Restriction map for
pF1KB9887
(Linear) (Six-base) MAPSORT of: KIBB9887.seq Check: 6050 from: 1 to: 2259
KIBB9887.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 33 2259
Size: 33 2226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 2259
Size: 292 1967
AfeI AGC'GCT
Cuts at: 0 279 292 2259
Size: 279 13 1967
Fragments arranged by size:
1967 279 13
AflII C'TTAA_G
Cuts at: 0 771 2259
Size: 771 1488
AhdI GACnn_n'nnGTC
Cuts at: 0 1074 2259
Size: 1074 1185
AlwNI CAG_nnn'CTG
Cuts at: 0 1824 1854 2259
Size: 1824 30 405
Fragments arranged by size:
1824 405 30
ApaI G_GGCC'C
Cuts at: 0 550 2259
Size: 550 1709
ApoI r'AATT_y
Cuts at: 0 1123 2259
Size: 1123 1136
AvaI C'yCGr_G
Cuts at: 0 429 541 565 1000 1545 2259
Size: 429 112 24 435 545 714
Fragments arranged by size:
714 545 435 429 112 24
BanI G'GyrC_C
Cuts at: 0 1359 1575 2163 2259
Size: 1359 216 588 96
Fragments arranged by size:
1359 588 216 96
BanII G_rGCy'C
Cuts at: 0 364 452 550 557 1087 1385 1552 2259
Size: 364 88 98 7 530 298 167 707
Fragments arranged by size:
707 530 364 298 167 98 88 7
BbeI G_GCGC'C
Cuts at: 0 2167 2259
Size: 2167 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 1365 2259
Size: 1365 894
BbvCI CC'TCA_GC
Cuts at: 0 923 1118 1630 2259
Size: 923 195 512 629
Fragments arranged by size:
923 629 512 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1341 1375 2259
Size: 1341 34 884
Fragments arranged by size:
1341 884 34
BclI T'GATC_A
Cuts at: 0 743 2259
Size: 743 1516
Bme1580I G_kGCm'C
Cuts at: 0 472 550 680 717 1258 1364 1602 2259
Size: 472 78 130 37 541 106 238 657
Fragments arranged by size:
657 541 472 238 130 106 78 37
BmgBI CAC'GTC
Cuts at: 0 1638 2259
Size: 1638 621
BmrI ACTGGGnnnn_n'
Cuts at: 0 947 1665 2259
Size: 947 718 594
Fragments arranged by size:
947 718 594
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 383 2259
Size: 383 1876
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1536 1555 2040 2259
Size: 884 652 19 485 219
Fragments arranged by size:
884 652 485 219 19
Bpu10I CC'TnA_GC
Cuts at: 0 377 551 602 923 1118 1630 2140 2259
Size: 377 174 51 321 195 512 510 119
Fragments arranged by size:
512 510 377 321 195 174 119 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1022 2259
Size: 1022 1237
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 440 2259
Size: 414 26 1819
Fragments arranged by size:
1819 414 26
BsaAI yAC'GTr
Cuts at: 0 183 1888 2259
Size: 183 1705 371
Fragments arranged by size:
1705 371 183
BsaHI Gr'CG_yC
Cuts at: 0 1625 1838 1948 2164 2259
Size: 1625 213 110 216 95
Fragments arranged by size:
1625 216 213 110 95
BsaWI w'CCGG_w
Cuts at: 0 533 1620 1833 2259
Size: 533 1087 213 426
Fragments arranged by size:
1087 533 426 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1534 1564 2038 2068 2259
Size: 1534 30 474 30 191
Fragments arranged by size:
1534 474 191 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 907 1018 1117 2259
Size: 286 621 111 99 1142
Fragments arranged by size:
1142 621 286 111 99
BseYI C'CCAG_C
Cuts at: 0 483 1363 2259
Size: 483 880 896
Fragments arranged by size:
896 880 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 1199 1868 2259
Size: 7 1192 669 391
Fragments arranged by size:
1192 669 391 7
BsiEI CG_ry'CG
Cuts at: 0 498 966 1029 1331 2259
Size: 498 468 63 302 928
Fragments arranged by size:
928 498 468 302 63
BsiHKAI G_wGCw'C
