Restriction map for
pF1KB9838
(Linear) (Six-base) MAPSORT of: KIBB9838.seq Check: 194 from: 1 to: 1056
KIBB9838.orf
With 182 enzymes: SgfI *
August 18, 2011 15:44 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 904 1056
Size: 720 184 152
Fragments arranged by size:
720 184 152
AfeI AGC'GCT
Cuts at: 0 118 673 1056
Size: 118 555 383
Fragments arranged by size:
555 383 118
AleI CACnn'nnGTG
Cuts at: 0 638 1056
Size: 638 418
AlwNI CAG_nnn'CTG
Cuts at: 0 203 1056
Size: 203 853
ApaI G_GGCC'C
Cuts at: 0 301 1056
Size: 301 755
AvaI C'yCGr_G
Cuts at: 0 106 216 280 321 382 403 504 964
Size: 106 110 64 41 61 21 101 460
Cuts at: 964 1056
Size: 92
Fragments arranged by size:
460 110 106 101 92 64 61 41
21
BanI G'GyrC_C
Cuts at: 0 22 220 274 304 316 541 899 1056
Size: 22 198 54 30 12 225 358 157
Fragments arranged by size:
358 225 198 157 54 30 22 12
BanII G_rGCy'C
Cuts at: 0 301 1056
Size: 301 755
BbeI G_GCGC'C
Cuts at: 0 26 278 308 545 903 1056
Size: 26 252 30 237 358 153
Fragments arranged by size:
358 252 237 153 30 26
BglI GCCn_nnn'nGGC
Cuts at: 0 443 490 959 1056
Size: 443 47 469 97
Fragments arranged by size:
469 443 97 47
BlpI GC'TnA_GC
Cuts at: 0 163 1056
Size: 163 893
Bme1580I G_kGCm'C
Cuts at: 0 188 223 301 319 1039 1056
Size: 188 35 78 18 720 17
Fragments arranged by size:
720 188 78 35 18 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 693 1056
Size: 693 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 685 847 862 910 1056
Size: 358 327 162 15 48 146
Fragments arranged by size:
358 327 162 146 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 766 1056
Size: 745 21 290
Fragments arranged by size:
745 290 21
BsaAI yAC'GTr
Cuts at: 0 657 1056
Size: 657 399
BsaHI Gr'CG_yC
Cuts at: 0 23 157 275 305 400 542 900 1056
Size: 23 134 118 30 95 142 358 156
Fragments arranged by size:
358 156 142 134 118 95 30 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 1056
Size: 331 30 695
Fragments arranged by size:
695 331 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 168 180 682 760 1056
Size: 168 12 502 78 296
Fragments arranged by size:
502 296 168 78 12
BseYI C'CCAG_C
Cuts at: 0 34 196 211 1056
Size: 34 162 15 845
Fragments arranged by size:
845 162 34 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1056
Size: 844 212
BsiEI CG_ry'CG
Cuts at: 0 476 1043 1056
Size: 476 567 13
Fragments arranged by size:
567 476 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 266 1056
Size: 266 790
Bsp1286I G_dGCh'C
Cuts at: 0 188 223 301 319 1039 1056
Size: 188 35 78 18 720 17
Fragments arranged by size:
720 188 78 35 18 17
BsrBI CCG'CTC
Cuts at: 0 162 293 359 529 915 1056
Size: 162 131 66 170 386 141
Fragments arranged by size:
386 170 162 141 131 66
BsrFI r'CCGG_y
Cuts at: 0 223 330 544 719 982 1015 1056
Size: 223 107 214 175 263 33 41
Fragments arranged by size:
263 223 214 175 107 41 33
BsrGI T'GTAC_A
Cuts at: 0 923 1056
Size: 923 133
BssHII G'CGCG_C
Cuts at: 0 206 446 458 560 1056
Size: 206 240 12 102 496
Fragments arranged by size:
496 240 206 102 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 173 1056
Size: 173 883
BstXI CCAn_nnnn'nTGG
Cuts at: 0 619 1056
Size: 619 437
BstYI r'GATC_y
Cuts at: 0 342 1056
Size: 342 714
BtgI C'CryG_G
Cuts at: 0 5 441 453 498 555 618 729 789
Size: 5 436 12 45 57 63 111 60
Cuts at: 789 991 1056
Size: 202 65
Fragments arranged by size:
436 202 111 65 63 60 57 45
12 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 31 1056
Size: 31 1025
DraIII CAC_nnn'GTG
Cuts at: 0 657 1056
Size: 657 399
EaeI y'GGCC_r
Cuts at: 0 2 47 435 473 546 905 1040 1056
Size: 2 45 388 38 73 359 135 16
Fragments arranged by size:
388 359 135 73 45 38 16 2
EagI C'GGCC_G
Cuts at: 0 473 1040 1056
Size: 473 567 16
Fragments arranged by size:
567 473 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 539 1056
Size: 539 517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 685 720 847 862 904 910 1056
Size: 358 327 35 127 15 42 6 146
Fragments arranged by size:
358 327 146 127 42 35 15 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 925 1056
Size: 925 131
EcoO109I rG'GnC_Cy
Cuts at: 0 297 298 918 1056
Size: 297 1 620 138
Fragments arranged by size:
620 297 138 1
HaeII r_GCGC'y
Cuts at: 0 26 120 278 308 337 391 424 545
Size: 26 94 158 30 29 54 33 121
Cuts at: 545 675 903 1056
Size: 130 228 153
Fragments arranged by size:
228 158 153 130 121 94 54 33
30 29 26
HincII GTy'rAC
Cuts at: 0 63 1056
Size: 63 993
KasI G'GCGC_C
Cuts at: 0 22 274 304 541 899 1056
Size: 22 252 30 237 358 157
Fragments arranged by size:
358 252 237 157 30 22
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1056
Size: 704 352
MscI TGG'CCA
Cuts at: 0 49 907 1056
Size: 49 858 149
Fragments arranged by size:
858 149 49
MslI CAynn'nnrTG
Cuts at: 0 617 638 703 1056
Size: 617 21 65 353
Fragments arranged by size:
617 353 65 21
MspA1I CmG'CkG
Cuts at: 0 7 45 434 500 690 731 791 831
Size: 7 38 389 66 190 41 60 40
Cuts at: 831 882 993 1056
Size: 51 111 63
Fragments arranged by size:
389 190 111 66 63 60 51 41
40 38 7
NaeI GCC'GGC
Cuts at: 0 332 546 1056
Size: 332 214 510
Fragments arranged by size:
510 332 214
NarI GG'CG_CC
Cuts at: 0 23 275 305 542 900 1056
Size: 23 252 30 237 358 156
Fragments arranged by size:
358 252 237 156 30 23
NcoI C'CATG_G
Cuts at: 0 618 1056
Size: 618 438
NgoMIV G'CCGG_C
Cuts at: 0 330 544 1056
Size: 330 214 512
Fragments arranged by size:
512 330 214
NotI GC'GGCC_GC
Cuts at: 0 473 1056
Size: 473 583
PmlI CAC'GTG
Cuts at: 0 657 1056
Size: 657 399
PpuMI rG'GwC_Cy
Cuts at: 0 918 1056
Size: 918 138
PspOMI G'GGCC_C
Cuts at: 0 297 1056
Size: 297 759
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 778 810 1056
Size: 778 32 246
Fragments arranged by size:
778 246 32
PvuII CAG'CTG
Cuts at: 0 690 882 1056
Size: 690 192 174
Fragments arranged by size:
690 192 174
SacII CC_GC'GG
Cuts at: 0 8 501 732 792 994 1056
Size: 8 493 231 60 202 62
Fragments arranged by size:
493 231 202 62 60 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 959 1056
Size: 959 97
SfoI GGC'GCC
Cuts at: 0 24 276 306 543 901 1056
Size: 24 252 30 237 358 155
Fragments arranged by size:
358 252 237 155 30 24
SgrAI Cr'CCGG_yG
Cuts at: 0 223 1056
Size: 223 833
SmaI CCC'GGG
Cuts at: 0 218 282 506 966 1056
Size: 218 64 224 460 90
Fragments arranged by size:
460 224 218 90 64
SmlI C'TyrA_G
Cuts at: 0 760 781 1056
Size: 760 21 275
Fragments arranged by size:
760 275 21
SrfI GCCC'GGGC
Cuts at: 0 218 506 1056
Size: 218 288 550
Fragments arranged by size:
550 288 218
StyI C'CwwG_G
Cuts at: 0 618 1031 1056
Size: 618 413 25
Fragments arranged by size:
618 413 25
TatI w'GTAC_w
Cuts at: 0 347 923 1056
Size: 347 576 133
Fragments arranged by size:
576 347 133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 616 1056
Size: 42 574 440
Fragments arranged by size:
574 440 42
XmaI C'CCGG_G
Cuts at: 0 216 280 504 964 1056
Size: 216 64 224 460 92
Fragments arranged by size:
460 224 216 92 64
Enzymes that do cut:
AcuI AfeI AleI AlwNI ApaI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BplI BpmI BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I
HaeII HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI PmlI PpuMI PspOMI PsrI
PvuII SacII SfiI SfoI SgrAI SmaI SmlI SrfI
StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI Bpu10I BsaI BsaBI
BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrDI
BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EarI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SnaBI SpeI SphI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmnI ZraI