Restriction map for
pF1KB9835
(Linear) (Six-base) MAPSORT of: KIBB9835.seq Check: 8661 from: 1 to: 1050
KIBB9835.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 72 1050
Size: 72 978
AccI GT'mk_AC
Cuts at: 0 227 1050
Size: 227 823
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1050
Size: 367 683
AleI CACnn'nnGTG
Cuts at: 0 24 1050
Size: 24 1026
AlwNI CAG_nnn'CTG
Cuts at: 0 86 1050
Size: 86 964
ApaI G_GGCC'C
Cuts at: 0 858 1050
Size: 858 192
ApoI r'AATT_y
Cuts at: 0 45 930 1050
Size: 45 885 120
Fragments arranged by size:
885 120 45
BamHI G'GATC_C
Cuts at: 0 608 1050
Size: 608 442
BanII G_rGCy'C
Cuts at: 0 858 1050
Size: 858 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 828 862 1050
Size: 828 34 188
Fragments arranged by size:
828 188 34
BclI T'GATC_A
Cuts at: 0 125 1050
Size: 125 925
BfrBI ATG'CAT
Cuts at: 0 966 1050
Size: 966 84
BlpI GC'TnA_GC
Cuts at: 0 154 1050
Size: 154 896
Bme1580I G_kGCm'C
Cuts at: 0 858 1050
Size: 858 192
BmgBI CAC'GTC
Cuts at: 0 997 1050
Size: 997 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1050
Size: 934 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 342 1050
Size: 342 708
BseYI C'CCAG_C
Cuts at: 0 162 479 1050
Size: 162 317 571
Fragments arranged by size:
571 317 162
BsiEI CG_ry'CG
Cuts at: 0 210 852 1050
Size: 210 642 198
Fragments arranged by size:
642 210 198
Bsp1286I G_dGCh'C
Cuts at: 0 858 1050
Size: 858 192
BspHI T'CATG_A
Cuts at: 0 800 1050
Size: 800 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 72 239 1050
Size: 72 167 811
Fragments arranged by size:
811 167 72
BsrBI CCG'CTC
Cuts at: 0 212 1050
Size: 212 838
BstXI CCAn_nnnn'nTGG
Cuts at: 0 26 1050
Size: 26 1024
BstYI r'GATC_y
Cuts at: 0 15 608 953 1050
Size: 15 593 345 97
Fragments arranged by size:
593 345 97 15
BtgI C'CryG_G
Cuts at: 0 939 1050
Size: 939 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 114 1050
Size: 114 936
EaeI y'GGCC_r
Cuts at: 0 207 248 410 849 924 1050
Size: 207 41 162 439 75 126
Fragments arranged by size:
439 207 162 126 75 41
EagI C'GGCC_G
Cuts at: 0 207 849 1050
Size: 207 642 201
Fragments arranged by size:
642 207 201
EarI CTCTTCn'nnn_
Cuts at: 0 1044 1050
Size: 1044 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 511 609 1050
Size: 511 98 441
Fragments arranged by size:
511 441 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1050
Size: 367 683
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 1050
Size: 328 722
EcoO109I rG'GnC_Cy
Cuts at: 0 567 806 855 1050
Size: 567 239 49 195
Fragments arranged by size:
567 239 195 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 468 1005 1050
Size: 468 537 45
Fragments arranged by size:
537 468 45
HaeII r_GCGC'y
Cuts at: 0 837 1050
Size: 837 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 600 632 1050
Size: 600 32 418
Fragments arranged by size:
600 418 32
HincII GTy'rAC
Cuts at: 0 357 1050
Size: 357 693
MscI TGG'CCA
Cuts at: 0 412 1050
Size: 412 638
MslI CAynn'nnrTG
Cuts at: 0 24 55 324 394 799 1050
Size: 24 31 269 70 405 251
Fragments arranged by size:
405 269 251 70 31 24
MspA1I CmG'CkG
Cuts at: 0 642 1050
Size: 642 408
NcoI C'CATG_G
Cuts at: 0 939 1050
Size: 939 111
NsiI A_TGCA'T
Cuts at: 0 968 1050
Size: 968 82
NspI r_CATG'y
Cuts at: 0 60 1050
Size: 60 990
PfoI T'CCnGG_A
Cuts at: 0 818 1050
Size: 818 232
PpuMI rG'GwC_Cy
Cuts at: 0 567 806 1050
Size: 567 239 244
Fragments arranged by size:
567 244 239
PspOMI G'GGCC_C
Cuts at: 0 854 1050
Size: 854 196
PstI C_TGCA'G
Cuts at: 0 69 1050
Size: 69 981
SexAI A'CCwGG_T
Cuts at: 0 776 1050
Size: 776 274
SfcI C'TryA_G
Cuts at: 0 65 363 1050
Size: 65 298 687
Fragments arranged by size:
687 298 65
SmlI C'TyrA_G
Cuts at: 0 949 1050
Size: 949 101
SphI G_CATG'C
Cuts at: 0 60 1050
Size: 60 990
SspI AAT'ATT
Cuts at: 0 9 1050
Size: 9 1041
StyI C'CwwG_G
Cuts at: 0 245 293 939 1050
Size: 245 48 646 111
Fragments arranged by size:
646 245 111 48
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 873 1050
Size: 873 177
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI BamHI
BanII BcgI BclI BfrBI BlpI Bme1580I BmgBI BpuEI
BsaI BseYI BsiEI Bsp1286I BspHI BspMI BsrBI BstXI
BstYI BtgI BtgZI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII MscI MslI
MspA1I NcoI NsiI NspI PfoI PpuMI PspOMI PstI
SexAI SfcI SmlI SphI SspI StyI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbsI BbvCI BciVI BglI BglII
BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI