Restriction map for
pF1KB9900
(Linear) (Six-base) MAPSORT of: KIBB9900.seq Check: 3320 from: 1 to: 1122
KIBB9900.orf
With 182 enzymes: SgfI *
July 3, 2012 16:39 ..
Acc65I G'GTAC_C
Cuts at: 0 950 1085 1122
Size: 950 135 37
Fragments arranged by size:
950 135 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1122
Size: 1014 108
AfeI AGC'GCT
Cuts at: 0 430 1122
Size: 430 692
AflIII A'CryG_T
Cuts at: 0 698 1122
Size: 698 424
AleI CACnn'nnGTG
Cuts at: 0 262 1122
Size: 262 860
AlwNI CAG_nnn'CTG
Cuts at: 0 33 72 464 675 963 1122
Size: 33 39 392 211 288 159
Fragments arranged by size:
392 288 211 159 39 33
ApaI G_GGCC'C
Cuts at: 0 16 865 1122
Size: 16 849 257
Fragments arranged by size:
849 257 16
ApaLI G'TGCA_C
Cuts at: 0 361 1122
Size: 361 761
AvaI C'yCGr_G
Cuts at: 0 110 1122
Size: 110 1012
AvrII C'CTAG_G
Cuts at: 0 133 1122
Size: 133 989
BamHI G'GATC_C
Cuts at: 0 3 127 1122
Size: 3 124 995
Fragments arranged by size:
995 124 3
BanI G'GyrC_C
Cuts at: 0 38 291 470 950 1085 1122
Size: 38 253 179 480 135 37
Fragments arranged by size:
480 253 179 135 38 37
BanII G_rGCy'C
Cuts at: 0 16 174 865 934 1122
Size: 16 158 691 69 188
Fragments arranged by size:
691 188 158 69 16
BbeI G_GCGC'C
Cuts at: 0 42 1122
Size: 42 1080
BciVI GTATCCnnnnn_n'
Cuts at: 0 484 1122
Size: 484 638
BglI GCCn_nnn'nGGC
Cuts at: 0 30 423 835 1122
Size: 30 393 412 287
Fragments arranged by size:
412 393 287 30
Bme1580I G_kGCm'C
Cuts at: 0 16 296 365 851 865 1041 1122
Size: 16 280 69 486 14 176 81
Fragments arranged by size:
486 280 176 81 69 16 14
BmgBI CAC'GTC
Cuts at: 0 245 699 1122
Size: 245 454 423
Fragments arranged by size:
454 423 245
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 383 1122
Size: 378 5 739
Fragments arranged by size:
739 378 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 574 853 1122
Size: 312 262 279 269
Fragments arranged by size:
312 279 269 262
Bpu10I CC'TnA_GC
Cuts at: 0 988 1122
Size: 988 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1122
Size: 673 449
BsaAI yAC'GTr
Cuts at: 0 915 1122
Size: 915 207
BsaHI Gr'CG_yC
Cuts at: 0 39 902 1122
Size: 39 863 220
Fragments arranged by size:
863 220 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 156 186 547 577 694 724 736 766
Size: 156 30 361 30 117 30 12 30
Cuts at: 766 1122
Size: 356
Fragments arranged by size:
361 356 156 117 30 30 30 30
12
BseYI C'CCAG_C
Cuts at: 0 457 858 865 1034 1122
Size: 457 401 7 169 88
Fragments arranged by size:
457 401 169 88 7
BsiEI CG_ry'CG
Cuts at: 0 305 1122
Size: 305 817
BsiHKAI G_wGCw'C
Cuts at: 0 365 1122
Size: 365 757
BsmBI CGTCTCn'nnnn_
Cuts at: 0 107 739 1003 1122
Size: 107 632 264 119
Fragments arranged by size:
632 264 119 107
Bsp1286I G_dGCh'C
Cuts at: 0 16 174 296 365 851 865 934 1041
Size: 16 158 122 69 486 14 69 107
Cuts at: 1041 1122
Size: 81
Fragments arranged by size:
486 158 122 107 81 69 69 16
14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 962 1122
Size: 107 855 160
Fragments arranged by size:
855 160 107
BsrBI CCG'CTC
Cuts at: 0 588 1122
Size: 588 534
BsrFI r'CCGG_y
Cuts at: 0 467 1122
Size: 467 655
BsrGI T'GTAC_A
Cuts at: 0 380 1122
Size: 380 742
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1058 1122
Size: 1058 64
BstXI CCAn_nnnn'nTGG
Cuts at: 0 942 1122
Size: 942 180
BstYI r'GATC_y
Cuts at: 0 3 127 434 1122
Size: 3 124 307 688
Fragments arranged by size:
688 307 124 3
BtgI C'CryG_G
Cuts at: 0 263 559 851 1122
Size: 263 296 292 271
Fragments arranged by size:
296 292 271 263
BtsI GCAGTG_nn'
Cuts at: 0 158 317 1122
Size: 158 159 805
Fragments arranged by size:
805 159 158
EaeI y'GGCC_r
Cuts at: 0 17 50 302 567 599 650 1122
Size: 17 33 252 265 32 51 472
Fragments arranged by size:
472 265 252 51 33 32 17
EagI C'GGCC_G
Cuts at: 0 302 1122
Size: 302 820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 574 853 1014 1122
Size: 312 262 279 161 108
Fragments arranged by size:
312 279 262 161 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 137 727 1122
Size: 137 590 395
Fragments arranged by size:
590 395 137
EcoO109I rG'GnC_Cy
Cuts at: 0 182 862 939 1122
Size: 182 680 77 183
Fragments arranged by size:
680 183 182 77
EcoRV GAT'ATC
Cuts at: 0 375 1122
Size: 375 747
FspI TGC'GCA
Cuts at: 0 1099 1122
Size: 1099 23
HaeII r_GCGC'y
Cuts at: 0 42 432 1122
Size: 42 390 690
Fragments arranged by size:
690 390 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 736 768 1122
Size: 547 32 157 32 354
Fragments arranged by size:
547 354 157 32 32
KasI G'GCGC_C
Cuts at: 0 38 1122
Size: 38 1084
KpnI G_GTAC'C
Cuts at: 0 954 1089 1122
Size: 954 135 33
Fragments arranged by size:
954 135 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 656 1122
Size: 551 105 466
Fragments arranged by size:
551 466 105
MslI CAynn'nnrTG
Cuts at: 0 262 913 917 1122
Size: 262 651 4 205
Fragments arranged by size:
651 262 205 4
MspA1I CmG'CkG
Cuts at: 0 44 69 188 278 1034 1122
Size: 44 25 119 90 756 88
Fragments arranged by size:
756 119 90 88 44 25
NaeI GCC'GGC
Cuts at: 0 469 1122
Size: 469 653
NarI GG'CG_CC
Cuts at: 0 39 1122
Size: 39 1083
NgoMIV G'CCGG_C
Cuts at: 0 467 1122
Size: 467 655
PasI CC'CwG_GG
Cuts at: 0 9 1122
Size: 9 1113
PmlI CAC'GTG
Cuts at: 0 915 1122
Size: 915 207
PpuMI rG'GwC_Cy
Cuts at: 0 182 939 1122
Size: 182 757 183
Fragments arranged by size:
757 183 182
PspOMI G'GGCC_C
Cuts at: 0 12 861 1122
Size: 12 849 261
Fragments arranged by size:
849 261 12
PstI C_TGCA'G
Cuts at: 0 680 959 1073 1122
Size: 680 279 114 49
Fragments arranged by size:
680 279 114 49
PvuII CAG'CTG
Cuts at: 0 69 1034 1122
Size: 69 965 88
Fragments arranged by size:
965 88 69
SanDI GG'GwC_CC
Cuts at: 0 182 1122
Size: 182 940
SbfI CC_TGCA'GG
Cuts at: 0 680 959 1122
Size: 680 279 163
Fragments arranged by size:
680 279 163
SfcI C'TryA_G
Cuts at: 0 676 955 1069 1122
Size: 676 279 114 53
Fragments arranged by size:
676 279 114 53
SfoI GGC'GCC
Cuts at: 0 40 1122
Size: 40 1082
SmlI C'TyrA_G
Cuts at: 0 688 1122
Size: 688 434
StuI AGG'CCT
Cuts at: 0 838 1057 1122
Size: 838 219 65
Fragments arranged by size:
838 219 65
StyI C'CwwG_G
Cuts at: 0 133 728 935 1122
Size: 133 595 207 187
Fragments arranged by size:
595 207 187 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 596 622 1122
Size: 596 26 500
Fragments arranged by size:
596 500 26
TatI w'GTAC_w
Cuts at: 0 380 1122
Size: 380 742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 156 188 1122
Size: 156 32 934
Fragments arranged by size:
934 156 32
Tth111I GACn'n_nGTC
Cuts at: 0 352 750 1122
Size: 352 398 372
Fragments arranged by size:
398 372 352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 32 609 1122
Size: 32 577 513
Fragments arranged by size:
577 513 32
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
AvaI AvrII BamHI BanI BanII BbeI BciVI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI EaeI
EagI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I
KasI KpnI MmeI MslI MspA1I NaeI NarI NgoMIV
PasI PmlI PpuMI PspOMI PstI PvuII SanDI SbfI
SfcI SfoI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI BaeI BbsI BbvCI
BcgI BclI BfrBI BglII BlpI BmtI BplI BsaI
BsaBI BsaWI BseRI BsgI BsiWI BsmI BspEI BspHI
BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoRI FalI FseI FspAI HincII HindIII HpaI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
XmnI ZraI