
Cuts at: 0 117 571 912
Size: 117 454 341
Fragments arranged by size:
454 341 117
NcoI C'CATG_G
Cuts at: 0 822 912
Size: 822 90
NspI r_CATG'y
Cuts at: 0 720 912
Size: 720 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 574 912
Size: 574 338
PfoI T'CCnGG_A
Cuts at: 0 410 912
Size: 410 502
PvuII CAG'CTG
Cuts at: 0 571 912
Size: 571 341
SexAI A'CCwGG_T
Cuts at: 0 281 912
Size: 281 631
SmlI C'TyrA_G
Cuts at: 0 736 912
Size: 736 176
StuI AGG'CCT
Cuts at: 0 87 846 912
Size: 87 759 66
Fragments arranged by size:
759 87 66
StyI C'CwwG_G
Cuts at: 0 801 822 860 912
Size: 801 21 38 52
Fragments arranged by size:
801 52 38 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 330 912
Size: 142 188 582
Fragments arranged by size:
582 188 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 549 581 912
Size: 549 32 331
Fragments arranged by size:
549 331 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 313 766 912
Size: 313 453 146
Fragments arranged by size:
453 313 146
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApoI BclI Bme1580I
BmrI BpmI BpuEI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BstAPI BtgI BtgZI BtsI
DraIII EaeI EciI Eco57MI EcoRI HaeII MslI MspA1I
NcoI NspI PflMI PfoI PvuII SexAI SmlI StuI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EarI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI