Restriction map for
pF1KB9779
(Linear) (Six-base) MAPSORT of: KIBB9779.seq Check: 3267 from: 1 to: 2661
KIBB9779.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2432 2661
Size: 2432 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 2093 2661
Size: 1337 756 568
Fragments arranged by size:
1337 756 568
AflII C'TTAA_G
Cuts at: 0 61 2661
Size: 61 2600
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2443 2475 2661
Size: 2443 32 186
Fragments arranged by size:
2443 186 32
AlwNI CAG_nnn'CTG
Cuts at: 0 361 2065 2583 2661
Size: 361 1704 518 78
Fragments arranged by size:
1704 518 361 78
ApoI r'AATT_y
Cuts at: 0 658 789 994 1218 1293 1305 1749 2533
Size: 658 131 205 224 75 12 444 784
Cuts at: 2533 2661
Size: 128
Fragments arranged by size:
784 658 444 224 205 131 128 75
12
BamHI G'GATC_C
Cuts at: 0 2304 2661
Size: 2304 357
BanI G'GyrC_C
Cuts at: 0 2231 2661
Size: 2231 430
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 2661
Size: 125 2536
BbvCI CC'TCA_GC
Cuts at: 0 2156 2661
Size: 2156 505
BclI T'GATC_A
Cuts at: 0 2201 2661
Size: 2201 460
BfrBI ATG'CAT
Cuts at: 0 420 2661
Size: 420 2241
BglI GCCn_nnn'nGGC
Cuts at: 0 316 1039 2661
Size: 316 723 1622
Fragments arranged by size:
1622 723 316
BglII A'GATC_T
Cuts at: 0 939 1419 2661
Size: 939 480 1242
Fragments arranged by size:
1242 939 480
Bme1580I G_kGCm'C
Cuts at: 0 1845 2368 2661
Size: 1845 523 293
Fragments arranged by size:
1845 523 293
BmrI ACTGGGnnnn_n'
Cuts at: 0 2640 2661
Size: 2640 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 2661
Size: 177 2484
Bpu10I CC'TnA_GC
Cuts at: 0 2156 2661
Size: 2156 505
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 2661
Size: 1010 1651
BsaWI w'CCGG_w
Cuts at: 0 1815 2661
Size: 1815 846
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 459 489 2661
Size: 459 30 2172
Fragments arranged by size:
2172 459 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1358 2661
Size: 1358 1303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1613 1838 2661
Size: 1613 225 823
Fragments arranged by size:
1613 823 225
BsmI GAATG_Cn'
Cuts at: 0 312 2096 2661
Size: 312 1784 565
Fragments arranged by size:
1784 565 312
Bsp1286I G_dGCh'C
Cuts at: 0 1845 2368 2661
Size: 1845 523 293
Fragments arranged by size:
1845 523 293
BspEI T'CCGG_A
Cuts at: 0 1815 2661
Size: 1815 846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 2432 2661
Size: 305 2127 229
Fragments arranged by size:
2127 305 229
BsrDI GCAATG_nn'
Cuts at: 0 1723 2661
Size: 1723 938
BssSI C'ACGA_G
Cuts at: 0 873 2661
Size: 873 1788
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2574 2661
Size: 2574 87
BstYI r'GATC_y
Cuts at: 0 939 1017 1419 1527 2304 2661
Size: 939 78 402 108 777 357
Fragments arranged by size:
939 777 402 357 108 78
BtsI GCAGTG_nn'
Cuts at: 0 362 2624 2661
Size: 362 2262 37
Fragments arranged by size:
2262 362 37
DraI TTT'AAA
Cuts at: 0 1836 2661
Size: 1836 825
DraIII CAC_nnn'GTG
Cuts at: 0 561 2128 2374 2661
Size: 561 1567 246 287
Fragments arranged by size:
1567 561 287 246
DrdI GACnn_nn'nnGTC
Cuts at: 0 430 2661
Size: 430 2231
EarI CTCTTCn'nnn_
Cuts at: 0 79 1365 2275 2321 2552 2661
Size: 79 1286 910 46 231 109
Fragments arranged by size:
1286 910 231 109 79 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 2093 2661
Size: 1337 756 568
Fragments arranged by size:
1337 756 568
EcoNI CCTnn'n_nnAGG
Cuts at: 0 365 2661
Size: 365 2296
EcoRV GAT'ATC
Cuts at: 0 48 1439 2661
Size: 48 1391 1222
Fragments arranged by size:
1391 1222 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 857 1951 2661
Size: 857 1094 710
Fragments arranged by size:
1094 857 710
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 504 536 712 744 1084 1116 2661
Size: 504 32 176 32 340 32 1545
Fragments arranged by size:
1545 504 340 176 32 32 32
HindIII A'AGCT_T
Cuts at: 0 72 814 2661
Size: 72 742 1847
Fragments arranged by size:
1847 742 72
MfeI C'AATT_G
Cuts at: 0 1192 2661
Size: 1192 1469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2202 2531 2661
Size: 2202 329 130
Fragments arranged by size:
2202 329 130
MslI CAynn'nnrTG
Cuts at: 0 1913 2661
Size: 1913 748
NdeI CA'TA_TG
Cuts at: 0 328 927 2661
Size: 328 599 1734
Fragments arranged by size:
1734 599 328
NsiI A_TGCA'T
Cuts at: 0 422 2661
Size: 422 2239
NspI r_CATG'y
Cuts at: 0 161 405 2577 2661
Size: 161 244 2172 84
Fragments arranged by size:
2172 244 161 84
PfoI T'CCnGG_A
Cuts at: 0 113 345 2661
Size: 113 232 2316
Fragments arranged by size:
2316 232 113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1406 1438 2661
Size: 1406 32 1223
Fragments arranged by size:
1406 1223 32
PsiI TTA'TAA
Cuts at: 0 104 1336 2009 2661
Size: 104 1232 673 652
Fragments arranged by size:
1232 673 652 104
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2374 2406 2661
Size: 2374 32 255
Fragments arranged by size:
2374 255 32
PstI C_TGCA'G
Cuts at: 0 1314 2619 2661
Size: 1314 1305 42
Fragments arranged by size:
1314 1305 42
ScaI AGT'ACT
Cuts at: 0 2650 2661
Size: 2650 11
SfcI C'TryA_G
Cuts at: 0 30 362 1310 1717 2615 2661
Size: 30 332 948 407 898 46
Fragments arranged by size:
948 898 407 332 46 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1039 2661
Size: 1039 1622
SmlI C'TyrA_G
Cuts at: 0 61 1025 2661
Size: 61 964 1636
Fragments arranged by size:
1636 964 61
SspI AAT'ATT
Cuts at: 0 891 2661
Size: 891 1770
StuI AGG'CCT
Cuts at: 0 1042 2661
Size: 1042 1619
StyI C'CwwG_G
Cuts at: 0 411 1043 1088 2661
Size: 411 632 45 1573
Fragments arranged by size:
1573 632 411 45
TatI w'GTAC_w
Cuts at: 0 244 1547 1674 2026 2648 2661
Size: 244 1303 127 352 622 13
Fragments arranged by size:
1303 622 352 244 127 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 341 373 459 491 545 577 2183 2215
Size: 341 32 86 32 54 32 1606 32
Cuts at: 2215 2661
Size: 446
Fragments arranged by size:
1606 446 341 86 54 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1041 2281 2661
Size: 1041 1240 380
Fragments arranged by size:
1240 1041 380
XmnI GAAnn'nnTTC
Cuts at: 0 1305 2433 2630 2661
Size: 1305 1128 197 31
Fragments arranged by size:
1305 1128 197 31
Enzymes that do cut:
AarI AcuI AflII AloI AlwNI ApoI BamHI BanI
BbsI BbvCI BclI BfrBI BglI BglII Bme1580I BmrI
BplI Bpu10I BpuEI BsaWI BsaXI BseRI BsgI BsmI
Bsp1286I BspEI BspMI BsrDI BssSI BstAPI BstYI BtsI
DraI DraIII DrdI EarI Eco57MI EcoNI EcoRV FalI
Hin4I HindIII MfeI MmeI MslI NdeI NsiI NspI
PfoI PpiI PsiI PsrI PstI ScaI SfcI SfiI
SmlI SspI StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BcgI BciVI BlpI BmgBI
BmtI BpmI BsaI BsaAI BsaBI BsaHI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI EaeI EagI EciI EcoICRI EcoO109I EcoRI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MluI
MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpuMI
PshAI PspOMI PspXI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI