Restriction map for
pF1KB9667
(Linear) (Six-base) MAPSORT of: KIBB9667.seq Check: 8341 from: 1 to: 930
KIBB9667.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AclI AA'CG_TT
Cuts at: 0 819 930
Size: 819 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 930
Size: 34 896
AfeI AGC'GCT
Cuts at: 0 763 930
Size: 763 167
AleI CACnn'nnGTG
Cuts at: 0 653 930
Size: 653 277
AlwNI CAG_nnn'CTG
Cuts at: 0 12 930
Size: 12 918
ApaLI G'TGCA_C
Cuts at: 0 738 930
Size: 738 192
ApoI r'AATT_y
Cuts at: 0 414 508 930
Size: 414 94 422
Fragments arranged by size:
422 414 94
AvaI C'yCGr_G
Cuts at: 0 641 930
Size: 641 289
BanII G_rGCy'C
Cuts at: 0 63 688 930
Size: 63 625 242
Fragments arranged by size:
625 242 63
BlpI GC'TnA_GC
Cuts at: 0 74 930
Size: 74 856
Bme1580I G_kGCm'C
Cuts at: 0 742 930
Size: 742 188
BmgBI CAC'GTC
Cuts at: 0 651 930
Size: 651 279
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 930
Size: 59 871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 740 930
Size: 482 258 190
Fragments arranged by size:
482 258 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 930
Size: 442 488
BsaI GGTCTCn'nnnn_
Cuts at: 0 638 930
Size: 638 292
BsaAI yAC'GTr
Cuts at: 0 795 930
Size: 795 135
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 930
Size: 616 314
BsiHKAI G_wGCw'C
Cuts at: 0 688 742 930
Size: 688 54 188
Fragments arranged by size:
688 188 54
BsiWI C'GTAC_G
Cuts at: 0 787 930
Size: 787 143
BsmI GAATG_Cn'
Cuts at: 0 226 930
Size: 226 704
Bsp1286I G_dGCh'C
Cuts at: 0 63 688 742 930
Size: 63 625 54 188
Fragments arranged by size:
625 188 63 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 930
Size: 113 817
BstAPI GCAn_nnn'nTGC
Cuts at: 0 511 930
Size: 511 419
BstBI TT'CG_AA
Cuts at: 0 332 930
Size: 332 598
BstYI r'GATC_y
Cuts at: 0 489 930
Size: 489 441
Bsu36I CC'TnA_GG
Cuts at: 0 179 930
Size: 179 751
EarI CTCTTCn'nnn_
Cuts at: 0 51 254 930
Size: 51 203 676
Fragments arranged by size:
676 203 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 446 930
Size: 127 319 484
Fragments arranged by size:
484 319 127
EcoICRI GAG'CTC
Cuts at: 0 686 930
Size: 686 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 482 740 930
Size: 34 448 258 190
Fragments arranged by size:
448 258 190 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 259 930
Size: 259 671
EcoRI G'AATT_C
Cuts at: 0 414 930
Size: 414 516
FspI TGC'GCA
Cuts at: 0 799 930
Size: 799 131
FspAI rTGC'GCAy
Cuts at: 0 799 930
Size: 799 131
HaeII r_GCGC'y
Cuts at: 0 765 930
Size: 765 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 606 635 638 667 930
Size: 187 32 387 29 3 29 263
Fragments arranged by size:
387 263 187 32 29 29 3
HindIII A'AGCT_T
Cuts at: 0 451 930
Size: 451 479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 930
Size: 387 543
MslI CAynn'nnrTG
Cuts at: 0 653 930
Size: 653 277
NspI r_CATG'y
Cuts at: 0 898 930
Size: 898 32
PfoI T'CCnGG_A
Cuts at: 0 254 930
Size: 254 676
PmlI CAC'GTG
Cuts at: 0 795 930
Size: 795 135
PstI C_TGCA'G
Cuts at: 0 110 930
Size: 110 820
SacI G_AGCT'C
Cuts at: 0 688 930
Size: 688 242
SapI GCTCTTCn'nnn_
Cuts at: 0 51 930
Size: 51 879
SbfI CC_TGCA'GG
Cuts at: 0 110 930
Size: 110 820
SfcI C'TryA_G
Cuts at: 0 106 930
Size: 106 824
SmlI C'TyrA_G
Cuts at: 0 421 930
Size: 421 509
SphI G_CATG'C
Cuts at: 0 898 930
Size: 898 32
StuI AGG'CCT
Cuts at: 0 47 930
Size: 47 883
StyI C'CwwG_G
Cuts at: 0 281 930
Size: 281 649
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 930
Size: 40 890
TatI w'GTAC_w
Cuts at: 0 434 930
Size: 434 496
Tth111I GACn'n_nGTC
Cuts at: 0 649 930
Size: 649 281
XmnI GAAnn'nnTTC
Cuts at: 0 673 820 930
Size: 673 147 110
Fragments arranged by size:
673 147 110
Enzymes that do cut:
AclI AcuI AfeI AleI AlwNI ApaLI ApoI AvaI
BanII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI
BsaAI BseRI BsiHKAI BsiWI BsmI Bsp1286I BspMI BstAPI
BstBI BstYI Bsu36I EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI FspAI HaeII Hin4I HindIII MmeI MslI
NspI PfoI PmlI PstI SacI SapI SbfI SfcI
SmlI SphI StuI StyI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmtI BplI Bpu10I BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoO109I EcoRV FalI FseI HincII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI XbaI
XcmI XhoI XmaI ZraI