Restriction map for
pF1KB9840
(Linear) (Six-base) MAPSORT of: KIBB9840.seq Check: 4705 from: 1 to: 1068
KIBB9840.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
ApaI G_GGCC'C
Cuts at: 0 933 1068
Size: 933 135
AseI AT'TA_AT
Cuts at: 0 905 1068
Size: 905 163
BamHI G'GATC_C
Cuts at: 0 113 1068
Size: 113 955
BanI G'GyrC_C
Cuts at: 0 309 887 895 1068
Size: 309 578 8 173
Fragments arranged by size:
578 309 173 8
BanII G_rGCy'C
Cuts at: 0 933 1068
Size: 933 135
BbeI G_GCGC'C
Cuts at: 0 891 899 1068
Size: 891 8 169
Fragments arranged by size:
891 169 8
BclI T'GATC_A
Cuts at: 0 221 1068
Size: 221 847
BfrBI ATG'CAT
Cuts at: 0 826 1068
Size: 826 242
Bme1580I G_kGCm'C
Cuts at: 0 933 1068
Size: 933 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 315 507 565 946 1011 1068
Size: 315 192 58 381 65 57
Fragments arranged by size:
381 315 192 65 58 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 1068
Size: 525 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 520 661 768 776 1068
Size: 256 264 141 107 8 292
Fragments arranged by size:
292 264 256 141 107 8
BsaBI GATnn'nnATC
Cuts at: 0 142 1068
Size: 142 926
BsaHI Gr'CG_yC
Cuts at: 0 888 896 1068
Size: 888 8 172
Fragments arranged by size:
888 172 8
BsaWI w'CCGG_w
Cuts at: 0 162 763 1068
Size: 162 601 305
Fragments arranged by size:
601 305 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 675 705 1068
Size: 675 30 363
Fragments arranged by size:
675 363 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 954 1068
Size: 136 818 114
Fragments arranged by size:
818 136 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 1068
Size: 621 447
BsmBI CGTCTCn'nnnn_
Cuts at: 0 496 1068
Size: 496 572
Bsp1286I G_dGCh'C
Cuts at: 0 933 1068
Size: 933 135
BspEI T'CCGG_A
Cuts at: 0 162 1068
Size: 162 906
BspHI T'CATG_A
Cuts at: 0 533 1068
Size: 533 535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 378 966 1068
Size: 378 588 102
Fragments arranged by size:
588 378 102
BsrBI CCG'CTC
Cuts at: 0 429 696 1068
Size: 429 267 372
Fragments arranged by size:
429 372 267
BsrFI r'CCGG_y
Cuts at: 0 7 184 312 558 1068
Size: 7 177 128 246 510
Fragments arranged by size:
510 246 177 128 7
BssHII G'CGCG_C
Cuts at: 0 131 1068
Size: 131 937
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1061 1068
Size: 1061 7
BstEII G'GTnAC_C
Cuts at: 0 291 1068
Size: 291 777
BstXI CCAn_nnnn'nTGG
Cuts at: 0 954 1068
Size: 954 114
BstYI r'GATC_y
Cuts at: 0 113 365 911 1068
Size: 113 252 546 157
Fragments arranged by size:
546 252 157 113
Bsu36I CC'TnA_GG
Cuts at: 0 298 1068
Size: 298 770
BtgI C'CryG_G
Cuts at: 0 272 1068
Size: 272 796
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 426 1068
Size: 426 642
DraIII CAC_nnn'GTG
Cuts at: 0 323 470 1068
Size: 323 147 598
Fragments arranged by size:
598 323 147
EaeI y'GGCC_r
Cuts at: 0 275 314 560 588 704 1068
Size: 275 39 246 28 116 364
Fragments arranged by size:
364 275 246 116 39 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 904 1068
Size: 904 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 520 661 768 776 1068
Size: 256 264 141 107 8 292
Fragments arranged by size:
292 264 256 141 107 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 969 1068
Size: 969 99
EcoO109I rG'GnC_Cy
Cuts at: 0 284 929 1068
Size: 284 645 139
Fragments arranged by size:
645 284 139
EcoRV GAT'ATC
Cuts at: 0 246 1068
Size: 246 822
HaeII r_GCGC'y
Cuts at: 0 891 899 1068
Size: 891 8 169
Fragments arranged by size:
891 169 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 649 681 772 804 1068
Size: 649 32 91 32 264
Fragments arranged by size:
649 264 91 32 32
KasI G'GCGC_C
Cuts at: 0 887 895 1068
Size: 887 8 173
Fragments arranged by size:
887 173 8
MscI TGG'CCA
Cuts at: 0 706 1068
Size: 706 362
MspA1I CmG'CkG
Cuts at: 0 274 1068
Size: 274 794
NaeI GCC'GGC
Cuts at: 0 314 560 1068
Size: 314 246 508
Fragments arranged by size:
508 314 246
NarI GG'CG_CC
Cuts at: 0 888 896 1068
Size: 888 8 172
Fragments arranged by size:
888 172 8
NgoMIV G'CCGG_C
Cuts at: 0 312 558 1068
Size: 312 246 510
Fragments arranged by size:
510 312 246
NsiI A_TGCA'T
Cuts at: 0 828 1068
Size: 828 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 1068
Size: 639 429
PshAI GACnn'nnGTC
Cuts at: 0 732 1068
Size: 732 336
PspOMI G'GGCC_C
Cuts at: 0 929 1068
Size: 929 139
SacII CC_GC'GG
Cuts at: 0 275 1068
Size: 275 793
SexAI A'CCwGG_T
Cuts at: 0 716 1068
Size: 716 352
SfoI GGC'GCC
Cuts at: 0 889 897 1068
Size: 889 8 171
Fragments arranged by size:
889 171 8
StyI C'CwwG_G
Cuts at: 0 891 1068
Size: 891 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 646 856 1068
Size: 334 312 210 212
Fragments arranged by size:
334 312 212 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 675 707 1068
Size: 17 32 626 32 361
Fragments arranged by size:
626 361 32 32 17
XbaI T'CTAG_A
Cuts at: 0 654 1068
Size: 654 414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 636 1008 1068
Size: 636 372 60
Fragments arranged by size:
636 372 60
Enzymes that do cut:
ApaI AseI BamHI BanI BanII BbeI BclI BfrBI
Bme1580I BmrI BplI BpmI BsaBI BsaHI BsaWI BsaXI
BseRI BsgI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI DraIII EaeI EciI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I KasI MscI MspA1I NaeI NarI NgoMIV
NsiI PflMI PshAI PspOMI SacII SexAI SfoI StyI
TaqII TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AscI AsiSI AvaI AvrII BaeI BbsI BbvCI
BcgI BciVI BglI BglII BlpI BmgBI BmtI Bpu10I
BpuEI BsaI BsaAI BseYI BsiEI BsiHKAI BsiWI BsmI
BsrDI BsrGI BssSI BstBI BstZ17I BtsI ClaI CspCI
DraI DrdI EagI EarI EcoICRI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MslI NcoI NdeI NheI NotI NruI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XhoI XmaI XmnI ZraI