Restriction map for
pF1KB9918
(Linear) (Six-base) MAPSORT of: KIBB9918.seq Check: 5609 from: 1 to: 1737
KIBB9918.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 0 635 1737
Size: 635 1102
AccI GT'mk_AC
Cuts at: 0 4 1737
Size: 4 1733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 614 621 1737
Size: 341 273 7 1116
Fragments arranged by size:
1116 341 273 7
AfeI AGC'GCT
Cuts at: 0 452 1039 1737
Size: 452 587 698
Fragments arranged by size:
698 587 452
AflIII A'CryG_T
Cuts at: 0 670 1172 1737
Size: 670 502 565
Fragments arranged by size:
670 565 502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1031 1737
Size: 1031 706
AlwNI CAG_nnn'CTG
Cuts at: 0 13 1267 1737
Size: 13 1254 470
Fragments arranged by size:
1254 470 13
ApoI r'AATT_y
Cuts at: 0 515 759 1737
Size: 515 244 978
Fragments arranged by size:
978 515 244
BanI G'GyrC_C
Cuts at: 0 588 1737
Size: 588 1149
BanII G_rGCy'C
Cuts at: 0 331 1737
Size: 331 1406
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 627 1737
Size: 29 598 1110
Fragments arranged by size:
1110 598 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 908 1737
Size: 908 829
Bme1580I G_kGCm'C
Cuts at: 0 1459 1737
Size: 1459 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 1292 1737
Size: 1292 445
Bpu10I CC'TnA_GC
Cuts at: 0 767 1737
Size: 767 970
BsaHI Gr'CG_yC
Cuts at: 0 632 1737
Size: 632 1105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 15 18 21 146 1737
Size: 12 3 3 3 125 1591
Fragments arranged by size:
1591 125 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 641 702 815 1737
Size: 641 61 113 922
Fragments arranged by size:
922 641 113 61
BsiEI CG_ry'CG
Cuts at: 0 44 1737
Size: 44 1693
Bsp1286I G_dGCh'C
Cuts at: 0 331 1459 1737
Size: 331 1128 278
Fragments arranged by size:
1128 331 278
BsrDI GCAATG_nn'
Cuts at: 0 361 395 962 1684 1737
Size: 361 34 567 722 53
Fragments arranged by size:
722 567 361 53 34
BsrFI r'CCGG_y
Cuts at: 0 43 1737
Size: 43 1694
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1410 1737
Size: 1410 327
BstYI r'GATC_y
Cuts at: 0 807 1231 1737
Size: 807 424 506
Fragments arranged by size:
807 506 424
Bsu36I CC'TnA_GG
Cuts at: 0 1320 1737
Size: 1320 417
BtgI C'CryG_G
Cuts at: 0 1113 1357 1737
Size: 1113 244 380
Fragments arranged by size:
1113 380 244
BtsI GCAGTG_nn'
Cuts at: 0 1128 1487 1737
Size: 1128 359 250
Fragments arranged by size:
1128 359 250
EaeI y'GGCC_r
Cuts at: 0 41 1737
Size: 41 1696
EagI C'GGCC_G
Cuts at: 0 41 1737
Size: 41 1696
EarI CTCTTCn'nnn_
Cuts at: 0 80 110 123 1737
Size: 80 30 13 1614
Fragments arranged by size:
1614 80 30 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 614 621 1737
Size: 341 273 7 1116
Fragments arranged by size:
1116 341 273 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1621 1737
Size: 1621 116
EcoO109I rG'GnC_Cy
Cuts at: 0 72 465 1737
Size: 72 393 1272
Fragments arranged by size:
1272 393 72
HaeII r_GCGC'y
Cuts at: 0 454 822 1041 1737
Size: 454 368 219 696
Fragments arranged by size:
696 454 368 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 657 689 1737
Size: 657 32 1048
Fragments arranged by size:
1048 657 32
HindIII A'AGCT_T
Cuts at: 0 271 1098 1636 1737
Size: 271 827 538 101
Fragments arranged by size:
827 538 271 101
MluI A'CGCG_T
Cuts at: 0 670 1737
Size: 670 1067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 655 1737
Size: 105 550 1082
Fragments arranged by size:
1082 550 105
MslI CAynn'nnrTG
Cuts at: 0 1000 1408 1737
Size: 1000 408 329
Fragments arranged by size:
1000 408 329
MspA1I CmG'CkG
Cuts at: 0 641 1737
Size: 641 1096
NcoI C'CATG_G
Cuts at: 0 1357 1737
Size: 1357 380
NspI r_CATG'y
Cuts at: 0 441 1176 1737
Size: 441 735 561
Fragments arranged by size:
735 561 441
PciI A'CATG_T
Cuts at: 0 1172 1737
Size: 1172 565
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 1737
Size: 420 1317
PfoI T'CCnGG_A
Cuts at: 0 873 1737
Size: 873 864
PpuMI rG'GwC_Cy
Cuts at: 0 72 465 1737
Size: 72 393 1272
Fragments arranged by size:
1272 393 72
PvuII CAG'CTG
Cuts at: 0 641 1737
Size: 641 1096
SexAI A'CCwGG_T
Cuts at: 0 1266 1737
Size: 1266 471
SfcI C'TryA_G
Cuts at: 0 5 1438 1737
Size: 5 1433 299
Fragments arranged by size:
1433 299 5
SpeI A'CTAG_T
Cuts at: 0 1566 1737
Size: 1566 171
SphI G_CATG'C
Cuts at: 0 441 1737
Size: 441 1296
SspI AAT'ATT
Cuts at: 0 264 1737
Size: 264 1473
StyI C'CwwG_G
Cuts at: 0 414 1357 1737
Size: 414 943 380
Fragments arranged by size:
943 414 380
TatI w'GTAC_w
Cuts at: 0 943 1737
Size: 943 794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1473 1737
Size: 1473 264
ZraI GAC'GTC
Cuts at: 0 633 1737
Size: 633 1104
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AlfI AlwNI ApoI
BanI BanII BbsI BciVI Bme1580I BmrI Bpu10I BsaHI
BseRI BseYI BsiEI Bsp1286I BsrDI BsrFI BstXI BstYI
Bsu36I BtgI BtsI EaeI EagI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII MluI MmeI MslI MspA1I
NcoI NspI PciI PflMI PfoI PpuMI PvuII SexAI
SfcI SpeI SphI SspI StyI TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SrfI StuI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI XmnI