(Linear) (Six-base) MAPSORT of: KIBB9670.seq  Check: 1626  from: 1  to: 819



KIBB9670.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AccI GT'mk_AC

Cuts at:      0     269     819 
   Size:        269     550
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     408     819 
   Size:        408     411
 
AleI CACnn'nnGTG

Cuts at:      0     217     819 
   Size:        217     602
 
BanII G_rGCy'C

Cuts at:      0       7     819 
   Size:          7     812
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     145     330     432     592     819 
   Size:        145     185     102     160     227

  Fragments arranged by size: 

                 227     185     160     145     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     224     819 
   Size:        224     595
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      84     100     493     819 
   Size:         84      16     393     326

  Fragments arranged by size: 

                 393     326      84      16
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     488     572     740     819 
   Size:        404      84      84     168      79

  Fragments arranged by size: 

                 404     168      84      84      79
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      72     819 
   Size:         72     747
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     423     591     819 
   Size:         25     398     168     228

  Fragments arranged by size: 

                 398     228     168      25
 
BsmI GAATG_Cn'

Cuts at:      0     238     819 
   Size:        238     581
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     819 
   Size:          7     812
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      54     338     819 
   Size:         54     284     481

  Fragments arranged by size: 

                 481     284      54
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     578     819 
   Size:        578     241
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     244     819 
   Size:        244     575
 
BstYI r'GATC_y

Cuts at:      0     794     819 
   Size:        794      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      29     819 
   Size:         29     790
 
BtsI GCAGTG_nn'

Cuts at:      0     579     632     819 
   Size:        579      53     187

  Fragments arranged by size: 

                 579     187      53
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     141     176     477     512     819 
   Size:        141      35     301      35     307

  Fragments arranged by size: 

                 307     301     141      35      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     525     819 
   Size:        525     294
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     404     408     488     572     740     819 
   Size:        404       4      80      84     168      79

  Fragments arranged by size: 

                 404     168      84      80      79       4
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     288     819 
   Size:        288     531
 
HincII GTy'rAC

Cuts at:      0     270     819 
   Size:        270     549
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     639     819 
   Size:        639     180
 
MslI CAynn'nnrTG

Cuts at:      0      92     217     819 
   Size:         92     125     602

  Fragments arranged by size: 

                 602     125      92
 
PstI C_TGCA'G

Cuts at:      0     639     819 
   Size:        639     180
 
SalI G'TCGA_C

Cuts at:      0     268     819 
   Size:        268     551
 
SfcI C'TryA_G

Cuts at:      0     635     681     819 
   Size:        635      46     138

  Fragments arranged by size: 

                 635     138      46
 
StuI AGG'CCT

Cuts at:      0     757     819 
   Size:        757      62
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     473     819 
   Size:        473     346
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     379     819 
   Size:        379     440
 
XmnI GAAnn'nnTTC

Cuts at:      0     337     589     819 
   Size:        337     252     230

  Fragments arranged by size: 

                 337     252     230
 

 Enzymes that do cut:

     AccI     AcuI     AleI    BanII     BbsI     BmrI     BplI     BpmI
     BsaI     BsgI     BsmI Bsp1286I    BspMI   BstAPI    BstXI    BstYI
    BtgZI     BtsI    CspCI     EarI  Eco57MI     FalI   HincII     MmeI
     MslI     PstI     SalI     SfcI     StuI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI