Restriction map for
pF1KB9872
(Linear) (Six-base) MAPSORT of: KIBB9872.seq Check: 1485 from: 1 to: 1485
KIBB9872.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1485
Size: 595 890
AflIII A'CryG_T
Cuts at: 0 332 1130 1485
Size: 332 798 355
Fragments arranged by size:
798 355 332
AlwNI CAG_nnn'CTG
Cuts at: 0 942 1485
Size: 942 543
ApaI G_GGCC'C
Cuts at: 0 494 1485
Size: 494 991
AvaI C'yCGr_G
Cuts at: 0 45 433 478 486 568 1485
Size: 45 388 45 8 82 917
Fragments arranged by size:
917 388 82 45 45 8
BanI G'GyrC_C
Cuts at: 0 598 808 1485
Size: 598 210 677
Fragments arranged by size:
677 598 210
BanII G_rGCy'C
Cuts at: 0 487 494 1168 1485
Size: 487 7 674 317
Fragments arranged by size:
674 487 317 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 1084 1114 1485
Size: 1084 30 371
Fragments arranged by size:
1084 371 30
BclI T'GATC_A
Cuts at: 0 119 1485
Size: 119 1366
BlpI GC'TnA_GC
Cuts at: 0 821 1485
Size: 821 664
Bme1580I G_kGCm'C
Cuts at: 0 326 494 601 811 1485
Size: 326 168 107 210 674
Fragments arranged by size:
674 326 210 168 107
BmgBI CAC'GTC
Cuts at: 0 1287 1485
Size: 1287 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 919 1053 1485
Size: 577 342 134 432
Fragments arranged by size:
577 432 342 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1485
Size: 139 1346
BsaBI GATnn'nnATC
Cuts at: 0 124 1485
Size: 124 1361
BsaHI Gr'CG_yC
Cuts at: 0 269 1485
Size: 269 1216
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 337 352 367 1485
Size: 322 15 15 15 1118
Fragments arranged by size:
1118 322 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 385 430 433 1485
Size: 358 27 45 3 1052
Fragments arranged by size:
1052 358 45 27 3
BseYI C'CCAG_C
Cuts at: 0 186 259 730 804 1485
Size: 186 73 471 74 681
Fragments arranged by size:
681 471 186 74 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1485
Size: 1046 439
BsiEI CG_ry'CG
Cuts at: 0 41 249 300 1485
Size: 41 208 51 1185
Fragments arranged by size:
1185 208 51 41
BsiHKAI G_wGCw'C
Cuts at: 0 487 1485
Size: 487 998
BsmBI CGTCTCn'nnnn_
Cuts at: 0 139 519 553 1485
Size: 139 380 34 932
Fragments arranged by size:
932 380 139 34
Bsp1286I G_dGCh'C
Cuts at: 0 326 487 494 601 811 1168 1485
Size: 326 161 7 107 210 357 317
Fragments arranged by size:
357 326 317 210 161 107 7
BspHI T'CATG_A
Cuts at: 0 758 1485
Size: 758 727
BsrFI r'CCGG_y
Cuts at: 0 254 312 1485
Size: 254 58 1173
Fragments arranged by size:
1173 254 58
BssSI C'ACGA_G
Cuts at: 0 97 1485
Size: 97 1388
BstXI CCAn_nnnn'nTGG
Cuts at: 0 861 1485
Size: 861 624
BstYI r'GATC_y
Cuts at: 0 938 1485
Size: 938 547
Bsu36I CC'TnA_GG
Cuts at: 0 226 871 1485
Size: 226 645 614
Fragments arranged by size:
645 614 226
BtgI C'CryG_G
Cuts at: 0 377 623 1485
Size: 377 246 862
Fragments arranged by size:
862 377 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 489 1164 1485
Size: 489 675 321
Fragments arranged by size:
675 489 321
BtsI GCAGTG_nn'
Cuts at: 0 1034 1436 1485
Size: 1034 402 49
Fragments arranged by size:
1034 402 49
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 1485
Size: 9 1476
EaeI y'GGCC_r
Cuts at: 0 38 297 779 863 1485
Size: 38 259 482 84 622
Fragments arranged by size:
622 482 259 84 38
EagI C'GGCC_G
Cuts at: 0 38 297 1485
Size: 38 259 1188
Fragments arranged by size:
1188 259 38
EarI CTCTTCn'nnn_
Cuts at: 0 472 1003 1259 1367 1485
Size: 472 531 256 108 118
Fragments arranged by size:
531 472 256 118 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1060 1485
Size: 1060 425
EcoICRI GAG'CTC
Cuts at: 0 485 1485
Size: 485 1000
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 595 919 1053 1485
Size: 577 18 324 134 432
Fragments arranged by size:
577 432 324 134 18
EcoO109I rG'GnC_Cy
Cuts at: 0 490 930 1485
Size: 490 440 555
Fragments arranged by size:
555 490 440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 759 1485
Size: 759 726
HaeII r_GCGC'y
Cuts at: 0 426 453 1485
Size: 426 27 1032
Fragments arranged by size:
1032 426 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 1142 1174 1485
Size: 322 32 788 32 311
Fragments arranged by size:
788 322 311 32 32
HincII GTy'rAC
Cuts at: 0 318 1485
Size: 318 1167
MfeI C'AATT_G
Cuts at: 0 1145 1196 1428 1485
Size: 1145 51 232 57
Fragments arranged by size:
1145 232 57 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1485
Size: 665 820
MscI TGG'CCA
Cuts at: 0 781 865 1485
Size: 781 84 620
Fragments arranged by size:
781 620 84
NcoI C'CATG_G
Cuts at: 0 377 1485
Size: 377 1108
NotI GC'GGCC_GC
Cuts at: 0 297 1485
Size: 297 1188
NspI r_CATG'y
Cuts at: 0 210 336 1134 1485
Size: 210 126 798 351
Fragments arranged by size:
798 351 210 126
PciI A'CATG_T
Cuts at: 0 332 1130 1485
Size: 332 798 355
Fragments arranged by size:
798 355 332
PflMI CCAn_nnn'nTGG
Cuts at: 0 942 1485
Size: 942 543
PfoI T'CCnGG_A
Cuts at: 0 389 1485
Size: 389 1096
PshAI GACnn'nnGTC
Cuts at: 0 1322 1485
Size: 1322 163
PspOMI G'GGCC_C
Cuts at: 0 490 1485
Size: 490 995
SacI G_AGCT'C
Cuts at: 0 487 1485
Size: 487 998
SapI GCTCTTCn'nnn_
Cuts at: 0 472 1259 1485
Size: 472 787 226
Fragments arranged by size:
787 472 226
ScaI AGT'ACT
Cuts at: 0 238 1485
Size: 238 1247
SfcI C'TryA_G
Cuts at: 0 1115 1485
Size: 1115 370
SmaI CCC'GGG
Cuts at: 0 47 1485
Size: 47 1438
SmlI C'TyrA_G
Cuts at: 0 154 1485
Size: 154 1331
SphI G_CATG'C
Cuts at: 0 210 1485
Size: 210 1275
StyI C'CwwG_G
Cuts at: 0 377 1485
Size: 377 1108
TatI w'GTAC_w
Cuts at: 0 236 1031 1358 1485
Size: 236 795 327 127
Fragments arranged by size:
795 327 236 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 664 696 1276 1308 1485
Size: 664 32 580 32 177
Fragments arranged by size:
664 580 177 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 58 183 1485
Size: 58 125 1302
Fragments arranged by size:
1302 125 58
XmaI C'CCGG_G
Cuts at: 0 45 1485
Size: 45 1440
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BanI BanII BciVI
BclI BlpI Bme1580I BmgBI BpmI BpuEI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I HincII MfeI MmeI MscI
NcoI NotI NspI PciI PflMI PfoI PshAI PspOMI
SacI SapI ScaI SfcI SmaI SmlI SphI StyI
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BfrBI BglI BglII BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaWI BsiWI BsmI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MluI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI