Restriction map for
pF1KB9762
(Linear) (Six-base) MAPSORT of: KIBB9762.seq Check: 5346 from: 1 to: 2400
KIBB9762.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AatII G_ACGT'C
Cuts at: 0 1967 2400
Size: 1967 433
AccI GT'mk_AC
Cuts at: 0 755 1637 2400
Size: 755 882 763
Fragments arranged by size:
882 763 755
Acc65I G'GTAC_C
Cuts at: 0 1149 2400
Size: 1149 1251
AclI AA'CG_TT
Cuts at: 0 149 2400
Size: 149 2251
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1635 1667 2400
Size: 946 689 32 733
Fragments arranged by size:
946 733 689 32
AflIII A'CryG_T
Cuts at: 0 737 2400
Size: 737 1663
AleI CACnn'nnGTG
Cuts at: 0 1423 2400
Size: 1423 977
AlwNI CAG_nnn'CTG
Cuts at: 0 541 2266 2400
Size: 541 1725 134
Fragments arranged by size:
1725 541 134
ApaLI G'TGCA_C
Cuts at: 0 733 2400
Size: 733 1667
ApoI r'AATT_y
Cuts at: 0 910 1911 2171 2277 2289 2400
Size: 910 1001 260 106 12 111
Fragments arranged by size:
1001 910 260 111 106 12
AvaI C'yCGr_G
Cuts at: 0 396 2400
Size: 396 2004
BamHI G'GATC_C
Cuts at: 0 1231 2400
Size: 1231 1169
BanI G'GyrC_C
Cuts at: 0 240 1149 2400
Size: 240 909 1251
Fragments arranged by size:
1251 909 240
BanII G_rGCy'C
Cuts at: 0 388 396 1936 2400
Size: 388 8 1540 464
Fragments arranged by size:
1540 464 388 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 2400
Size: 584 1816
BbvCI CC'TCA_GC
Cuts at: 0 422 2400
Size: 422 1978
BglI GCCn_nnn'nGGC
Cuts at: 0 134 340 2400
Size: 134 206 2060
Fragments arranged by size:
2060 206 134
BglII A'GATC_T
Cuts at: 0 1533 2400
Size: 1533 867
Bme1580I G_kGCm'C
Cuts at: 0 245 496 737 2400
Size: 245 251 241 1663
Fragments arranged by size:
1663 251 245 241
BmrI ACTGGGnnnn_n'
Cuts at: 0 2276 2400
Size: 2276 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1478 2400
Size: 388 1090 922
Fragments arranged by size:
1090 922 388
Bpu10I CC'TnA_GC
Cuts at: 0 422 2400
Size: 422 1978
BsaI GGTCTCn'nnnn_
Cuts at: 0 877 1420 2236 2400
Size: 877 543 816 164
Fragments arranged by size:
877 816 543 164
BsaHI Gr'CG_yC
Cuts at: 0 157 260 1964 2400
Size: 157 103 1704 436
Fragments arranged by size:
1704 436 157 103
BsaWI w'CCGG_w
Cuts at: 0 255 388 1656 2400
Size: 255 133 1268 744
Fragments arranged by size:
1268 744 255 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2016 2046 2290 2320 2400
Size: 2016 30 244 30 80
Fragments arranged by size:
2016 244 80 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 385 2400
Size: 31 354 2015
Fragments arranged by size:
2015 354 31
BsiEI CG_ry'CG
Cuts at: 0 518 2400
Size: 518 1882
BsiHKAI G_wGCw'C
Cuts at: 0 737 1007 2400
Size: 737 270 1393
Fragments arranged by size:
1393 737 270
BsmBI CGTCTCn'nnnn_
Cuts at: 0 525 2400
Size: 525 1875
Bsp1286I G_dGCh'C
Cuts at: 0 245 388 396 496 737 1007 1936 2400
Size: 245 143 8 100 241 270 929 464
Fragments arranged by size:
929 464 270 245 241 143 100 8
BspEI T'CCGG_A
Cuts at: 0 255 388 1656 2400
Size: 255 133 1268 744
Fragments arranged by size:
1268 744 255 133
BsrFI r'CCGG_y
Cuts at: 0 262 415 1870 2400
Size: 262 153 1455 530
Fragments arranged by size:
1455 530 262 153
BsrGI T'GTAC_A
Cuts at: 0 2340 2400
Size: 2340 60
BssHII G'CGCG_C
Cuts at: 0 434 2400
Size: 434 1966
BstBI TT'CG_AA
Cuts at: 0 900 2400
Size: 900 1500
BstEII G'GTnAC_C
Cuts at: 0 426 450 2400
Size: 426 24 1950
Fragments arranged by size:
1950 426 24
BstYI r'GATC_y
Cuts at: 0 627 1231 1251 1533 1988 2400
Size: 627 604 20 282 455 412
Fragments arranged by size:
627 604 455 412 282 20
Bsu36I CC'TnA_GG
Cuts at: 0 50 534 2400
Size: 50 484 1866
Fragments arranged by size:
1866 484 50
BtgI C'CryG_G
Cuts at: 0 197 512 801 2400
Size: 197 315 289 1599
Fragments arranged by size:
1599 315 289 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1075 2400
Size: 1075 1325
BtsI GCAGTG_nn'
Cuts at: 0 383 1418 1430 2400
Size: 383 1035 12 970
Fragments arranged by size:
1035 970 383 12
EaeI y'GGCC_r
Cuts at: 0 293 1872 2400
Size: 293 1579 528
Fragments arranged by size:
1579 528 293
EarI CTCTTCn'nnn_
Cuts at: 0 332 946 1166 1289 1686 2400
Size: 332 614 220 123 397 714
Fragments arranged by size:
714 614 397 332 220 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 201 2400
Size: 28 173 2199
Fragments arranged by size:
2199 173 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 946 1478 1635 1667 2400
Size: 388 558 532 157 32 733
Fragments arranged by size:
733 558 532 388 157 32
EcoO109I rG'GnC_Cy
Cuts at: 0 185 538 2400
Size: 185 353 1862
Fragments arranged by size:
1862 353 185
EcoRI G'AATT_C
Cuts at: 0 2289 2400
Size: 2289 111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 2400
Size: 804 1596
HaeII r_GCGC'y
Cuts at: 0 10 269 346 416 422 503 2400
Size: 10 259 77 70 6 81 1897
Fragments arranged by size:
1897 259 81 77 70 10 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1540 1572 2016 2048 2400
Size: 1540 32 444 32 352
Fragments arranged by size:
1540 444 352 32 32
HindIII A'AGCT_T
Cuts at: 0 1507 2400
Size: 1507 893
KpnI G_GTAC'C
Cuts at: 0 1153 2400
Size: 1153 1247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 872 1292 2091 2400
Size: 540 332 420 799 309
Fragments arranged by size:
799 540 420 332 309
MslI CAynn'nnrTG
Cuts at: 0 770 1423 2400
Size: 770 653 977
Fragments arranged by size:
977 770 653
MspA1I CmG'CkG
Cuts at: 0 217 250 2400
Size: 217 33 2150
Fragments arranged by size:
2150 217 33
NaeI GCC'GGC
Cuts at: 0 264 417 1872 2400
Size: 264 153 1455 528
Fragments arranged by size:
1455 528 264 153
NcoI C'CATG_G
Cuts at: 0 801 2400
Size: 801 1599
NdeI CA'TA_TG
Cuts at: 0 1785 2400
Size: 1785 615
NgoMIV G'CCGG_C
Cuts at: 0 262 415 1870 2400
Size: 262 153 1455 530
Fragments arranged by size:
1455 530 262 153
NspI r_CATG'y
Cuts at: 0 741 2400
Size: 741 1659
PciI A'CATG_T
Cuts at: 0 737 2400
Size: 737 1663
PpuMI rG'GwC_Cy
Cuts at: 0 185 538 2400
Size: 185 353 1862
Fragments arranged by size:
1862 353 185
PstI C_TGCA'G
Cuts at: 0 305 1425 1941 2382 2400
Size: 305 1120 516 441 18
Fragments arranged by size:
1120 516 441 305 18
SanDI GG'GwC_CC
Cuts at: 0 185 2400
Size: 185 2215
SexAI A'CCwGG_T
Cuts at: 0 2366 2400
Size: 2366 34
SfcI C'TryA_G
Cuts at: 0 301 651 1421 1838 1937 2054 2258 2378
Size: 301 350 770 417 99 117 204 120
Cuts at: 2378 2400
Size: 22
Fragments arranged by size:
770 417 350 301 204 120 117 99
22
SgrAI Cr'CCGG_yG
Cuts at: 0 262 415 2400
Size: 262 153 1985
Fragments arranged by size:
1985 262 153
SmaI CCC'GGG
Cuts at: 0 398 2400
Size: 398 2002
SspI AAT'ATT
Cuts at: 0 1865 2400
Size: 1865 535
StyI C'CwwG_G
Cuts at: 0 801 1356 2400
Size: 801 555 1044
Fragments arranged by size:
1044 801 555
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 293 1515 1832 2260 2400
Size: 293 1222 317 428 140
Fragments arranged by size:
1222 428 317 293 140
TatI w'GTAC_w
Cuts at: 0 740 2340 2400
Size: 740 1600 60
Fragments arranged by size:
1600 740 60
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 2400
Size: 198 2202
XmaI C'CCGG_G
Cuts at: 0 396 2400
Size: 396 2004
XmnI GAAnn'nnTTC
Cuts at: 0 667 2400
Size: 667 1733
ZraI GAC'GTC
Cuts at: 0 1965 2400
Size: 1965 435
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BglI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BsrFI BsrGI BssHII BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HindIII KpnI MmeI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PciI PpuMI PstI
SanDI SexAI SfcI SgrAI SmaI SspI StyI TaqII
TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaBI BseYI BsgI BsiWI BsmI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoRV FseI FspI
FspAI HincII HpaI KasI MfeI MluI MscI NarI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SgfI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XhoI