(Linear) (Six-base) MAPSORT of: KIBB9913.seq  Check: 7939  from: 1  to: 855



KIBB9913.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AccI GT'mk_AC

Cuts at:      0     803     855 
   Size:        803      52
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812     855 
   Size:        812      43
 
AleI CACnn'nnGTG

Cuts at:      0     793     855 
   Size:        793      62
 
ApaLI G'TGCA_C

Cuts at:      0     774     855 
   Size:        774      81
 
ApoI r'AATT_y

Cuts at:      0     178     207     855 
   Size:        178      29     648

  Fragments arranged by size: 

                 648     178      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     347     855 
   Size:        347     508
 
Bme1580I G_kGCm'C

Cuts at:      0     778     855 
   Size:        778      77
 
BmgBI CAC'GTC

Cuts at:      0     499     855 
   Size:        499     356
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     760     855 
   Size:        760      95
 
BsaWI w'CCGG_w

Cuts at:      0      12     148     855 
   Size:         12     136     707

  Fragments arranged by size: 

                 707     136      12
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     220     250     855 
   Size:        220      30     605

  Fragments arranged by size: 

                 605     220      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     658     855 
   Size:         70     588     197

  Fragments arranged by size: 

                 588     197      70
 
BsiHKAI G_wGCw'C

Cuts at:      0     778     855 
   Size:        778      77
 
Bsp1286I G_dGCh'C

Cuts at:      0     778     855 
   Size:        778      77
 
BspEI T'CCGG_A

Cuts at:      0      12     855 
   Size:         12     843
 
BsrDI GCAATG_nn'

Cuts at:      0     617     855 
   Size:        617     238
 
BsrFI r'CCGG_y

Cuts at:      0       5      60     855 
   Size:          5      55     795

  Fragments arranged by size: 

                 795      55       5
 
BstEII G'GTnAC_C

Cuts at:      0     357     469     855 
   Size:        357     112     386

  Fragments arranged by size: 

                 386     357     112
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     795     855 
   Size:        795      60
 
BstYI r'GATC_y

Cuts at:      0     256     855 
   Size:        256     599
 
Bsu36I CC'TnA_GG

Cuts at:      0     264     855 
   Size:        264     591
 
BtgI C'CryG_G

Cuts at:      0     474     855 
   Size:        474     381
 
EarI CTCTTCn'nnn_

Cuts at:      0      35     855 
   Size:         35     820
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     812     855 
   Size:        812      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255     483     515     855 
   Size:        223      32     228      32     340

  Fragments arranged by size: 

                 340     228     223      32      32
 
HincII GTy'rAC

Cuts at:      0     502     855 
   Size:        502     353
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     316     418     855 
   Size:        316     102     437

  Fragments arranged by size: 

                 437     316     102
 
MslI CAynn'nnrTG

Cuts at:      0     793     855 
   Size:        793      62
 
NaeI GCC'GGC

Cuts at:      0      62     855 
   Size:         62     793
 
NcoI C'CATG_G

Cuts at:      0     474     855 
   Size:        474     381
 
NgoMIV G'CCGG_C

Cuts at:      0      60     855 
   Size:         60     795
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     480     855 
   Size:        480     375
 
SapI GCTCTTCn'nnn_

Cuts at:      0      35     855 
   Size:         35     820
 
StuI AGG'CCT

Cuts at:      0     445     855 
   Size:        445     410
 
StyI C'CwwG_G

Cuts at:      0     474     855 
   Size:        474     381
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     779     855 
   Size:        779      76
 
TatI w'GTAC_w

Cuts at:      0     351     855 
   Size:        351     504
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     222     855 
   Size:        222     633
 
XmnI GAAnn'nnTTC

Cuts at:      0     821     855 
   Size:        821      34
 

 Enzymes that do cut:

     AccI     AcuI     AleI    ApaLI     ApoI     BbsI Bme1580I    BmgBI
     BmrI    BsaWI    BsaXI    BseRI  BsiHKAI Bsp1286I    BspEI    BsrDI
    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI     EarI  Eco57MI
    Hin4I   HincII     MmeI     MslI     NaeI     NcoI   NgoMIV    PflMI
     SapI     StuI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NarI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI