(Linear) (Six-base) MAPSORT of: KIBB9793.seq Check: 4000 from: 1 to: 636 KIBB9793.orf With 182 enzymes: SgfI * March 3, 2008 11:12 .. AleI CACnn'nnGTG Cuts at: 0 154 636 Size: 154 482 ApaI G_GGCC'C Cuts at: 0 441 636 Size: 441 195 BanII G_rGCy'C Cuts at: 0 92 441 517 636 Size: 92 349 76 119 Fragments arranged by size: 349 119 92 76 BglI GCCn_nnn'nGGC Cuts at: 0 224 636 Size: 224 412 Bme1580I G_kGCm'C Cuts at: 0 441 636 Size: 441 195 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 469 636 Size: 469 167 BsaI GGTCTCn'nnnn_ Cuts at: 0 43 636 Size: 43 593 BsaAI yAC'GTr Cuts at: 0 120 636 Size: 120 516 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 441 471 636 Size: 441 30 165 Fragments arranged by size: 441 165 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 636 Size: 73 563 BseYI C'CCAG_C Cuts at: 0 583 636 Size: 583 53 BsiHKAI G_wGCw'C Cuts at: 0 517 636 Size: 517 119 BsmI GAATG_Cn' Cuts at: 0 477 636 Size: 477 159 Bsp1286I G_dGCh'C Cuts at: 0 92 441 517 636 Size: 92 349 76 119 Fragments arranged by size: 349 119 92 76 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 371 636 Size: 371 265 BtgI C'CryG_G Cuts at: 0 23 53 170 290 431 557 636 Size: 23 30 117 120 141 126 79 Fragments arranged by size: 141 126 120 117 79 30 23 BtsI GCAGTG_nn' Cuts at: 0 283 594 636 Size: 283 311 42 Fragments arranged by size: 311 283 42 EaeI y'GGCC_r Cuts at: 0 270 636 Size: 270 366 EcoICRI GAG'CTC Cuts at: 0 515 636 Size: 515 121 EcoO109I rG'GnC_Cy Cuts at: 0 5 76 360 437 438 636 Size: 5 71 284 77 1 198 Fragments arranged by size: 284 198 77 71 5 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 551 636 Size: 551 85 MscI TGG'CCA Cuts at: 0 272 636 Size: 272 364 MslI CAynn'nnrTG Cuts at: 0 154 636 Size: 154 482 NcoI C'CATG_G Cuts at: 0 290 431 557 636 Size: 290 141 126 79 Fragments arranged by size: 290 141 126 79 PasI CC'CwG_GG Cuts at: 0 177 636 Size: 177 459 PflMI CCAn_nnn'nTGG Cuts at: 0 558 636 Size: 558 78 PfoI T'CCnGG_A Cuts at: 0 78 636 Size: 78 558 PpuMI rG'GwC_Cy Cuts at: 0 5 76 360 636 Size: 5 71 284 276 Fragments arranged by size: 284 276 71 5 PspOMI G'GGCC_C Cuts at: 0 437 636 Size: 437 199 SacI G_AGCT'C Cuts at: 0 517 636 Size: 517 119 StuI AGG'CCT Cuts at: 0 214 636 Size: 214 422 StyI C'CwwG_G Cuts at: 0 290 367 431 557 636 Size: 290 77 64 126 79 Fragments arranged by size: 290 126 79 77 64 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 441 473 636 Size: 441 32 163 Fragments arranged by size: 441 163 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 156 288 636 Size: 156 132 348 Fragments arranged by size: 348 156 132 Enzymes that do cut: AleI ApaI BanII BglI Bme1580I BplI BsaI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BtgI BtsI EaeI EcoICRI EcoO109I FalI MscI MslI NcoI PasI PflMI PfoI PpuMI PspOMI SacI StuI StyI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI