Restriction map for
pF1KB9868
(Linear) (Six-base) MAPSORT of: KIBB9868.seq Check: 6412 from: 1 to: 1350
KIBB9868.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 81 1350
Size: 81 1269
AccI GT'mk_AC
Cuts at: 0 644 1350
Size: 644 706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1350
Size: 448 902
AfeI AGC'GCT
Cuts at: 0 1111 1350
Size: 1111 239
AleI CACnn'nnGTG
Cuts at: 0 682 1120 1350
Size: 682 438 230
Fragments arranged by size:
682 438 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 700 1350
Size: 700 650
ApaI G_GGCC'C
Cuts at: 0 763 781 923 1350
Size: 763 18 142 427
Fragments arranged by size:
763 427 142 18
ApaLI G'TGCA_C
Cuts at: 0 364 1350
Size: 364 986
ApoI r'AATT_y
Cuts at: 0 1225 1350
Size: 1225 125
AvaI C'yCGr_G
Cuts at: 0 68 763 780 931 944 962 1350
Size: 68 695 17 151 13 18 388
Fragments arranged by size:
695 388 151 68 18 17 13
BanI G'GyrC_C
Cuts at: 0 51 64 195 733 823 1350
Size: 51 13 131 538 90 527
Fragments arranged by size:
538 527 131 90 51 13
BanII G_rGCy'C
Cuts at: 0 7 763 781 839 889 923 1350
Size: 7 756 18 58 50 34 427
Fragments arranged by size:
756 427 58 50 34 18 7
BbeI G_GCGC'C
Cuts at: 0 55 68 199 827 1350
Size: 55 13 131 628 523
Fragments arranged by size:
628 523 131 55 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 884 1350
Size: 884 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 138 172 1350
Size: 138 34 1178
Fragments arranged by size:
1178 138 34
BglI GCCn_nnn'nGGC
Cuts at: 0 119 785 849 918 1350
Size: 119 666 64 69 432
Fragments arranged by size:
666 432 119 69 64
BglII A'GATC_T
Cuts at: 0 320 1350
Size: 320 1030
Bme1580I G_kGCm'C
Cuts at: 0 368 736 763 781 923 1350
Size: 368 368 27 18 142 427
Fragments arranged by size:
427 368 368 142 27 18
BmgBI CAC'GTC
Cuts at: 0 339 353 1350
Size: 339 14 997
Fragments arranged by size:
997 339 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 903 1350
Size: 903 447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 899 1350
Size: 898 1 451
Fragments arranged by size:
898 451 1
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1350
Size: 175 1175
BsaAI yAC'GTr
Cuts at: 0 648 1350
Size: 648 702
BsaHI Gr'CG_yC
Cuts at: 0 52 65 196 710 824 1030 1075 1350
Size: 52 13 131 514 114 206 45 275
Fragments arranged by size:
514 275 206 131 114 52 45 13
BsaWI w'CCGG_w
Cuts at: 0 705 1350
Size: 705 645
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 874 904 1133 1163 1350
Size: 874 30 229 30 187
Fragments arranged by size:
874 229 187 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 895 1066 1350
Size: 895 171 284
Fragments arranged by size:
895 284 171
BseYI C'CCAG_C
Cuts at: 0 691 812 1350
Size: 691 121 538
Fragments arranged by size:
691 538 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 1350
Size: 333 1017
BsiEI CG_ry'CG
Cuts at: 0 233 485 551 848 1193 1350
Size: 233 252 66 297 345 157
Fragments arranged by size:
345 297 252 233 157 66
BsiHKAI G_wGCw'C
Cuts at: 0 158 344 368 889 1204 1350
Size: 158 186 24 521 315 146
Fragments arranged by size:
521 315 186 158 146 24
Bsp1286I G_dGCh'C
Cuts at: 0 7 158 344 368 736 763 781 839
Size: 7 151 186 24 368 27 18 58
Cuts at: 839 889 923 1204 1350
Size: 50 34 281 146
Fragments arranged by size:
368 281 186 151 146 58 50 34
27 24 18 7
BspEI T'CCGG_A
Cuts at: 0 705 1350
Size: 705 645
BspHI T'CATG_A
Cuts at: 0 632 1350
Size: 632 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 81 1350
Size: 81 1269
BsrBI CCG'CTC
Cuts at: 0 504 909 1350
Size: 504 405 441
Fragments arranged by size:
504 441 405
BsrFI r'CCGG_y
Cuts at: 0 118 546 843 1032 1350
Size: 118 428 297 189 318
Fragments arranged by size:
428 318 297 189 118
BssHII G'CGCG_C
Cuts at: 0 185 1350
Size: 185 1165
BssSI C'ACGA_G
Cuts at: 0 177 1350
Size: 177 1173
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 756 1350
Size: 384 372 594
Fragments arranged by size:
594 384 372
BstYI r'GATC_y
Cuts at: 0 320 1316 1350
Size: 320 996 34
Fragments arranged by size:
996 320 34
BtgI C'CryG_G
Cuts at: 0 997 1130 1350
Size: 997 133 220
Fragments arranged by size:
997 220 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 150 1350
Size: 150 1200
DraIII CAC_nnn'GTG
Cuts at: 0 470 1350
Size: 470 880
EaeI y'GGCC_r
Cuts at: 0 116 230 245 272 482 548 845 1127
Size: 116 114 15 27 210 66 297 282
Cuts at: 1127 1190 1350
Size: 63 160
Fragments arranged by size:
297 282 210 160 116 114 66 63
27 15
EagI C'GGCC_G
Cuts at: 0 230 482 548 845 1190 1350
Size: 230 252 66 297 345 160
Fragments arranged by size:
345 297 252 230 160 66
EarI CTCTTCn'nnn_
Cuts at: 0 214 349 1225 1350
Size: 214 135 876 125
Fragments arranged by size:
876 214 135 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1288 1350
Size: 1288 62
EcoICRI GAG'CTC
Cuts at: 0 887 1350
Size: 887 463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 898 899 1350
Size: 448 450 1 451
Fragments arranged by size:
451 450 448 1
EcoO109I rG'GnC_Cy
Cuts at: 0 760 919 920 1350
Size: 760 159 1 430
Fragments arranged by size:
760 430 159 1
FseI GG_CCGG'CC
Cuts at: 0 122 1350
Size: 122 1228
FspI TGC'GCA
Cuts at: 0 652 1350
Size: 652 698
FspAI rTGC'GCAy
Cuts at: 0 652 1350
Size: 652 698
HaeII r_GCGC'y
Cuts at: 0 55 68 199 334 827 1113 1350
Size: 55 13 131 135 493 286 237
Fragments arranged by size:
493 286 237 135 131 55 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 874 906 1308 1340 1350
Size: 874 32 402 32 10
Fragments arranged by size:
874 402 32 32 10
KasI G'GCGC_C
Cuts at: 0 51 64 195 823 1350
Size: 51 13 131 628 527
Fragments arranged by size:
628 527 131 51 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1350
Size: 920 430
MscI TGG'CCA
Cuts at: 0 247 274 1350
Size: 247 27 1076
Fragments arranged by size:
1076 247 27
MslI CAynn'nnrTG
Cuts at: 0 169 241 466 682 1120 1141 1350
Size: 169 72 225 216 438 21 209
Fragments arranged by size:
438 225 216 209 169 72 21
MspA1I CmG'CkG
Cuts at: 0 804 999 1350
Size: 804 195 351
Fragments arranged by size:
804 351 195
NaeI GCC'GGC
Cuts at: 0 120 548 845 1034 1350
Size: 120 428 297 189 316
Fragments arranged by size:
428 316 297 189 120
NarI GG'CG_CC
Cuts at: 0 52 65 196 824 1350
Size: 52 13 131 628 526
Fragments arranged by size:
628 526 131 52 13
NgoMIV G'CCGG_C
Cuts at: 0 118 546 843 1032 1350
Size: 118 428 297 189 318
Fragments arranged by size:
428 318 297 189 118
PspOMI G'GGCC_C
Cuts at: 0 759 777 919 1350
Size: 759 18 142 431
Fragments arranged by size:
759 431 142 18
PstI C_TGCA'G
Cuts at: 0 14 92 1041 1350
Size: 14 78 949 309
Fragments arranged by size:
949 309 78 14
SacI G_AGCT'C
Cuts at: 0 889 1350
Size: 889 461
SacII CC_GC'GG
Cuts at: 0 1000 1350
Size: 1000 350
SapI GCTCTTCn'nnn_
Cuts at: 0 349 1225 1350
Size: 349 876 125
Fragments arranged by size:
876 349 125
SbfI CC_TGCA'GG
Cuts at: 0 92 1350
Size: 92 1258
SfcI C'TryA_G
Cuts at: 0 10 88 1037 1350
Size: 10 78 949 313
Fragments arranged by size:
949 313 78 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 918 1350
Size: 918 432
SfoI GGC'GCC
Cuts at: 0 53 66 197 825 1350
Size: 53 13 131 628 525
Fragments arranged by size:
628 525 131 53 13
SmaI CCC'GGG
Cuts at: 0 70 782 946 1350
Size: 70 712 164 404
Fragments arranged by size:
712 404 164 70
SmlI C'TyrA_G
Cuts at: 0 190 1350
Size: 190 1160
SrfI GCCC'GGGC
Cuts at: 0 70 1350
Size: 70 1280
StyI C'CwwG_G
Cuts at: 0 1070 1350
Size: 1070 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 705 826 1350
Size: 61 644 121 524
Fragments arranged by size:
644 524 121 61
TatI w'GTAC_w
Cuts at: 0 419 1350
Size: 419 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1133 1165 1350
Size: 1133 32 185
Fragments arranged by size:
1133 185 32
Tth111I GACn'n_nGTC
Cuts at: 0 712 1350
Size: 712 638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 1350
Size: 282 1068
XmaI C'CCGG_G
Cuts at: 0 68 780 944 1350
Size: 68 712 164 406
Fragments arranged by size:
712 406 164 68
XmnI GAAnn'nnTTC
Cuts at: 0 1111 1350
Size: 1111 239
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AlfI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BcgI BglI
BglII Bme1580I BmgBI BplI BpmI BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstXI
BstYI BtgI BtgZI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FseI FspI FspAI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PspOMI PstI SacI SacII SapI SbfI SfcI SfiI
SfoI SmaI SmlI SrfI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AloI
AlwNI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BlpI BmrI BmtI Bpu10I BsaI
BsaBI BsiWI BsmI BsmBI BsrDI BsrGI BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EcoNI EcoRI EcoRV FalI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SalI SanDI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI XbaI XhoI ZraI