Restriction map for
pF1KB9743
(Linear) (Six-base) MAPSORT of: KIBB9743.seq Check: 6519 from: 1 to: 1572
KIBB9743.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 437 1572
Size: 437 1135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 769 1572
Size: 49 720 803
Fragments arranged by size:
803 720 49
AfeI AGC'GCT
Cuts at: 0 817 907 1572
Size: 817 90 665
Fragments arranged by size:
817 665 90
AgeI A'CCGG_T
Cuts at: 0 761 1572
Size: 761 811
AleI CACnn'nnGTG
Cuts at: 0 773 1572
Size: 773 799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 449 1572
Size: 449 1123
AlwNI CAG_nnn'CTG
Cuts at: 0 357 1572
Size: 357 1215
ApaI G_GGCC'C
Cuts at: 0 423 1053 1198 1365 1572
Size: 423 630 145 167 207
Fragments arranged by size:
630 423 207 167 145
AvaI C'yCGr_G
Cuts at: 0 196 298 586 1572
Size: 196 102 288 986
Fragments arranged by size:
986 288 196 102
BanI G'GyrC_C
Cuts at: 0 1350 1453 1572
Size: 1350 103 119
Fragments arranged by size:
1350 119 103
BanII G_rGCy'C
Cuts at: 0 423 542 983 1053 1198 1365 1543 1572
Size: 423 119 441 70 145 167 178 29
Fragments arranged by size:
441 423 178 167 145 119 70 29
BbvCI CC'TCA_GC
Cuts at: 0 241 1572
Size: 241 1331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 577 611 1572
Size: 577 34 961
Fragments arranged by size:
961 577 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1103 1572
Size: 1103 469
Bme1580I G_kGCm'C
Cuts at: 0 335 423 1053 1149 1198 1355 1365 1572
Size: 335 88 630 96 49 157 10 207
Fragments arranged by size:
630 335 207 157 96 88 49 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 329 624 1285 1572
Size: 329 295 661 287
Fragments arranged by size:
661 329 295 287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 311 760 813 1572
Size: 137 174 449 53 759
Fragments arranged by size:
759 449 174 137 53
Bpu10I CC'TnA_GC
Cuts at: 0 241 1572
Size: 241 1331
BsaI GGTCTCn'nnnn_
Cuts at: 0 1296 1572
Size: 1296 276
BsaAI yAC'GTr
Cuts at: 0 864 1572
Size: 864 708
BsaBI GATnn'nnATC
Cuts at: 0 667 1060 1572
Size: 667 393 512
Fragments arranged by size:
667 512 393
BsaHI Gr'CG_yC
Cuts at: 0 1387 1478 1572
Size: 1387 91 94
Fragments arranged by size:
1387 94 91
BsaWI w'CCGG_w
Cuts at: 0 761 1572
Size: 761 811
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 429 1572
Size: 429 1143
BseYI C'CCAG_C
Cuts at: 0 81 117 379 512 919 1071 1520 1572
Size: 81 36 262 133 407 152 449 52
Fragments arranged by size:
449 407 262 152 133 81 52 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 409 1572
Size: 91 318 1163
Fragments arranged by size:
1163 318 91
BsiEI CG_ry'CG
Cuts at: 0 766 1572
Size: 766 806
BsiHKAI G_wGCw'C
Cuts at: 0 105 542 983 1572
Size: 105 437 441 589
Fragments arranged by size:
589 441 437 105
Bsp1286I G_dGCh'C
Cuts at: 0 105 335 423 542 983 1053 1149 1198
Size: 105 230 88 119 441 70 96 49
Cuts at: 1198 1355 1365 1543 1572
Size: 157 10 178 29
Fragments arranged by size:
441 230 178 157 119 105 96 88
70 49 29 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 437 491 724 1572
Size: 329 108 54 233 848
Fragments arranged by size:
848 329 233 108 54
BsrBI CCG'CTC
Cuts at: 0 21 1572
Size: 21 1551
BsrFI r'CCGG_y
Cuts at: 0 761 1557 1572
Size: 761 796 15
Fragments arranged by size:
796 761 15
BssSI C'ACGA_G
Cuts at: 0 232 967 1572
Size: 232 735 605
Fragments arranged by size:
735 605 232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 1365 1572
Size: 501 864 207
Fragments arranged by size:
864 501 207
BstYI r'GATC_y
Cuts at: 0 752 1572
Size: 752 820
Bsu36I CC'TnA_GG
Cuts at: 0 168 1572
Size: 168 1404
BtgI C'CryG_G
Cuts at: 0 633 948 1472 1572
Size: 633 315 524 100
Fragments arranged by size:
633 524 315 100
BtsI GCAGTG_nn'
Cuts at: 0 355 925 1012 1179 1572
Size: 355 570 87 167 393
Fragments arranged by size:
570 393 355 167 87
DraIII CAC_nnn'GTG
Cuts at: 0 864 960 1572
Size: 864 96 612
Fragments arranged by size:
864 612 96
EaeI y'GGCC_r
Cuts at: 0 636 951 1232 1420 1572
Size: 636 315 281 188 152
Fragments arranged by size:
636 315 281 188 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1286 1572
Size: 1286 286
EcoICRI GAG'CTC
Cuts at: 0 540 981 1572
Size: 540 441 591
Fragments arranged by size:
591 540 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 137 311 760 769 813 1572
Size: 49 88 174 449 9 44 759
Fragments arranged by size:
759 449 174 88 49 44 9
EcoO109I rG'GnC_Cy
Cuts at: 0 318 419 420 1049 1090 1195 1210 1361
Size: 318 101 1 629 41 105 15 151
Cuts at: 1361 1362 1572
Size: 1 210
Fragments arranged by size:
629 318 210 151 105 101 41 15
1 1
FspI TGC'GCA
Cuts at: 0 931 1572
Size: 931 641
HaeII r_GCGC'y
Cuts at: 0 306 819 909 1572
Size: 306 513 90 663
Fragments arranged by size:
663 513 306 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 907 939 1572
Size: 907 32 633
Fragments arranged by size:
907 633 32
HincII GTy'rAC
Cuts at: 0 567 1482 1572
Size: 567 915 90
Fragments arranged by size:
915 567 90
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 848 1572
Size: 736 112 724
Fragments arranged by size:
736 724 112
MscI TGG'CCA
Cuts at: 0 953 1234 1572
Size: 953 281 338
Fragments arranged by size:
953 338 281
MslI CAynn'nnrTG
Cuts at: 0 773 1572
Size: 773 799
MspA1I CmG'CkG
Cuts at: 0 501 707 990 1075 1245 1312 1412 1489
Size: 501 206 283 85 170 67 100 77
Cuts at: 1489 1572
Size: 83
Fragments arranged by size:
501 283 206 170 100 85 83 77
67
NcoI C'CATG_G
Cuts at: 0 633 948 1572
Size: 633 315 624
Fragments arranged by size:
633 624 315
PasI CC'CwG_GG
Cuts at: 0 1198 1365 1572
Size: 1198 167 207
Fragments arranged by size:
1198 207 167
PflMI CCAn_nnn'nTGG
Cuts at: 0 259 864 1572
Size: 259 605 708
Fragments arranged by size:
708 605 259
PpuMI rG'GwC_Cy
Cuts at: 0 318 1572
Size: 318 1254
PspOMI G'GGCC_C
Cuts at: 0 419 1049 1194 1361 1572
Size: 419 630 145 167 211
Fragments arranged by size:
630 419 211 167 145
PstI C_TGCA'G
Cuts at: 0 721 989 1572
Size: 721 268 583
Fragments arranged by size:
721 583 268
PvuII CAG'CTG
Cuts at: 0 501 990 1075 1312 1412 1489 1572
Size: 501 489 85 237 100 77 83
Fragments arranged by size:
501 489 237 100 85 83 77
SacI G_AGCT'C
Cuts at: 0 542 983 1572
Size: 542 441 589
Fragments arranged by size:
589 542 441
SexAI A'CCwGG_T
Cuts at: 0 551 1572
Size: 551 1021
SfcI C'TryA_G
Cuts at: 0 717 985 1572
Size: 717 268 587
Fragments arranged by size:
717 587 268
StyI C'CwwG_G
Cuts at: 0 458 633 948 1572
Size: 458 175 315 624
Fragments arranged by size:
624 458 315 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 871 1512 1572
Size: 73 798 641 60
Fragments arranged by size:
798 641 73 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 69 101 946 978 1572
Size: 69 32 845 32 594
Fragments arranged by size:
845 594 69 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 562 1572
Size: 562 1010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1362 1572
Size: 1362 210
Enzymes that do cut:
AarI AcuI AfeI AgeI AleI AlfI AlwNI ApaI
AvaI BanI BanII BbvCI BcgI BglI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI PasI PflMI PpuMI
PspOMI PstI PvuII SacI SexAI SfcI StyI TaqII
TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BpuEI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EarI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI XbaI XhoI XmaI XmnI
ZraI