Cuts at: 0 233 383 1087 1385 2159 2259
Size: 233 150 704 298 774 100
Fragments arranged by size:
774 704 298 233 150 100
BsmI GAATG_Cn'
Cuts at: 0 1530 2259
Size: 1530 729
BsmBI CGTCTCn'nnnn_
Cuts at: 0 692 1982 2259
Size: 692 1290 277
Fragments arranged by size:
1290 692 277
Bsp1286I G_dGCh'C
Cuts at: 0 233 364 383 452 472 550 557 680
Size: 233 131 19 69 20 78 7 123
Cuts at: 680 717 1087 1258 1364 1385 1552 1602 2159
Size: 37 370 171 106 21 167 50 557
Cuts at: 2159 2259
Size: 100
Fragments arranged by size:
557 370 233 171 167 131 123 106
100 78 69 50 37 21 20 19
7
BspEI T'CCGG_A
Cuts at: 0 533 1620 1833 2259
Size: 533 1087 213 426
Fragments arranged by size:
1087 533 426 213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 1832 2259
Size: 33 1799 427
Fragments arranged by size:
1799 427 33
BsrDI GCAATG_nn'
Cuts at: 0 2157 2259
Size: 2157 102
BsrFI r'CCGG_y
Cuts at: 0 1330 1536 2259
Size: 1330 206 723
Fragments arranged by size:
1330 723 206
BssSI C'ACGA_G
Cuts at: 0 232 1259 2259
Size: 232 1027 1000
Fragments arranged by size:
1027 1000 232
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1824 2259
Size: 1824 435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2202 2259
Size: 2202 57
Bsu36I CC'TnA_GG
Cuts at: 0 269 2259
Size: 269 1990
BtgI C'CryG_G
Cuts at: 0 2033 2168 2259
Size: 2033 135 91
Fragments arranged by size:
2033 135 91
BtsI GCAGTG_nn'
Cuts at: 0 35 1211 1600 2259
Size: 35 1176 389 659
Fragments arranged by size:
1176 659 389 35
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1200 1235 2259
Size: 1200 35 1024
Fragments arranged by size:
1200 1024 35
DraIII CAC_nnn'GTG
Cuts at: 0 30 2259
Size: 30 2229
EaeI y'GGCC_r
Cuts at: 0 53 495 870 963 1328 1940 2171 2216
Size: 53 442 375 93 365 612 231 45
Cuts at: 2216 2259
Size: 43
Fragments arranged by size:
612 442 375 365 231 93 53 45
43
EagI C'GGCC_G
Cuts at: 0 495 963 1328 2259
Size: 495 468 365 931
Fragments arranged by size:
931 495 468 365
EarI CTCTTCn'nnn_
Cuts at: 0 311 576 910 940 1482 2259
Size: 311 265 334 30 542 777
Fragments arranged by size:
777 542 334 311 265 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 93 148 1332 2259
Size: 93 55 1184 927
Fragments arranged by size:
1184 927 93 55
EcoICRI GAG'CTC
Cuts at: 0 1085 1383 2259
Size: 1085 298 876
Fragments arranged by size:
1085 876 298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 884 1536 1555 2040 2259
Size: 292 592 652 19 485 219
Fragments arranged by size:
652 592 485 292 219 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1093 1396 2259
Size: 1093 303 863
Fragments arranged by size:
1093 863 303
EcoO109I rG'GnC_Cy
Cuts at: 0 264 546 547 764 969 1389 1647 2259
Size: 264 282 1 217 205 420 258 612
Fragments arranged by size:
612 420 282 264 258 217 205 1
EcoRI G'AATT_C
Cuts at: 0 1123 2259
Size: 1123 1136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1033 2259
Size: 1033 1226
HaeII r_GCGC'y
Cuts at: 0 165 281 294 1337 2167 2259
Size: 165 116 13 1043 830 92
Fragments arranged by size:
1043 830 165 116 92 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 939 971 1060 1092 1426 1458
Size: 715 32 192 32 89 32 334 32
Cuts at: 1458 1677 1709 2259
Size: 219 32 550
Fragments arranged by size:
715 550 334 219 192 89 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 2163 2259
Size: 2163 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1046 2259
Size: 538 508 1213
Fragments arranged by size:
1213 538 508
MscI TGG'CCA
Cuts at: 0 55 1942 2218 2259
Size: 55 1887 276 41
Fragments arranged by size:
1887 276 55 41
MspA1I CmG'CkG
Cuts at: 0 487 559 869 1698 1851 2035 2072 2170
Size: 487 72 310 829 153 184 37 98
Cuts at: 2170 2211 2259
Size: 41 48
Fragments arranged by size:
829 487 310 184 153 98 72 48
41 37
NaeI GCC'GGC
Cuts at: 0 1332 2259
Size: 1332 927
NarI GG'CG_CC
Cuts at: 0 2164 2259
Size: 2164 95
NgoMIV G'CCGG_C
Cuts at: 0 1330 2259
Size: 1330 929
NruI TCG'CGA
Cuts at: 0 446 2259
Size: 446 1813
PasI CC'CwG_GG
Cuts at: 0 645 717 2259
Size: 645 72 1542
Fragments arranged by size:
1542 645 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 979 2259
Size: 183 796 1280
Fragments arranged by size:
1280 796 183
PfoI T'CCnGG_A
Cuts at: 0 1298 1490 1769 2259
Size: 1298 192 279 490
Fragments arranged by size:
1298 490 279 192
PmlI CAC'GTG
Cuts at: 0 183 1888 2259
Size: 183 1705 371
Fragments arranged by size:
1705 371 183
PpuMI rG'GwC_Cy
Cuts at: 0 264 969 1389 1647 2259
Size: 264 705 420 258 612
Fragments arranged by size:
705 612 420 264 258
PspOMI G'GGCC_C
Cuts at: 0 546 2259
Size: 546 1713
PstI C_TGCA'G
Cuts at: 0 44 1325 1986 2259
Size: 44 1281 661 273
Fragments arranged by size:
1281 661 273 44
PvuII CAG'CTG
Cuts at: 0 487 1698 2259
Size: 487 1211 561
Fragments arranged by size:
1211 561 487
SacI G_AGCT'C
Cuts at: 0 1087 1385 2259
Size: 1087 298 874
Fragments arranged by size:
1087 874 298
SacII CC_GC'GG
Cuts at: 0 2036 2171 2259
Size: 2036 135 88
Fragments arranged by size:
2036 135 88
SanDI GG'GwC_CC
Cuts at: 0 969 2259
Size: 969 1290
SapI GCTCTTCn'nnn_
Cuts at: 0 311 2259
Size: 311 1948
SfcI C'TryA_G
Cuts at: 0 40 1321 1982 2259
Size: 40 1281 661 277
Fragments arranged by size:
1281 661 277 40
SfoI GGC'GCC
Cuts at: 0 2165 2259
Size: 2165 94
SmaI CCC'GGG
Cuts at: 0 567 2259
Size: 567 1692
SmlI C'TyrA_G
Cuts at: 0 771 1037 2259
Size: 771 266 1222
Fragments arranged by size:
1222 771 266
SspI AAT'ATT
Cuts at: 0 88 2259
Size: 88 2171
StuI AGG'CCT
Cuts at: 0 325 921 1402 1557 2259
Size: 325 596 481 155 702
Fragments arranged by size:
702 596 481 325 155
StyI C'CwwG_G
Cuts at: 0 787 887 1250 2219 2259
Size: 787 100 363 969 40
Fragments arranged by size:
969 787 363 100 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 254 2259
Size: 254 2005
TatI w'GTAC_w
Cuts at: 0 1541 2259
Size: 1541 718
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1472 1504 1505 1537 2038 2070 2259
Size: 1472 32 1 32 501 32 189
Fragments arranged by size:
1472 501 189 32 32 32 1
XbaI T'CTAG_A
Cuts at: 0 957 2259
Size: 957 1302
XmaI C'CCGG_G
Cuts at: 0 565 2259
Size: 565 1694
XmnI GAAnn'nnTTC
Cuts at: 0 1670 2259
Size: 1670 589
Enzymes that do cut:
AarI AcuI AfeI AflII AhdI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BssSI BstAPI BstXI Bsu36I BtgI BtsI CspCI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I KasI MmeI MscI MspA1I
NaeI NarI NgoMIV NruI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SfcI SfoI SmaI SmlI SspI StuI StyI TaqII
TatI TstI XbaI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BfrBI BglI BglII BlpI BmtI BsaBI BsiWI
BspHI BsrBI BsrGI BssHII BstBI BstEII BstYI BstZ17I
BtgZI ClaI DraI DrdI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MslI NcoI
NdeI NheI NotI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SwaI Tth111I XcmI XhoI ZraI