Restriction map for
pF1KB9664
(Linear) (Six-base) MAPSORT of: KIBB9664.seq Check: 9510 from: 1 to: 1374
KIBB9664.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AatII G_ACGT'C
Cuts at: 0 282 1374
Size: 282 1092
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 630 966 1050 1302 1374
Size: 215 415 336 84 252 72
Fragments arranged by size:
415 336 252 215 84 72
AfeI AGC'GCT
Cuts at: 0 991 1374
Size: 991 383
AgeI A'CCGG_T
Cuts at: 0 1094 1374
Size: 1094 280
AvaI C'yCGr_G
Cuts at: 0 741 1374
Size: 741 633
BamHI G'GATC_C
Cuts at: 0 441 1374
Size: 441 933
BanII G_rGCy'C
Cuts at: 0 742 826 910 1078 1162 1330 1374
Size: 742 84 84 168 84 168 44
Fragments arranged by size:
742 168 168 84 84 84 44
BglI GCCn_nnn'nGGC
Cuts at: 0 419 1374
Size: 419 955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1214 1298 1374
Size: 962 252 84 76
Fragments arranged by size:
962 252 84 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 810 1374
Size: 189 621 564
Fragments arranged by size:
621 564 189
BsaI GGTCTCn'nnnn_
Cuts at: 0 674 1374
Size: 674 700
BsaBI GATnn'nnATC
Cuts at: 0 480 581 1374
Size: 480 101 793
Fragments arranged by size:
793 480 101
BsaHI Gr'CG_yC
Cuts at: 0 279 1374
Size: 279 1095
BsaWI w'CCGG_w
Cuts at: 0 1094 1374
Size: 1094 280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 729 759 813 843 1374
Size: 729 30 54 30 531
Fragments arranged by size:
729 531 54 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 979 1243 1374
Size: 538 441 264 131
Fragments arranged by size:
538 441 264 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 985 1317 1374
Size: 901 84 332 57
Fragments arranged by size:
901 332 84 57
BsiHKAI G_wGCw'C
Cuts at: 0 742 826 910 1078 1162 1330 1374
Size: 742 84 84 168 84 168 44
Fragments arranged by size:
742 168 168 84 84 84 44
BsmBI CGTCTCn'nnnn_
Cuts at: 0 286 1374
Size: 286 1088
Bsp1286I G_dGCh'C
Cuts at: 0 742 826 910 1078 1162 1330 1374
Size: 742 84 84 168 84 168 44
Fragments arranged by size:
742 168 168 84 84 84 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1007 1374
Size: 1007 367
BsrDI GCAATG_nn'
Cuts at: 0 721 1374
Size: 721 653
BsrFI r'CCGG_y
Cuts at: 0 1094 1374
Size: 1094 280
BstBI TT'CG_AA
Cuts at: 0 164 1374
Size: 164 1210
BstYI r'GATC_y
Cuts at: 0 441 1164 1365 1374
Size: 441 723 201 9
Fragments arranged by size:
723 441 201 9
Bsu36I CC'TnA_GG
Cuts at: 0 257 1374
Size: 257 1117
BtsI GCAGTG_nn'
Cuts at: 0 602 1374
Size: 602 772
EaeI y'GGCC_r
Cuts at: 0 2 420 1374
Size: 2 418 954
Fragments arranged by size:
954 418 2
EarI CTCTTCn'nnn_
Cuts at: 0 152 234 1374
Size: 152 82 1140
Fragments arranged by size:
1140 152 82
EcoICRI GAG'CTC
Cuts at: 0 740 824 908 1076 1160 1328 1374
Size: 740 84 84 168 84 168 46
Fragments arranged by size:
740 168 168 84 84 84 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 630 962 966 1050 1214 1298 1302
Size: 215 415 332 4 84 164 84 4
Cuts at: 1302 1374
Size: 72
Fragments arranged by size:
415 332 215 164 84 84 72 4
4
EcoO109I rG'GnC_Cy
Cuts at: 0 1034 1374
Size: 1034 340
HaeII r_GCGC'y
Cuts at: 0 993 1374
Size: 993 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 729 761 813 845 969 1001 1149 1181
Size: 729 32 52 32 124 32 148 32
Cuts at: 1181 1374
Size: 193
Fragments arranged by size:
729 193 148 124 52 32 32 32
32
HincII GTy'rAC
Cuts at: 0 91 1374
Size: 91 1283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1355 1374
Size: 1355 19
MscI TGG'CCA
Cuts at: 0 4 422 1374
Size: 4 418 952
Fragments arranged by size:
952 418 4
MslI CAynn'nnrTG
Cuts at: 0 587 773 1374
Size: 587 186 601
Fragments arranged by size:
601 587 186
MspA1I CmG'CkG
Cuts at: 0 1350 1374
Size: 1350 24
NdeI CA'TA_TG
Cuts at: 0 588 1374
Size: 588 786
PsiI TTA'TAA
Cuts at: 0 788 872 1374
Size: 788 84 502
Fragments arranged by size:
788 502 84
PspXI vC'TCGA_Gb
Cuts at: 0 741 1374
Size: 741 633
PstI C_TGCA'G
Cuts at: 0 609 1374
Size: 609 765
SacI G_AGCT'C
Cuts at: 0 742 826 910 1078 1162 1330 1374
Size: 742 84 84 168 84 168 44
Fragments arranged by size:
742 168 168 84 84 84 44
SfcI C'TryA_G
Cuts at: 0 605 1374
Size: 605 769
SgrAI Cr'CCGG_yG
Cuts at: 0 1094 1374
Size: 1094 280
SmlI C'TyrA_G
Cuts at: 0 204 741 825 1374
Size: 204 537 84 549
Fragments arranged by size:
549 537 204 84
TatI w'GTAC_w
Cuts at: 0 7 1017 1269 1374
Size: 7 1010 252 105
Fragments arranged by size:
1010 252 105 7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 547 1374
Size: 547 827
XhoI C'TCGA_G
Cuts at: 0 741 1374
Size: 741 633
XmnI GAAnn'nnTTC
Cuts at: 0 727 811 1063 1147 1315 1374
Size: 727 84 252 84 168 59
Fragments arranged by size:
727 252 168 84 84 59
ZraI GAC'GTC
Cuts at: 0 280 1374
Size: 280 1094
Enzymes that do cut:
AatII AcuI AfeI AgeI AvaI BamHI BanII BglI
BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstBI
BstYI Bsu36I BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HincII MmeI MscI MslI MspA1I NdeI
PsiI PspXI PstI SacI SfcI SgrAI SmlI TatI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BseYI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TstI Tth111I XbaI XmaI