Restriction map for
pF1KB9663
(Linear) (Six-base) MAPSORT of: KIBB9663.seq Check: 4753 from: 1 to: 672
KIBB9663.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AccI GT'mk_AC
Cuts at: 0 132 672
Size: 132 540
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 672
Size: 111 561
ApoI r'AATT_y
Cuts at: 0 392 672
Size: 392 280
AvaI C'yCGr_G
Cuts at: 0 590 672
Size: 590 82
BamHI G'GATC_C
Cuts at: 0 308 672
Size: 308 364
BanII G_rGCy'C
Cuts at: 0 40 370 454 538 672
Size: 40 330 84 84 134
Fragments arranged by size:
330 134 84 84 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 619 672
Size: 619 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 494 672
Size: 494 178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 221 672
Size: 221 451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 672
Size: 422 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 105 672
Size: 24 81 567
Fragments arranged by size:
567 81 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 670 672
Size: 670 2
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 672
Size: 525 147
BsiHKAI G_wGCw'C
Cuts at: 0 40 370 454 538 672
Size: 40 330 84 84 134
Fragments arranged by size:
330 134 84 84 40
Bsp1286I G_dGCh'C
Cuts at: 0 40 370 454 538 672
Size: 40 330 84 84 134
Fragments arranged by size:
330 134 84 84 40
BsrFI r'CCGG_y
Cuts at: 0 80 434 672
Size: 80 354 238
Fragments arranged by size:
354 238 80
BstYI r'GATC_y
Cuts at: 0 224 308 672
Size: 224 84 364
Fragments arranged by size:
364 224 84
BtsI GCAGTG_nn'
Cuts at: 0 513 672
Size: 513 159
EaeI y'GGCC_r
Cuts at: 0 432 672
Size: 432 240
EarI CTCTTCn'nnn_
Cuts at: 0 566 672
Size: 566 106
EcoICRI GAG'CTC
Cuts at: 0 38 368 452 536 672
Size: 38 330 84 84 136
Fragments arranged by size:
330 136 84 84 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 422 672
Size: 111 311 250
Fragments arranged by size:
311 250 111
EcoRI G'AATT_C
Cuts at: 0 392 672
Size: 392 280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 636 672
Size: 636 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 273 305 357 389 525 557
Size: 177 32 64 32 52 32 136 32
Cuts at: 557 672
Size: 115
Fragments arranged by size:
177 136 115 64 52 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 123 144 354 672
Size: 123 21 210 318
Fragments arranged by size:
318 210 123 21
MslI CAynn'nnrTG
Cuts at: 0 251 559 672
Size: 251 308 113
Fragments arranged by size:
308 251 113
MspA1I CmG'CkG
Cuts at: 0 87 672
Size: 87 585
NaeI GCC'GGC
Cuts at: 0 82 672
Size: 82 590
NgoMIV G'CCGG_C
Cuts at: 0 80 672
Size: 80 592
SacI G_AGCT'C
Cuts at: 0 40 370 454 538 672
Size: 40 330 84 84 134
Fragments arranged by size:
330 134 84 84 40
SmaI CCC'GGG
Cuts at: 0 592 672
Size: 592 80
SmlI C'TyrA_G
Cuts at: 0 39 120 672
Size: 39 81 552
Fragments arranged by size:
552 81 39
StuI AGG'CCT
Cuts at: 0 55 672
Size: 55 617
StyI C'CwwG_G
Cuts at: 0 45 672
Size: 45 627
TatI w'GTAC_w
Cuts at: 0 169 672
Size: 169 503
XmaI C'CCGG_G
Cuts at: 0 590 672
Size: 590 82
XmnI GAAnn'nnTTC
Cuts at: 0 271 672
Size: 271 401
Enzymes that do cut:
AccI AcuI ApoI AvaI BamHI BanII BbsI BmrI
BplI BpmI BpuEI BsaI BsgI BsiHKAI Bsp1286I BsrFI
BstYI BtsI EaeI EarI EcoICRI Eco57MI EcoRI FalI
Hin4I HindIII MslI MspA1I NaeI NgoMIV SacI SmaI
SmlI StuI StyI TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI ZraI