Restriction map for
pF1KB9859
(Linear) (Six-base) MAPSORT of: KIBB9859.seq Check: 4158 from: 1 to: 1200
KIBB9859.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 472 1200
Size: 135 337 728
Fragments arranged by size:
728 337 135
AfeI AGC'GCT
Cuts at: 0 160 449 1200
Size: 160 289 751
Fragments arranged by size:
751 289 160
AflIII A'CryG_T
Cuts at: 0 743 1200
Size: 743 457
AleI CACnn'nnGTG
Cuts at: 0 757 1200
Size: 757 443
AlwNI CAG_nnn'CTG
Cuts at: 0 900 1200
Size: 900 300
ApaI G_GGCC'C
Cuts at: 0 138 1200
Size: 138 1062
AvaI C'yCGr_G
Cuts at: 0 92 610 664 1200
Size: 92 518 54 536
Fragments arranged by size:
536 518 92 54
BanI G'GyrC_C
Cuts at: 0 59 791 1166 1200
Size: 59 732 375 34
Fragments arranged by size:
732 375 59 34
BanII G_rGCy'C
Cuts at: 0 138 199 1200
Size: 138 61 1001
Fragments arranged by size:
1001 138 61
BbeI G_GCGC'C
Cuts at: 0 63 1200
Size: 63 1137
BbsI GAAGACnn'nnnn_
Cuts at: 0 715 1200
Size: 715 485
BbvCI CC'TCA_GC
Cuts at: 0 965 971 1007 1025 1200
Size: 965 6 36 18 175
Fragments arranged by size:
965 175 36 18 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 330 364 762 796 1200
Size: 330 34 398 34 404
Fragments arranged by size:
404 398 330 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1058 1200
Size: 1058 142
BglI GCCn_nnn'nGGC
Cuts at: 0 326 370 865 1164 1200
Size: 326 44 495 299 36
Fragments arranged by size:
495 326 299 44 36
Bme1580I G_kGCm'C
Cuts at: 0 138 149 796 1169 1200
Size: 138 11 647 373 31
Fragments arranged by size:
647 373 138 31 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 635 1200
Size: 635 565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 487 931 1200
Size: 448 39 444 269
Fragments arranged by size:
448 444 269 39
Bpu10I CC'TnA_GC
Cuts at: 0 965 971 1007 1025 1200
Size: 965 6 36 18 175
Fragments arranged by size:
965 175 36 18 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1200
Size: 667 533
BsaHI Gr'CG_yC
Cuts at: 0 60 1200
Size: 60 1140
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 1200
Size: 277 923
BseYI C'CCAG_C
Cuts at: 0 36 81 323 415 959 983 1001 1031
Size: 36 45 242 92 544 24 18 30
Cuts at: 1031 1200
Size: 169
Fragments arranged by size:
544 242 169 92 45 36 30 24
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 859 1200
Size: 676 183 341
Fragments arranged by size:
676 341 183
BsiEI CG_ry'CG
Cuts at: 0 312 626 1200
Size: 312 314 574
Fragments arranged by size:
574 314 312
BsiHKAI G_wGCw'C
Cuts at: 0 199 1200
Size: 199 1001
Bsp1286I G_dGCh'C
Cuts at: 0 138 149 199 796 1169 1200
Size: 138 11 50 597 373 31
Fragments arranged by size:
597 373 138 50 31 11
BsrBI CCG'CTC
Cuts at: 0 264 1200
Size: 264 936
BsrFI r'CCGG_y
Cuts at: 0 12 621 672 1169 1200
Size: 12 609 51 497 31
Fragments arranged by size:
609 497 51 31 12
BssHII G'CGCG_C
Cuts at: 0 293 1200
Size: 293 907
BstAPI GCAn_nnn'nTGC
Cuts at: 0 837 1200
Size: 837 363
BstXI CCAn_nnnn'nTGG
Cuts at: 0 643 1200
Size: 643 557
BtgI C'CryG_G
Cuts at: 0 49 312 713 1200
Size: 49 263 401 487
Fragments arranged by size:
487 401 263 49
DrdI GACnn_nn'nnGTC
Cuts at: 0 228 1200
Size: 228 972
EaeI y'GGCC_r
Cuts at: 0 139 309 623 1200
Size: 139 170 314 577
Fragments arranged by size:
577 314 170 139
EagI C'GGCC_G
Cuts at: 0 309 623 1200
Size: 309 314 577
Fragments arranged by size:
577 314 309
EcoICRI GAG'CTC
Cuts at: 0 197 1200
Size: 197 1003
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 448 472 487 931 1200
Size: 135 313 24 15 444 269
Fragments arranged by size:
444 313 269 135 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1200
Size: 523 677
EcoO109I rG'GnC_Cy
Cuts at: 0 134 203 243 1200
Size: 134 69 40 957
Fragments arranged by size:
957 134 69 40
FseI GG_CCGG'CC
Cuts at: 0 625 1200
Size: 625 575
HaeII r_GCGC'y
Cuts at: 0 27 63 126 162 451 1200
Size: 27 36 63 36 289 749
Fragments arranged by size:
749 289 63 36 36 27
KasI G'GCGC_C
Cuts at: 0 59 1200
Size: 59 1141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1200
Size: 725 475
MslI CAynn'nnrTG
Cuts at: 0 757 1200
Size: 757 443
MspA1I CmG'CkG
Cuts at: 0 51 81 323 558 652 1200
Size: 51 30 242 235 94 548
Fragments arranged by size:
548 242 235 94 51 30
NaeI GCC'GGC
Cuts at: 0 14 623 1200
Size: 14 609 577
Fragments arranged by size:
609 577 14
NarI GG'CG_CC
Cuts at: 0 60 1200
Size: 60 1140
NgoMIV G'CCGG_C
Cuts at: 0 12 621 1200
Size: 12 609 579
Fragments arranged by size:
609 579 12
NspI r_CATG'y
Cuts at: 0 747 1200
Size: 747 453
PciI A'CATG_T
Cuts at: 0 743 1200
Size: 743 457
PfoI T'CCnGG_A
Cuts at: 0 199 1200
Size: 199 1001
PpuMI rG'GwC_Cy
Cuts at: 0 203 243 1200
Size: 203 40 957
Fragments arranged by size:
957 203 40
PspOMI G'GGCC_C
Cuts at: 0 134 1200
Size: 134 1066
PspXI vC'TCGA_Gb
Cuts at: 0 664 1200
Size: 664 536
PstI C_TGCA'G
Cuts at: 0 833 1200
Size: 833 367
PvuII CAG'CTG
Cuts at: 0 558 1200
Size: 558 642
SacI G_AGCT'C
Cuts at: 0 199 1200
Size: 199 1001
SacII CC_GC'GG
Cuts at: 0 52 1200
Size: 52 1148
SanDI GG'GwC_CC
Cuts at: 0 243 1200
Size: 243 957
SexAI A'CCwGG_T
Cuts at: 0 899 1200
Size: 899 301
SfcI C'TryA_G
Cuts at: 0 829 1200
Size: 829 371
SfoI GGC'GCC
Cuts at: 0 61 1200
Size: 61 1139
SmaI CCC'GGG
Cuts at: 0 612 1200
Size: 612 588
SmlI C'TyrA_G
Cuts at: 0 664 682 1200
Size: 664 18 518
Fragments arranged by size:
664 518 18
SrfI GCCC'GGGC
Cuts at: 0 612 1200
Size: 612 588
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 1106 1148 1154 1200
Size: 73 1033 42 6 46
Fragments arranged by size:
1033 73 46 42 6
TatI w'GTAC_w
Cuts at: 0 740 746 842 1200
Size: 740 6 96 358
Fragments arranged by size:
740 358 96 6
XhoI C'TCGA_G
Cuts at: 0 664 1200
Size: 664 536
XmaI C'CCGG_G
Cuts at: 0 610 1200
Size: 610 590
Enzymes that do cut:
AcuI AfeI AflIII AleI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BstAPI BstXI
BtgI DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
FseI HaeII KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PpuMI PspOMI PspXI PstI
PvuII SacI SacII SanDI SexAI SfcI SfoI SmaI
SmlI SrfI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII EarI EciI EcoRI EcoRV FalI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI
TstI Tth111I XbaI XcmI XmnI ZraI