(Linear) (Six-base) MAPSORT of: KIBB9781.seq  Check: 1506  from: 1  to: 360



KIBB9781.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     132     360 
   Size:        132     228
 
BanII G_rGCy'C

Cuts at:      0     126     360 
   Size:        126     234
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     340     360 
   Size:        340      20
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     174     360 
   Size:        174     186
 
Bme1580I G_kGCm'C

Cuts at:      0     192     360 
   Size:        192     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     360 
   Size:        299      61
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     294     360 
   Size:        109     185      66

  Fragments arranged by size: 

                 185     109      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      14      44     360 
   Size:         14      30     316

  Fragments arranged by size: 

                 316      30      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     108     360 
   Size:        108     252
 
BseYI C'CCAG_C

Cuts at:      0     328     360 
   Size:        328      32
 
BsmI GAATG_Cn'

Cuts at:      0     121     360 
   Size:        121     239
 
Bsp1286I G_dGCh'C

Cuts at:      0     126     192     360 
   Size:        126      66     168

  Fragments arranged by size: 

                 168     126      66
 
BsrDI GCAATG_nn'

Cuts at:      0     239     360 
   Size:        239     121
 
BsrFI r'CCGG_y

Cuts at:      0      98     360 
   Size:         98     262
 
BsrGI T'GTAC_A

Cuts at:      0     212     360 
   Size:        212     148
 
BtsI GCAGTG_nn'

Cuts at:      0     226     360 
   Size:        226     134
 
EarI CTCTTCn'nnn_

Cuts at:      0     149     278     360 
   Size:        149     129      82

  Fragments arranged by size: 

                 149     129      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     299     360 
   Size:        299      61
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     196     360 
   Size:        196     164
 
EcoO109I rG'GnC_Cy

Cuts at:      0     267     360 
   Size:        267      93
 
EcoRV GAT'ATC

Cuts at:      0     142     360 
   Size:        142     218
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     103     360 
   Size:        103     257
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     148     180     360 
   Size:        148      32     180

  Fragments arranged by size: 

                 180     148      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     360 
   Size:         34     326
 
MslI CAynn'nnrTG

Cuts at:      0     232     360 
   Size:        232     128
 
NaeI GCC'GGC

Cuts at:      0     100     360 
   Size:        100     260
 
NgoMIV G'CCGG_C

Cuts at:      0      98     360 
   Size:         98     262
 
SapI GCTCTTCn'nnn_

Cuts at:      0     278     360 
   Size:        278      82
 
SmlI C'TyrA_G

Cuts at:      0      88     309     360 
   Size:         88     221      51

  Fragments arranged by size: 

                 221      88      51
 
StyI C'CwwG_G

Cuts at:      0     240     323     360 
   Size:        240      83      37

  Fragments arranged by size: 

                 240      83      37
 
TatI w'GTAC_w

Cuts at:      0     212     360 
   Size:        212     148
 

 Enzymes that do cut:

     AlfI    BanII     BbsI    BciVI Bme1580I     BpmI    BpuEI    BsaXI
    BseRI    BseYI     BsmI Bsp1286I    BsrDI    BsrFI    BsrGI     BtsI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    Hin4I     MmeI
     MslI     NaeI   NgoMIV     SapI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI