(Linear) (Six-base) MAPSORT of: KIBB9852.seq  Check: 3390  from: 1  to: 1146



KIBB9852.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     780    1146 
   Size:        780     366
 
ApoI r'AATT_y

Cuts at:      0    1026    1146 
   Size:       1026     120
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     256     289    1146 
   Size:        256      33     857

  Fragments arranged by size: 

                 857     256      33
 
BanII G_rGCy'C

Cuts at:      0     766    1146 
   Size:        766     380
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     484    1146 
   Size:        484     662
 
BlpI GC'TnA_GC

Cuts at:      0     760    1146 
   Size:        760     386
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     936    1146 
   Size:        936     210
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30      80    1146 
   Size:         30      50    1066

  Fragments arranged by size: 

                1066      50      30
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     561    1146 
   Size:        322     239     585

  Fragments arranged by size: 

                 585     322     239
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     788    1146 
   Size:        788     358
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      53      83    1146 
   Size:         53      30    1063

  Fragments arranged by size: 

                1063      53      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     804     836    1146 
   Size:        804      32     310

  Fragments arranged by size: 

                 804     310      32
 
BseYI C'CCAG_C

Cuts at:      0     108    1146 
   Size:        108    1038
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     962    1146 
   Size:        962     184
 
Bsp1286I G_dGCh'C

Cuts at:      0     766    1146 
   Size:        766     380
 
BsrBI CCG'CTC

Cuts at:      0     826    1146 
   Size:        826     320
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     355    1146 
   Size:        355     791
 
BstYI r'GATC_y

Cuts at:      0     567    1146 
   Size:        567     579
 
Bsu36I CC'TnA_GG

Cuts at:      0      18    1146 
   Size:         18    1128
 
BtgI C'CryG_G

Cuts at:      0     608    1146 
   Size:        608     538
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     759     794    1146 
   Size:        759      35     352

  Fragments arranged by size: 

                 759     352      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     302     413     484     688    1146 
   Size:        302     111      71     204     458

  Fragments arranged by size: 

                 458     302     204     111      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30      80    1146 
   Size:         30      50    1066

  Fragments arranged by size: 

                1066      50      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      34    1146 
   Size:         34    1112
 
EcoO109I rG'GnC_Cy

Cuts at:      0     234    1146 
   Size:        234     912
 
EcoRI G'AATT_C

Cuts at:      0    1026    1146 
   Size:       1026     120
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     507    1146 
   Size:        507     639
 
HaeII r_GCGC'y

Cuts at:      0      17    1146 
   Size:         17    1129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     766     798    1146 
   Size:        130      32     604      32     348

  Fragments arranged by size: 

                 604     348     130      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     250     634     640    1146 
   Size:        250     384       6     506

  Fragments arranged by size: 

                 506     384     250       6
 
MspA1I CmG'CkG

Cuts at:      0     118    1146 
   Size:        118    1028
 
NspI r_CATG'y

Cuts at:      0     375    1146 
   Size:        375     771
 
PpuMI rG'GwC_Cy

Cuts at:      0     234    1146 
   Size:        234     912
 
PstI C_TGCA'G

Cuts at:      0     242    1146 
   Size:        242     904
 
SapI GCTCTTCn'nnn_

Cuts at:      0     413    1146 
   Size:        413     733
 
ScaI AGT'ACT

Cuts at:      0      83    1146 
   Size:         83    1063
 
SexAI A'CCwGG_T

Cuts at:      0     174    1146 
   Size:        174     972
 
SfcI C'TryA_G

Cuts at:      0     238    1146 
   Size:        238     908
 
SmlI C'TyrA_G

Cuts at:      0     337     540    1146 
   Size:        337     203     606

  Fragments arranged by size: 

                 606     337     203
 
StyI C'CwwG_G

Cuts at:      0     650    1146 
   Size:        650     496
 
TatI w'GTAC_w

Cuts at:      0      81    1146 
   Size:         81    1065
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      53      85    1146 
   Size:         53      32    1061

  Fragments arranged by size: 

                1061      53      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     748    1146 
   Size:        748     398
 
XmnI GAAnn'nnTTC

Cuts at:      0     882    1146 
   Size:        882     264
 

 Enzymes that do cut:

     AhdI     ApoI     BaeI    BanII    BciVI     BlpI     BmrI     BpmI
    BpuEI     BsaI    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BsrBI
    BstXI    BstYI   Bsu36I     BtgI    CspCI     EarI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I     MslI   MspA1I     NspI
    PpuMI     PstI     SapI     ScaI    SexAI     SfcI     SmlI     StyI
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI