Restriction map for
pF1KB9831
(Linear) (Six-base) MAPSORT of: KIBB9831.seq Check: 2695 from: 1 to: 1026
KIBB9831.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 224 1026
Size: 224 802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 998 1026
Size: 692 306 28
Fragments arranged by size:
692 306 28
AleI CACnn'nnGTG
Cuts at: 0 979 1026
Size: 979 47
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 1026
Size: 19 32 975
Fragments arranged by size:
975 32 19
ApaLI G'TGCA_C
Cuts at: 0 702 1026
Size: 702 324
ApoI r'AATT_y
Cuts at: 0 142 259 796 1026
Size: 142 117 537 230
Fragments arranged by size:
537 230 142 117
AseI AT'TA_AT
Cuts at: 0 812 1026
Size: 812 214
AvaI C'yCGr_G
Cuts at: 0 82 953 1026
Size: 82 871 73
Fragments arranged by size:
871 82 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 585 1026
Size: 585 441
BciVI GTATCCnnnnn_n'
Cuts at: 0 242 960 1026
Size: 242 718 66
Fragments arranged by size:
718 242 66
BclI T'GATC_A
Cuts at: 0 200 1026
Size: 200 826
BglII A'GATC_T
Cuts at: 0 158 1026
Size: 158 868
BlpI GC'TnA_GC
Cuts at: 0 773 1026
Size: 773 253
Bme1580I G_kGCm'C
Cuts at: 0 485 706 740 1026
Size: 485 221 34 286
Fragments arranged by size:
485 286 221 34
Bpu10I CC'TnA_GC
Cuts at: 0 377 498 844 1026
Size: 377 121 346 182
Fragments arranged by size:
377 346 182 121
BsaAI yAC'GTr
Cuts at: 0 105 747 1026
Size: 105 642 279
Fragments arranged by size:
642 279 105
BsaBI GATnn'nnATC
Cuts at: 0 157 821 1026
Size: 157 664 205
Fragments arranged by size:
664 205 157
BsaHI Gr'CG_yC
Cuts at: 0 326 1026
Size: 326 700
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 6 1026
Size: 6 1020
BseYI C'CCAG_C
Cuts at: 0 777 1026
Size: 777 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 772 1026
Size: 673 99 254
Fragments arranged by size:
673 254 99
BsiHKAI G_wGCw'C
Cuts at: 0 83 706 1026
Size: 83 623 320
Fragments arranged by size:
623 320 83
Bsp1286I G_dGCh'C
Cuts at: 0 83 485 706 740 1026
Size: 83 402 221 34 286
Fragments arranged by size:
402 286 221 83 34
BsrDI GCAATG_nn'
Cuts at: 0 89 1026
Size: 89 937
BsrFI r'CCGG_y
Cuts at: 0 648 1026
Size: 648 378
BstAPI GCAn_nnn'nTGC
Cuts at: 0 651 1026
Size: 651 375
BstEII G'GTnAC_C
Cuts at: 0 828 1026
Size: 828 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1006 1026
Size: 1006 20
BstYI r'GATC_y
Cuts at: 0 158 1001 1026
Size: 158 843 25
Fragments arranged by size:
843 158 25
BtgI C'CryG_G
Cuts at: 0 179 962 1026
Size: 179 783 64
Fragments arranged by size:
783 179 64
DraIII CAC_nnn'GTG
Cuts at: 0 747 750 1026
Size: 747 3 276
Fragments arranged by size:
747 276 3
EaeI y'GGCC_r
Cuts at: 0 428 1026
Size: 428 598
EarI CTCTTCn'nnn_
Cuts at: 0 55 199 613 1026
Size: 55 144 414 413
Fragments arranged by size:
414 413 144 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 1026
Size: 199 827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 998 1026
Size: 692 306 28
Fragments arranged by size:
692 306 28
FspI TGC'GCA
Cuts at: 0 934 1026
Size: 934 92
HaeII r_GCGC'y
Cuts at: 0 684 691 1026
Size: 684 7 335
Fragments arranged by size:
684 335 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 1026
Size: 32 32 962
Fragments arranged by size:
962 32 32
MscI TGG'CCA
Cuts at: 0 430 1026
Size: 430 596
MslI CAynn'nnrTG
Cuts at: 0 76 424 979 1026
Size: 76 348 555 47
Fragments arranged by size:
555 348 76 47
MspA1I CmG'CkG
Cuts at: 0 756 1026
Size: 756 270
NcoI C'CATG_G
Cuts at: 0 179 1026
Size: 179 847
NspI r_CATG'y
Cuts at: 0 192 933 1026
Size: 192 741 93
Fragments arranged by size:
741 192 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 747 1026
Size: 747 279
PfoI T'CCnGG_A
Cuts at: 0 461 785 1026
Size: 461 324 241
Fragments arranged by size:
461 324 241
PmlI CAC'GTG
Cuts at: 0 747 1026
Size: 747 279
PsiI TTA'TAA
Cuts at: 0 338 1026
Size: 338 688
PvuII CAG'CTG
Cuts at: 0 756 1026
Size: 756 270
SapI GCTCTTCn'nnn_
Cuts at: 0 199 1026
Size: 199 827
SexAI A'CCwGG_T
Cuts at: 0 617 1026
Size: 617 409
SfcI C'TryA_G
Cuts at: 0 924 1026
Size: 924 102
SmaI CCC'GGG
Cuts at: 0 955 1026
Size: 955 71
SphI G_CATG'C
Cuts at: 0 933 1026
Size: 933 93
StyI C'CwwG_G
Cuts at: 0 179 413 1026
Size: 179 234 613
Fragments arranged by size:
613 234 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 1026
Size: 414 612
XmaI C'CCGG_G
Cuts at: 0 953 1026
Size: 953 73
Enzymes that do cut:
AccI AcuI AleI AloI ApaLI ApoI AseI AvaI
BbsI BciVI BclI BglII BlpI Bme1580I Bpu10I BsaAI
BsaBI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BsrFI BstAPI BstEII BstXI BstYI BtgI DraIII EaeI
EarI EciI Eco57MI FspI HaeII Hin4I MscI MslI
MspA1I NcoI NspI PflMI PfoI PmlI PsiI PvuII
SapI SexAI SfcI SmaI SphI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI AscI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BfrBI BglI
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI