Restriction map for
pF1KB9827
(Linear) (Six-base) MAPSORT of: KIBB9827.seq Check: 6007 from: 1 to: 975
KIBB9827.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 120 975
Size: 120 855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 199 549 658 780 975
Size: 112 87 350 109 122 195
Fragments arranged by size:
350 195 122 112 109 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 640 892 975
Size: 640 252 83
Fragments arranged by size:
640 252 83
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 975
Size: 127 32 816
Fragments arranged by size:
816 127 32
AlwNI CAG_nnn'CTG
Cuts at: 0 279 294 572 636 657 926 975
Size: 279 15 278 64 21 269 49
Fragments arranged by size:
279 278 269 64 49 21 15
ApaLI G'TGCA_C
Cuts at: 0 808 975
Size: 808 167
AscI GG'CGCG_CC
Cuts at: 0 411 975
Size: 411 564
AvaI C'yCGr_G
Cuts at: 0 582 943 975
Size: 582 361 32
Fragments arranged by size:
582 361 32
BanI G'GyrC_C
Cuts at: 0 16 975
Size: 16 959
BglI GCCn_nnn'nGGC
Cuts at: 0 169 397 975
Size: 169 228 578
Fragments arranged by size:
578 228 169
BglII A'GATC_T
Cuts at: 0 347 975
Size: 347 628
BlpI GC'TnA_GC
Cuts at: 0 829 975
Size: 829 146
Bme1580I G_kGCm'C
Cuts at: 0 19 812 975
Size: 19 793 163
Fragments arranged by size:
793 163 19
BmgBI CAC'GTC
Cuts at: 0 441 819 975
Size: 441 378 156
Fragments arranged by size:
441 378 156
BmrI ACTGGGnnnn_n'
Cuts at: 0 582 975
Size: 582 393
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 699 975
Size: 699 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 694 975
Size: 301 393 281
Fragments arranged by size:
393 301 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 106 975
Size: 37 69 869
Fragments arranged by size:
869 69 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 106 975
Size: 106 869
BsaHI Gr'CG_yC
Cuts at: 0 161 836 975
Size: 161 675 139
Fragments arranged by size:
675 161 139
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 124 298 490 493 496 514 562
Size: 40 84 174 192 3 3 18 48
Cuts at: 562 975
Size: 413
Fragments arranged by size:
413 192 174 84 48 40 18 3
3
BseYI C'CCAG_C
Cuts at: 0 926 975
Size: 926 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 975
Size: 646 329
BsiEI CG_ry'CG
Cuts at: 0 401 471 975
Size: 401 70 504
Fragments arranged by size:
504 401 70
BsiHKAI G_wGCw'C
Cuts at: 0 812 975
Size: 812 163
Bsp1286I G_dGCh'C
Cuts at: 0 19 812 975
Size: 19 793 163
Fragments arranged by size:
793 163 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 120 975
Size: 120 855
BsrBI CCG'CTC
Cuts at: 0 255 306 785 792 975
Size: 255 51 479 7 183
Fragments arranged by size:
479 255 183 51 7
BsrFI r'CCGG_y
Cuts at: 0 488 590 688 975
Size: 488 102 98 287
Fragments arranged by size:
488 287 102 98
BssHII G'CGCG_C
Cuts at: 0 411 975
Size: 411 564
BstAPI GCAn_nnn'nTGC
Cuts at: 0 37 294 657 975
Size: 37 257 363 318
Fragments arranged by size:
363 318 257 37
BstEII G'GTnAC_C
Cuts at: 0 201 558 975
Size: 201 357 417
Fragments arranged by size:
417 357 201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 975
Size: 192 783
BstYI r'GATC_y
Cuts at: 0 347 975
Size: 347 628
BtgI C'CryG_G
Cuts at: 0 164 842 975
Size: 164 678 133
Fragments arranged by size:
678 164 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 87 747 975
Size: 87 660 228
Fragments arranged by size:
660 228 87
EaeI y'GGCC_r
Cuts at: 0 263 398 975
Size: 263 135 577
Fragments arranged by size:
577 263 135
EagI C'GGCC_G
Cuts at: 0 398 975
Size: 398 577
EarI CTCTTCn'nnn_
Cuts at: 0 799 975
Size: 799 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 675 975
Size: 675 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 199 301 549 658 694 780 975
Size: 112 87 102 248 109 36 86 195
Fragments arranged by size:
248 195 112 109 102 87 86 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 640 975
Size: 640 335
EcoO109I rG'GnC_Cy
Cuts at: 0 234 720 975
Size: 234 486 255
Fragments arranged by size:
486 255 234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 927 975
Size: 927 48
FspI TGC'GCA
Cuts at: 0 610 661 883 975
Size: 610 51 222 92
Fragments arranged by size:
610 222 92 51
HaeII r_GCGC'y
Cuts at: 0 273 649 975
Size: 273 376 326
Fragments arranged by size:
376 326 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 975
Size: 166 32 777
Fragments arranged by size:
777 166 32
MscI TGG'CCA
Cuts at: 0 265 975
Size: 265 710
MspA1I CmG'CkG
Cuts at: 0 72 261 291 312 327 887 926 975
Size: 72 189 30 21 15 560 39 49
Fragments arranged by size:
560 189 72 49 39 30 21 15
NaeI GCC'GGC
Cuts at: 0 690 975
Size: 690 285
NcoI C'CATG_G
Cuts at: 0 842 975
Size: 842 133
NgoMIV G'CCGG_C
Cuts at: 0 688 975
Size: 688 287
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 926 975
Size: 279 647 49
Fragments arranged by size:
647 279 49
PfoI T'CCnGG_A
Cuts at: 0 671 975
Size: 671 304
PpuMI rG'GwC_Cy
Cuts at: 0 720 975
Size: 720 255
PstI C_TGCA'G
Cuts at: 0 299 653 975
Size: 299 354 322
Fragments arranged by size:
354 322 299
PvuII CAG'CTG
Cuts at: 0 261 887 975
Size: 261 626 88
Fragments arranged by size:
626 261 88
SapI GCTCTTCn'nnn_
Cuts at: 0 799 975
Size: 799 176
SfcI C'TryA_G
Cuts at: 0 295 649 975
Size: 295 354 326
Fragments arranged by size:
354 326 295
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 397 975
Size: 397 578
SgrAI Cr'CCGG_yG
Cuts at: 0 590 688 975
Size: 590 98 287
Fragments arranged by size:
590 287 98
SmlI C'TyrA_G
Cuts at: 0 52 121 975
Size: 52 69 854
Fragments arranged by size:
854 69 52
StuI AGG'CCT
Cuts at: 0 934 975
Size: 934 41
StyI C'CwwG_G
Cuts at: 0 842 975
Size: 842 133
TatI w'GTAC_w
Cuts at: 0 8 962 975
Size: 8 954 13
Fragments arranged by size:
954 13 8
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaLI AscI AvaI
BanI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII
BstXI BstYI BtgI BtgZI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I MscI
MspA1I NaeI NcoI NgoMIV PflMI PfoI PpuMI PstI
PvuII SapI SfcI SfiI SgrAI SmlI StuI StyI
TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApoI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRI EcoRV FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NarI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfoI SgfI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI