Restriction map for
pF1KB9769
(Linear) (Six-base) MAPSORT of: KIBB9769.seq Check: 5735 from: 1 to: 3141
KIBB9769.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 2579 3141
Size: 2579 562
AclI AA'CG_TT
Cuts at: 0 89 1890 3141
Size: 89 1801 1251
Fragments arranged by size:
1801 1251 89
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 958 1132 1790 1837 1931 2434 2575
Size: 593 365 174 658 47 94 503 141
Cuts at: 2575 2822 2834 3141
Size: 247 12 307
Fragments arranged by size:
658 593 503 365 307 247 174 141
94 47 12
AflIII A'CryG_T
Cuts at: 0 971 3141
Size: 971 2170
AleI CACnn'nnGTG
Cuts at: 0 1258 2209 2227 3141
Size: 1258 951 18 914
Fragments arranged by size:
1258 951 914 18
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2200 2232 3141
Size: 2200 32 909
Fragments arranged by size:
2200 909 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1246 2821 3141
Size: 1246 1575 320
Fragments arranged by size:
1575 1246 320
ApaLI G'TGCA_C
Cuts at: 0 3070 3141
Size: 3070 71
ApoI r'AATT_y
Cuts at: 0 421 586 1063 2343 2386 2658 3141
Size: 421 165 477 1280 43 272 483
Fragments arranged by size:
1280 483 477 421 272 165 43
AvaI C'yCGr_G
Cuts at: 0 1364 1901 3141
Size: 1364 537 1240
Fragments arranged by size:
1364 1240 537
AvrII C'CTAG_G
Cuts at: 0 1041 3141
Size: 1041 2100
BanI G'GyrC_C
Cuts at: 0 1818 3141
Size: 1818 1323
BanII G_rGCy'C
Cuts at: 0 300 539 1078 2427 3141
Size: 300 239 539 1349 714
Fragments arranged by size:
1349 714 539 300 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 570 2725 3141
Size: 570 2155 416
Fragments arranged by size:
2155 570 416
BclI T'GATC_A
Cuts at: 0 1955 2609 3141
Size: 1955 654 532
Fragments arranged by size:
1955 654 532
BfrBI ATG'CAT
Cuts at: 0 2271 3141
Size: 2271 870
BglII A'GATC_T
Cuts at: 0 1583 1989 3141
Size: 1583 406 1152
Fragments arranged by size:
1583 1152 406
BlpI GC'TnA_GC
Cuts at: 0 2294 3141
Size: 2294 847
Bme1580I G_kGCm'C
Cuts at: 0 3074 3141
Size: 3074 67
BmgBI CAC'GTC
Cuts at: 0 972 993 3141
Size: 972 21 2148
Fragments arranged by size:
2148 972 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2302 3141
Size: 2302 839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 901 2059 2794 2998 3141
Size: 449 452 1158 735 204 143
Fragments arranged by size:
1158 735 452 449 204 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 869 2155 3141
Size: 748 121 1286 986
Fragments arranged by size:
1286 986 748 121
BsaBI GATnn'nnATC
Cuts at: 0 1588 3141
Size: 1588 1553
BsaWI w'CCGG_w
Cuts at: 0 1967 3141
Size: 1967 1174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2072 2102 3141
Size: 2072 30 1039
Fragments arranged by size:
2072 1039 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 943 1333 1336 1339 2093 2890 3141
Size: 943 390 3 3 754 797 251
Fragments arranged by size:
943 797 754 390 251 3 3
BseYI C'CCAG_C
Cuts at: 0 694 1239 3141
Size: 694 545 1902
Fragments arranged by size:
1902 694 545
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 2143 2233 3101 3141
Size: 515 1628 90 868 40
Fragments arranged by size:
1628 868 515 90 40
BsiHKAI G_wGCw'C
Cuts at: 0 539 1428 1734 2427 3074 3141
Size: 539 889 306 693 647 67
Fragments arranged by size:
889 693 647 539 306 67
BsmI GAATG_Cn'
Cuts at: 0 291 1863 2181 3141
Size: 291 1572 318 960
Fragments arranged by size:
1572 960 318 291
Bsp1286I G_dGCh'C
Cuts at: 0 300 539 1078 1428 1734 2427 3074 3141
Size: 300 239 539 350 306 693 647 67
Fragments arranged by size:
693 647 539 350 306 300 239 67
BspHI T'CATG_A
Cuts at: 0 437 2714 3141
Size: 437 2277 427
Fragments arranged by size:
2277 437 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2449 3141
Size: 2449 692
BsrBI CCG'CTC
Cuts at: 0 2538 3141
Size: 2538 603
BsrDI GCAATG_nn'
Cuts at: 0 285 1631 1649 3141
Size: 285 1346 18 1492
Fragments arranged by size:
1492 1346 285 18
BsrGI T'GTAC_A
Cuts at: 0 50 2099 3141
Size: 50 2049 1042
Fragments arranged by size:
2049 1042 50
BstBI TT'CG_AA
Cuts at: 0 2750 3141
Size: 2750 391
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2053 2250 3141
Size: 2053 197 891
Fragments arranged by size:
2053 891 197
BstYI r'GATC_y
Cuts at: 0 1583 1989 3141
Size: 1583 406 1152
Fragments arranged by size:
1583 1152 406
Bsu36I CC'TnA_GG
Cuts at: 0 1556 2738 2780 3141
Size: 1556 1182 42 361
Fragments arranged by size:
1556 1182 361 42
BtgI C'CryG_G
Cuts at: 0 821 1679 2243 3084 3096 3141
Size: 821 858 564 841 12 45
Fragments arranged by size:
858 841 821 564 45 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 498 3141
Size: 498 2643
BtsI GCAGTG_nn'
Cuts at: 0 310 1734 3141
Size: 310 1424 1407
Fragments arranged by size:
1424 1407 310
ClaI AT'CG_AT
Cuts at: 0 1592 3141
Size: 1592 1549
DraI TTT'AAA
Cuts at: 0 2340 3141
Size: 2340 801
EaeI y'GGCC_r
Cuts at: 0 227 2488 3141
Size: 227 2261 653
Fragments arranged by size:
2261 653 227
EarI CTCTTCn'nnn_
Cuts at: 0 37 436 671 1178 1310 1350 1406 1416
Size: 37 399 235 507 132 40 56 10
Cuts at: 1416 1907 2861 2867 2906 2945 3049 3141
Size: 491 954 6 39 39 104 92
Fragments arranged by size:
954 507 491 399 235 132 104 92
56 40 39 39 37 10 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 78 3141
Size: 78 3063
EcoICRI GAG'CTC
Cuts at: 0 537 2425 3141
Size: 537 1888 716
Fragments arranged by size:
1888 716 537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 593 901 958 1132 1790 1837 1931
Size: 449 144 308 57 174 658 47 94
Cuts at: 1931 2059 2434 2575 2794 2822 2834 2998 3141
Size: 128 375 141 219 28 12 164 143
Fragments arranged by size:
658 449 375 308 219 174 164 144
143 141 128 94 57 47 28 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1344 3141
Size: 1344 1797
EcoRI G'AATT_C
Cuts at: 0 1063 2658 3141
Size: 1063 1595 483
Fragments arranged by size:
1595 1063 483
EcoRV GAT'ATC
Cuts at: 0 489 3141
Size: 489 2652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 366 3141
Size: 366 2775
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 861 893 2803 2829 2835 2861 2884 2916
Size: 861 32 1910 26 6 26 23 32
Cuts at: 2916 3141
Size: 225
Fragments arranged by size:
1910 861 225 32 32 26 26 23
6
MfeI C'AATT_G
Cuts at: 0 57 3141
Size: 57 3084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 3141
Size: 875 2266
MscI TGG'CCA
Cuts at: 0 229 2490 3141
Size: 229 2261 651
Fragments arranged by size:
2261 651 229
MslI CAynn'nnrTG
Cuts at: 0 223 868 991 1258 2051 2207 2209 2227
Size: 223 645 123 267 793 156 2 18
Cuts at: 2227 2270 3141
Size: 43 871
Fragments arranged by size:
871 793 645 267 223 156 123 43
18 2
NcoI C'CATG_G
Cuts at: 0 821 3141
Size: 821 2320
NdeI CA'TA_TG
Cuts at: 0 794 2267 3141
Size: 794 1473 874
Fragments arranged by size:
1473 874 794
NsiI A_TGCA'T
Cuts at: 0 2273 3141
Size: 2273 868
NspI r_CATG'y
Cuts at: 0 2119 3141
Size: 2119 1022
PfoI T'CCnGG_A
Cuts at: 0 2771 3141
Size: 2771 370
PpuMI rG'GwC_Cy
Cuts at: 0 1344 3141
Size: 1344 1797
PshAI GACnn'nnGTC
Cuts at: 0 1780 3141
Size: 1780 1361
PsiI TTA'TAA
Cuts at: 0 905 1895 3141
Size: 905 990 1246
Fragments arranged by size:
1246 990 905
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1820 1852 3141
Size: 1820 32 1289
Fragments arranged by size:
1820 1289 32
PstI C_TGCA'G
Cuts at: 0 1410 3141
Size: 1410 1731
RsrII CG'GwC_CG
Cuts at: 0 3000 3141
Size: 3000 141
SacI G_AGCT'C
Cuts at: 0 539 2427 3141
Size: 539 1888 714
Fragments arranged by size:
1888 714 539
SapI GCTCTTCn'nnn_
Cuts at: 0 436 1416 3141
Size: 436 980 1725
Fragments arranged by size:
1725 980 436
SfcI C'TryA_G
Cuts at: 0 1406 2430 2589 2624 3141
Size: 1406 1024 159 35 517
Fragments arranged by size:
1406 1024 517 159 35
SmlI C'TyrA_G
Cuts at: 0 763 884 1901 2170 3141
Size: 763 121 1017 269 971
Fragments arranged by size:
1017 971 763 269 121
SpeI A'CTAG_T
Cuts at: 0 2458 3141
Size: 2458 683
SphI G_CATG'C
Cuts at: 0 2119 3141
Size: 2119 1022
SspI AAT'ATT
Cuts at: 0 1812 2010 2109 3141
Size: 1812 198 99 1032
Fragments arranged by size:
1812 1032 198 99
StyI C'CwwG_G
Cuts at: 0 821 1041 3141
Size: 821 220 2100
Fragments arranged by size:
2100 821 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 2291 3017 3141
Size: 103 2188 726 124
Fragments arranged by size:
2188 726 124 103
TatI w'GTAC_w
Cuts at: 0 50 1881 2099 2688 3141
Size: 50 1831 218 589 453
Fragments arranged by size:
1831 589 453 218 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2072 2104 3141
Size: 2072 32 1037
Fragments arranged by size:
2072 1037 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2134 3141
Size: 2134 1007
XhoI C'TCGA_G
Cuts at: 0 1901 3141
Size: 1901 1240
XmnI GAAnn'nnTTC
Cuts at: 0 1359 1861 3141
Size: 1359 502 1280
Fragments arranged by size:
1359 1280 502
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AloI AlwNI ApaLI
ApoI AvaI AvrII BanI BanII BbsI BclI BfrBI
BglII BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrGI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
MfeI MmeI MscI MslI NcoI NdeI NsiI NspI
PfoI PpuMI PshAI PsiI PsrI PstI RsrII SacI
SapI SfcI SmlI SpeI SphI SspI StyI TaqII
TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI
ApaI AscI AseI AsiSI BaeI BamHI BbeI BbvCI
BcgI BciVI BglI BmrI BmtI Bpu10I BsaI BsaAI
BsaHI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI
BstAPI BstEII BstZ17I CspCI DraIII DrdI EagI EcoNI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MspA1I NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PspOMI PspXI PvuI PvuII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI StuI SwaI Tth111I XbaI XmaI ZraI