(Linear) (Six-base) MAPSORT of: KIBB9702.seq Check: 3089 from: 1 to: 981 KIBB9702.orf With 182 enzymes: SgfI * March 3, 2008 11:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 239 981 Size: 239 742 Acc65I G'GTAC_C Cuts at: 0 674 981 Size: 674 307 AfeI AGC'GCT Cuts at: 0 755 981 Size: 755 226 AlwNI CAG_nnn'CTG Cuts at: 0 140 367 798 981 Size: 140 227 431 183 Fragments arranged by size: 431 227 183 140 ApaLI G'TGCA_C Cuts at: 0 332 981 Size: 332 649 AvaI C'yCGr_G Cuts at: 0 85 238 981 Size: 85 153 743 Fragments arranged by size: 743 153 85 BanI G'GyrC_C Cuts at: 0 674 882 913 981 Size: 674 208 31 68 Fragments arranged by size: 674 208 68 31 BanII G_rGCy'C Cuts at: 0 632 981 Size: 632 349 BbeI G_GCGC'C Cuts at: 0 917 981 Size: 917 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 303 337 612 646 981 Size: 303 34 275 34 335 Fragments arranged by size: 335 303 275 34 34 BclI T'GATC_A Cuts at: 0 290 981 Size: 290 691 BglII A'GATC_T Cuts at: 0 488 981 Size: 488 493 Bme1580I G_kGCm'C Cuts at: 0 336 887 981 Size: 336 551 94 Fragments arranged by size: 551 336 94 BmrI ACTGGGnnnn_n' Cuts at: 0 255 981 Size: 255 726 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 143 477 483 598 742 853 892 Size: 127 16 334 6 115 144 111 39 Cuts at: 892 981 Size: 89 Fragments arranged by size: 334 144 127 115 111 89 39 16 6 Bpu10I CC'TnA_GC Cuts at: 0 368 607 981 Size: 368 239 374 Fragments arranged by size: 374 368 239 BsaI GGTCTCn'nnnn_ Cuts at: 0 867 981 Size: 867 114 BsaHI Gr'CG_yC Cuts at: 0 914 953 981 Size: 914 39 28 Fragments arranged by size: 914 39 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 270 487 981 Size: 270 217 494 Fragments arranged by size: 494 270 217 BseYI C'CCAG_C Cuts at: 0 847 981 Size: 847 134 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 981 Size: 634 347 BsiEI CG_ry'CG Cuts at: 0 483 713 981 Size: 483 230 268 Fragments arranged by size: 483 268 230 BsiHKAI G_wGCw'C Cuts at: 0 336 632 981 Size: 336 296 349 Fragments arranged by size: 349 336 296 BsmBI CGTCTCn'nnnn_ Cuts at: 0 172 981 Size: 172 809 Bsp1286I G_dGCh'C Cuts at: 0 336 632 887 981 Size: 336 296 255 94 Fragments arranged by size: 336 296 255 94 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 239 651 981 Size: 239 412 330 Fragments arranged by size: 412 330 239 BsrBI CCG'CTC Cuts at: 0 594 981 Size: 594 387 BsrFI r'CCGG_y Cuts at: 0 357 949 981 Size: 357 592 32 Fragments arranged by size: 592 357 32 BssHII G'CGCG_C Cuts at: 0 541 851 981 Size: 541 310 130 Fragments arranged by size: 541 310 130 BstAPI GCAn_nnn'nTGC Cuts at: 0 140 981 Size: 140 841 BstYI r'GATC_y Cuts at: 0 488 981 Size: 488 493 BtgI C'CryG_G Cuts at: 0 190 398 840 900 981 Size: 190 208 442 60 81 Fragments arranged by size: 442 208 190 81 60 DrdI GACnn_nn'nnGTC Cuts at: 0 661 981 Size: 661 320 EaeI y'GGCC_r Cuts at: 0 389 710 947 981 Size: 389 321 237 34 Fragments arranged by size: 389 321 237 34 EagI C'GGCC_G Cuts at: 0 710 981 Size: 710 271 EarI CTCTTCn'nnn_ Cuts at: 0 150 981 Size: 150 831 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 748 981 Size: 748 233 EcoICRI GAG'CTC Cuts at: 0 630 981 Size: 630 351 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 143 477 483 598 742 853 892 Size: 127 16 334 6 115 144 111 39 Cuts at: 892 981 Size: 89 Fragments arranged by size: 334 144 127 115 111 89 39 16 6 EcoNI CCTnn'n_nnAGG Cuts at: 0 67 981 Size: 67 914 EcoO109I rG'GnC_Cy Cuts at: 0 75 185 981 Size: 75 110 796 Fragments arranged by size: 796 110 75 HaeII r_GCGC'y Cuts at: 0 757 829 917 981 Size: 757 72 88 64 Fragments arranged by size: 757 88 72 64 KasI G'GCGC_C Cuts at: 0 913 981 Size: 913 68 KpnI G_GTAC'C Cuts at: 0 678 981 Size: 678 303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 701 830 981 Size: 701 129 151 Fragments arranged by size: 701 151 129 MspA1I CmG'CkG Cuts at: 0 350 400 567 576 762 842 936 981 Size: 350 50 167 9 186 80 94 45 Fragments arranged by size: 350 186 167 94 80 50 45 9 NaeI GCC'GGC Cuts at: 0 359 951 981 Size: 359 592 30 Fragments arranged by size: 592 359 30 NarI GG'CG_CC Cuts at: 0 914 981 Size: 914 67 NgoMIV G'CCGG_C Cuts at: 0 357 949 981 Size: 357 592 32 Fragments arranged by size: 592 357 32 PflMI CCAn_nnn'nTGG Cuts at: 0 196 567 981 Size: 196 371 414 Fragments arranged by size: 414 371 196 PfoI T'CCnGG_A Cuts at: 0 159 981 Size: 159 822 PpuMI rG'GwC_Cy Cuts at: 0 75 185 981 Size: 75 110 796 Fragments arranged by size: 796 110 75 PshAI GACnn'nnGTC Cuts at: 0 712 981 Size: 712 269 PstI C_TGCA'G Cuts at: 0 236 981 Size: 236 745 PvuII CAG'CTG Cuts at: 0 567 576 981 Size: 567 9 405 Fragments arranged by size: 567 405 9 SacI G_AGCT'C Cuts at: 0 632 981 Size: 632 349 SacII CC_GC'GG Cuts at: 0 401 843 981 Size: 401 442 138 Fragments arranged by size: 442 401 138 SanDI GG'GwC_CC Cuts at: 0 75 185 981 Size: 75 110 796 Fragments arranged by size: 796 110 75 SapI GCTCTTCn'nnn_ Cuts at: 0 150 981 Size: 150 831 SexAI A'CCwGG_T Cuts at: 0 670 981 Size: 670 311 SfcI C'TryA_G Cuts at: 0 232 981 Size: 232 749 SfoI GGC'GCC Cuts at: 0 915 981 Size: 915 66 SmaI CCC'GGG Cuts at: 0 240 981 Size: 240 741 SrfI GCCC'GGGC Cuts at: 0 240 981 Size: 240 741 StyI C'CwwG_G Cuts at: 0 68 392 981 Size: 68 324 589 Fragments arranged by size: 589 324 68 XmaI C'CCGG_G Cuts at: 0 238 981 Size: 238 743 Enzymes that do cut: AarI Acc65I AfeI AlwNI ApaLI AvaI BanI BanII BbeI BcgI BclI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI BtgI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI KpnI MmeI MspA1I NaeI NarI NgoMIV PflMI PfoI PpuMI PshAI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SfoI SmaI SrfI StyI XmaI Enzymes that do not cut: AatII AccI AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI