Restriction map for pF1KB9702
 (Linear) (Six-base) MAPSORT of: KIBB9702.seq  Check: 3089  from: 1  to: 981



KIBB9702.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     239     981 
   Size:        239     742
 
Acc65I G'GTAC_C

Cuts at:      0     674     981 
   Size:        674     307
 
AfeI AGC'GCT

Cuts at:      0     755     981 
   Size:        755     226
 
AlwNI CAG_nnn'CTG

Cuts at:      0     140     367     798     981 
   Size:        140     227     431     183

  Fragments arranged by size: 

                 431     227     183     140
 
ApaLI G'TGCA_C

Cuts at:      0     332     981 
   Size:        332     649
 
AvaI C'yCGr_G

Cuts at:      0      85     238     981 
   Size:         85     153     743

  Fragments arranged by size: 

                 743     153      85
 
BanI G'GyrC_C

Cuts at:      0     674     882     913     981 
   Size:        674     208      31      68

  Fragments arranged by size: 

                 674     208      68      31
 
BanII G_rGCy'C

Cuts at:      0     632     981 
   Size:        632     349
 
BbeI G_GCGC'C

Cuts at:      0     917     981 
   Size:        917      64
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     303     337     612     646     981 
   Size:        303      34     275      34     335

  Fragments arranged by size: 

                 335     303     275      34      34
 
BclI T'GATC_A

Cuts at:      0     290     981 
   Size:        290     691
 
BglII A'GATC_T

Cuts at:      0     488     981 
   Size:        488     493
 
Bme1580I G_kGCm'C

Cuts at:      0     336     887     981 
   Size:        336     551      94

  Fragments arranged by size: 

                 551     336      94
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     255     981 
   Size:        255     726
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     143     477     483     598     742     853     892 
   Size:        127      16     334       6     115     144     111      39

Cuts at:    892     981 
   Size:         89

  Fragments arranged by size: 

                 334     144     127     115     111      89      39      16
                   6
 
Bpu10I CC'TnA_GC

Cuts at:      0     368     607     981 
   Size:        368     239     374

  Fragments arranged by size: 

                 374     368     239
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     867     981 
   Size:        867     114
 
BsaHI Gr'CG_yC

Cuts at:      0     914     953     981 
   Size:        914      39      28

  Fragments arranged by size: 

                 914      39      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     270     487     981 
   Size:        270     217     494

  Fragments arranged by size: 

                 494     270     217
 
BseYI C'CCAG_C

Cuts at:      0     847     981 
   Size:        847     134
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     634     981 
   Size:        634     347
 
BsiEI CG_ry'CG

Cuts at:      0     483     713     981 
   Size:        483     230     268

  Fragments arranged by size: 

                 483     268     230
 
BsiHKAI G_wGCw'C

Cuts at:      0     336     632     981 
   Size:        336     296     349

  Fragments arranged by size: 

                 349     336     296
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     172     981 
   Size:        172     809
 
Bsp1286I G_dGCh'C

Cuts at:      0     336     632     887     981 
   Size:        336     296     255      94

  Fragments arranged by size: 

                 336     296     255      94
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     239     651     981 
   Size:        239     412     330

  Fragments arranged by size: 

                 412     330     239
 
BsrBI CCG'CTC

Cuts at:      0     594     981 
   Size:        594     387
 
BsrFI r'CCGG_y

Cuts at:      0     357     949     981 
   Size:        357     592      32

  Fragments arranged by size: 

                 592     357      32
 
BssHII G'CGCG_C

Cuts at:      0     541     851     981 
   Size:        541     310     130

  Fragments arranged by size: 

                 541     310     130
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     140     981 
   Size:        140     841
 
BstYI r'GATC_y

Cuts at:      0     488     981 
   Size:        488     493
 
BtgI C'CryG_G

Cuts at:      0     190     398     840     900     981 
   Size:        190     208     442      60      81

  Fragments arranged by size: 

                 442     208     190      81      60
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     661     981 
   Size:        661     320
 
EaeI y'GGCC_r

Cuts at:      0     389     710     947     981 
   Size:        389     321     237      34

  Fragments arranged by size: 

                 389     321     237      34
 
EagI C'GGCC_G

Cuts at:      0     710     981 
   Size:        710     271
 
EarI CTCTTCn'nnn_

Cuts at:      0     150     981 
   Size:        150     831
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     748     981 
   Size:        748     233
 
EcoICRI GAG'CTC

Cuts at:      0     630     981 
   Size:        630     351
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     143     477     483     598     742     853     892 
   Size:        127      16     334       6     115     144     111      39

Cuts at:    892     981 
   Size:         89

  Fragments arranged by size: 

                 334     144     127     115     111      89      39      16
                   6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      67     981 
   Size:         67     914
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     185     981 
   Size:         75     110     796

  Fragments arranged by size: 

                 796     110      75
 
HaeII r_GCGC'y

Cuts at:      0     757     829     917     981 
   Size:        757      72      88      64

  Fragments arranged by size: 

                 757      88      72      64
 
KasI G'GCGC_C

Cuts at:      0     913     981 
   Size:        913      68
 
KpnI G_GTAC'C

Cuts at:      0     678     981 
   Size:        678     303
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     701     830     981 
   Size:        701     129     151

  Fragments arranged by size: 

                 701     151     129
 
MspA1I CmG'CkG

Cuts at:      0     350     400     567     576     762     842     936     981 
   Size:        350      50     167       9     186      80      94      45

  Fragments arranged by size: 

                 350     186     167      94      80      50      45       9
            
 
NaeI GCC'GGC

Cuts at:      0     359     951     981 
   Size:        359     592      30

  Fragments arranged by size: 

                 592     359      30
 
NarI GG'CG_CC

Cuts at:      0     914     981 
   Size:        914      67
 
NgoMIV G'CCGG_C

Cuts at:      0     357     949     981 
   Size:        357     592      32

  Fragments arranged by size: 

                 592     357      32
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     196     567     981 
   Size:        196     371     414

  Fragments arranged by size: 

                 414     371     196
 
PfoI T'CCnGG_A

Cuts at:      0     159     981 
   Size:        159     822
 
PpuMI rG'GwC_Cy

Cuts at:      0      75     185     981 
   Size:         75     110     796

  Fragments arranged by size: 

                 796     110      75
 
PshAI GACnn'nnGTC

Cuts at:      0     712     981 
   Size:        712     269
 
PstI C_TGCA'G

Cuts at:      0     236     981 
   Size:        236     745
 
PvuII CAG'CTG

Cuts at:      0     567     576     981 
   Size:        567       9     405

  Fragments arranged by size: 

                 567     405       9
 
SacI G_AGCT'C

Cuts at:      0     632     981 
   Size:        632     349
 
SacII CC_GC'GG

Cuts at:      0     401     843     981 
   Size:        401     442     138

  Fragments arranged by size: 

                 442     401     138
 
SanDI GG'GwC_CC

Cuts at:      0      75     185     981 
   Size:         75     110     796

  Fragments arranged by size: 

                 796     110      75
 
SapI GCTCTTCn'nnn_

Cuts at:      0     150     981 
   Size:        150     831
 
SexAI A'CCwGG_T

Cuts at:      0     670     981 
   Size:        670     311
 
SfcI C'TryA_G

Cuts at:      0     232     981 
   Size:        232     749
 
SfoI GGC'GCC

Cuts at:      0     915     981 
   Size:        915      66
 
SmaI CCC'GGG

Cuts at:      0     240     981 
   Size:        240     741
 
SrfI GCCC'GGGC

Cuts at:      0     240     981 
   Size:        240     741
 
StyI C'CwwG_G

Cuts at:      0      68     392     981 
   Size:         68     324     589

  Fragments arranged by size: 

                 589     324      68
 
XmaI C'CCGG_G

Cuts at:      0     238     981 
   Size:        238     743
 

 Enzymes that do cut:

     AarI   Acc65I     AfeI    AlwNI    ApaLI     AvaI     BanI    BanII
     BbeI     BcgI     BclI    BglII Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstYI     BtgI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII     KasI     KpnI     MmeI   MspA1I     NaeI     NarI
   NgoMIV    PflMI     PfoI    PpuMI    PshAI     PstI    PvuII     SacI
    SacII    SanDI     SapI    SexAI     SfcI     SfoI     SmaI     SrfI
     StyI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI
    BsaXI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     MfeI     MluI     MscI
     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SalI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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