Restriction map for
pF1KB9770
(Linear) (Six-base) MAPSORT of: KIBB9770.seq Check: 9200 from: 1 to: 1386
KIBB9770.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 517 1386
Size: 517 869
AccI GT'mk_AC
Cuts at: 0 664 1386
Size: 664 722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 1386
Size: 243 1143
AgeI A'CCGG_T
Cuts at: 0 554 1386
Size: 554 832
AleI CACnn'nnGTG
Cuts at: 0 40 835 1386
Size: 40 795 551
Fragments arranged by size:
795 551 40
ApaI G_GGCC'C
Cuts at: 0 187 220 1386
Size: 187 33 1166
Fragments arranged by size:
1166 187 33
AvaI C'yCGr_G
Cuts at: 0 129 1386
Size: 129 1257
BamHI G'GATC_C
Cuts at: 0 701 854 968 1386
Size: 701 153 114 418
Fragments arranged by size:
701 418 153 114
BanI G'GyrC_C
Cuts at: 0 234 557 1027 1368 1386
Size: 234 323 470 341 18
Fragments arranged by size:
470 341 323 234 18
BanII G_rGCy'C
Cuts at: 0 166 187 220 731 1148 1289 1386
Size: 166 21 33 511 417 141 97
Fragments arranged by size:
511 417 166 141 97 33 21
BbeI G_GCGC'C
Cuts at: 0 1031 1372 1386
Size: 1031 341 14
Fragments arranged by size:
1031 341 14
BbvCI CC'TCA_GC
Cuts at: 0 1174 1386
Size: 1174 212
BglI GCCn_nnn'nGGC
Cuts at: 0 341 1386
Size: 341 1045
BlpI GC'TnA_GC
Cuts at: 0 280 713 1247 1386
Size: 280 433 534 139
Fragments arranged by size:
534 433 280 139
Bme1580I G_kGCm'C
Cuts at: 0 187 220 239 1386
Size: 187 33 19 1147
Fragments arranged by size:
1147 187 33 19
BmgBI CAC'GTC
Cuts at: 0 993 1386
Size: 993 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1384 1386
Size: 727 657 2
Fragments arranged by size:
727 657 2
Bpu10I CC'TnA_GC
Cuts at: 0 160 1174 1386
Size: 160 1014 212
Fragments arranged by size:
1014 212 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1386
Size: 334 1052
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 1386
Size: 733 653
BsaAI yAC'GTr
Cuts at: 0 747 1386
Size: 747 639
BsaHI Gr'CG_yC
Cuts at: 0 344 1028 1369 1386
Size: 344 684 341 17
Fragments arranged by size:
684 344 341 17
BsaWI w'CCGG_w
Cuts at: 0 554 641 998 1386
Size: 554 87 357 388
Fragments arranged by size:
554 388 357 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1045 1075 1120 1150 1386
Size: 1045 30 45 30 236
Fragments arranged by size:
1045 236 45 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 414 634 1386
Size: 39 375 220 752
Fragments arranged by size:
752 375 220 39
BseYI C'CCAG_C
Cuts at: 0 275 772 1098 1386
Size: 275 497 326 288
Fragments arranged by size:
497 326 288 275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 634 973 1386
Size: 82 552 339 413
Fragments arranged by size:
552 413 339 82
BsiEI CG_ry'CG
Cuts at: 0 1160 1386
Size: 1160 226
BsiHKAI G_wGCw'C
Cuts at: 0 166 1386
Size: 166 1220
BsmI GAATG_Cn'
Cuts at: 0 141 1386
Size: 141 1245
Bsp1286I G_dGCh'C
Cuts at: 0 166 187 220 239 731 1148 1289 1386
Size: 166 21 33 19 492 417 141 97
Fragments arranged by size:
492 417 166 141 97 33 21 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 517 1386
Size: 517 869
BsrBI CCG'CTC
Cuts at: 0 24 422 624 947 1386
Size: 24 398 202 323 439
Fragments arranged by size:
439 398 323 202 24
BsrFI r'CCGG_y
Cuts at: 0 554 690 1266 1386
Size: 554 136 576 120
Fragments arranged by size:
576 554 136 120
BsrGI T'GTAC_A
Cuts at: 0 446 1386
Size: 446 940
BssHII G'CGCG_C
Cuts at: 0 1110 1386
Size: 1110 276
BstYI r'GATC_y
Cuts at: 0 701 854 968 1386
Size: 701 153 114 418
Fragments arranged by size:
701 418 153 114
Bsu36I CC'TnA_GG
Cuts at: 0 369 426 1386
Size: 369 57 960
Fragments arranged by size:
960 369 57
BtgI C'CryG_G
Cuts at: 0 80 212 590 722 1386
Size: 80 132 378 132 664
Fragments arranged by size:
664 378 132 132 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 912 936 1386
Size: 912 24 450
Fragments arranged by size:
912 450 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 889 1386
Size: 889 497
EaeI y'GGCC_r
Cuts at: 0 587 719 977 1157 1386
Size: 587 132 258 180 229
Fragments arranged by size:
587 258 229 180 132
EagI C'GGCC_G
Cuts at: 0 1157 1386
Size: 1157 229
EarI CTCTTCn'nnn_
Cuts at: 0 1312 1386
Size: 1312 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1249 1386
Size: 1249 137
EcoICRI GAG'CTC
Cuts at: 0 164 1386
Size: 164 1222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 727 1384 1386
Size: 243 484 657 2
Fragments arranged by size:
657 484 243 2
EcoO109I rG'GnC_Cy
Cuts at: 0 112 217 354 495 1386
Size: 112 105 137 141 891
Fragments arranged by size:
891 141 137 112 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 489 1386
Size: 489 897
FspI TGC'GCA
Cuts at: 0 490 1386
Size: 490 896
HaeII r_GCGC'y
Cuts at: 0 677 1010 1031 1174 1372 1386
Size: 677 333 21 143 198 14
Fragments arranged by size:
677 333 198 143 21 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 646 678 1045 1077 1386
Size: 409 32 205 32 367 32 309
Fragments arranged by size:
409 367 309 205 32 32 32
HincII GTy'rAC
Cuts at: 0 665 1386
Size: 665 721
KasI G'GCGC_C
Cuts at: 0 1027 1368 1386
Size: 1027 341 18
Fragments arranged by size:
1027 341 18
MscI TGG'CCA
Cuts at: 0 589 979 1386
Size: 589 390 407
Fragments arranged by size:
589 407 390
MslI CAynn'nnrTG
Cuts at: 0 40 232 595 691 835 955 1081 1386
Size: 40 192 363 96 144 120 126 305
Fragments arranged by size:
363 305 192 144 126 120 96 40
MspA1I CmG'CkG
Cuts at: 0 138 452 549 1386
Size: 138 314 97 837
Fragments arranged by size:
837 314 138 97
NaeI GCC'GGC
Cuts at: 0 1268 1386
Size: 1268 118
NarI GG'CG_CC
Cuts at: 0 1028 1369 1386
Size: 1028 341 17
Fragments arranged by size:
1028 341 17
NcoI C'CATG_G
Cuts at: 0 80 212 590 1386
Size: 80 132 378 796
Fragments arranged by size:
796 378 132 80
NgoMIV G'CCGG_C
Cuts at: 0 1266 1386
Size: 1266 120
NspI r_CATG'y
Cuts at: 0 690 1080 1386
Size: 690 390 306
Fragments arranged by size:
690 390 306
PasI CC'CwG_GG
Cuts at: 0 108 252 330 1386
Size: 108 144 78 1056
Fragments arranged by size:
1056 144 108 78
PfoI T'CCnGG_A
Cuts at: 0 704 1386
Size: 704 682
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1386
Size: 760 32 594
Fragments arranged by size:
760 594 32
PpuMI rG'GwC_Cy
Cuts at: 0 354 495 1386
Size: 354 141 891
Fragments arranged by size:
891 354 141
PspOMI G'GGCC_C
Cuts at: 0 183 216 1386
Size: 183 33 1170
Fragments arranged by size:
1170 183 33
PvuII CAG'CTG
Cuts at: 0 138 452 1386
Size: 138 314 934
Fragments arranged by size:
934 314 138
SacI G_AGCT'C
Cuts at: 0 166 1386
Size: 166 1220
SalI G'TCGA_C
Cuts at: 0 663 1386
Size: 663 723
ScaI AGT'ACT
Cuts at: 0 565 1386
Size: 565 821
SexAI A'CCwGG_T
Cuts at: 0 887 1386
Size: 887 499
SfoI GGC'GCC
Cuts at: 0 1029 1370 1386
Size: 1029 341 16
Fragments arranged by size:
1029 341 16
SmaI CCC'GGG
Cuts at: 0 131 1386
Size: 131 1255
SmlI C'TyrA_G
Cuts at: 0 349 1386
Size: 349 1037
StuI AGG'CCT
Cuts at: 0 1204 1386
Size: 1204 182
StyI C'CwwG_G
Cuts at: 0 80 212 590 1139 1196 1386
Size: 80 132 378 549 57 190
Fragments arranged by size:
549 378 190 132 80 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 52 186 261 751 1350 1386
Size: 52 134 75 490 599 36
Fragments arranged by size:
599 490 134 75 52 36
TatI w'GTAC_w
Cuts at: 0 446 563 1386
Size: 446 117 823
Fragments arranged by size:
823 446 117
Tth111I GACn'n_nGTC
Cuts at: 0 790 889 1386
Size: 790 99 497
Fragments arranged by size:
790 497 99
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 177 249 1386
Size: 177 72 1137
Fragments arranged by size:
1137 177 72
XmaI C'CCGG_G
Cuts at: 0 129 1386
Size: 129 1257
Enzymes that do cut:
AarI AccI AcuI AgeI AleI ApaI AvaI BamHI
BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssHII BstYI Bsu36I BtgI BtgZI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FalI FspI HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PfoI
PpiI PpuMI PspOMI PvuII SacI SalI ScaI SexAI
SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BcgI BciVI BclI BfrBI BglII BmrI
BmtI BplI BsaBI BsiWI BsmBI BspEI BspHI BsrDI
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtsI ClaI
CspCI DraI DraIII EcoNI EcoRI EcoRV FseI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII
SanDI SapI SbfI SfcI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XhoI
XmnI ZraI