Restriction map for
pF1KB9662
(Linear) (Six-base) MAPSORT of: KIBB9662.seq Check: 2281 from: 1 to: 1011
KIBB9662.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
Acc65I G'GTAC_C
Cuts at: 0 464 1011
Size: 464 547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 925 1011
Size: 490 435 86
Fragments arranged by size:
490 435 86
AflIII A'CryG_T
Cuts at: 0 482 1011
Size: 482 529
ApaI G_GGCC'C
Cuts at: 0 526 1011
Size: 526 485
ApoI r'AATT_y
Cuts at: 0 634 1011
Size: 634 377
AvaI C'yCGr_G
Cuts at: 0 121 197 504 664 1011
Size: 121 76 307 160 347
Fragments arranged by size:
347 307 160 121 76
BanI G'GyrC_C
Cuts at: 0 186 255 464 1011
Size: 186 69 209 547
Fragments arranged by size:
547 209 186 69
BanII G_rGCy'C
Cuts at: 0 526 1011
Size: 526 485
BbeI G_GCGC'C
Cuts at: 0 259 1011
Size: 259 752
BbsI GAAGACnn'nnnn_
Cuts at: 0 986 1011
Size: 986 25
BglI GCCn_nnn'nGGC
Cuts at: 0 309 1011
Size: 309 702
BlpI GC'TnA_GC
Cuts at: 0 170 1011
Size: 170 841
Bme1580I G_kGCm'C
Cuts at: 0 526 1011
Size: 526 485
BmgBI CAC'GTC
Cuts at: 0 273 485 1011
Size: 273 212 526
Fragments arranged by size:
526 273 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 790 1011
Size: 790 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 862 1011
Size: 2 860 149
Fragments arranged by size:
860 149 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 1011
Size: 367 644
BsaAI yAC'GTr
Cuts at: 0 532 1011
Size: 532 479
BsaHI Gr'CG_yC
Cuts at: 0 256 1011
Size: 256 755
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 489 492 1011
Size: 262 227 3 519
Fragments arranged by size:
519 262 227 3
BseYI C'CCAG_C
Cuts at: 0 563 1011
Size: 563 448
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 831 1011
Size: 408 423 180
Fragments arranged by size:
423 408 180
BsiEI CG_ry'CG
Cuts at: 0 32 648 1011
Size: 32 616 363
Fragments arranged by size:
616 363 32
BsiHKAI G_wGCw'C
Cuts at: 0 128 365 428 848 1011
Size: 128 237 63 420 163
Fragments arranged by size:
420 237 163 128 63
Bsp1286I G_dGCh'C
Cuts at: 0 128 365 428 526 848 1011
Size: 128 237 63 98 322 163
Fragments arranged by size:
322 237 163 128 98 63
BsrBI CCG'CTC
Cuts at: 0 293 1011
Size: 293 718
BsrFI r'CCGG_y
Cuts at: 0 889 1011
Size: 889 122
BsrGI T'GTAC_A
Cuts at: 0 278 1011
Size: 278 733
BssHII G'CGCG_C
Cuts at: 0 549 836 1011
Size: 549 287 175
Fragments arranged by size:
549 287 175
Bsu36I CC'TnA_GG
Cuts at: 0 460 1011
Size: 460 551
BtgI C'CryG_G
Cuts at: 0 178 488 1004 1011
Size: 178 310 516 7
Fragments arranged by size:
516 310 178 7
BtsI GCAGTG_nn'
Cuts at: 0 409 1011
Size: 409 602
DraIII CAC_nnn'GTG
Cuts at: 0 809 1011
Size: 809 202
EaeI y'GGCC_r
Cuts at: 0 29 1011
Size: 29 982
EagI C'GGCC_G
Cuts at: 0 29 1011
Size: 29 982
EarI CTCTTCn'nnn_
Cuts at: 0 67 686 740 1011
Size: 67 619 54 271
Fragments arranged by size:
619 271 67 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 490 862 925 1011
Size: 2 488 372 63 86
Fragments arranged by size:
488 372 86 63 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 17 1011
Size: 17 994
EcoO109I rG'GnC_Cy
Cuts at: 0 194 201 522 523 1011
Size: 194 7 321 1 488
Fragments arranged by size:
488 321 194 7 1
HaeII r_GCGC'y
Cuts at: 0 10 108 259 1011
Size: 10 98 151 752
Fragments arranged by size:
752 151 98 10
HindIII A'AGCT_T
Cuts at: 0 937 1011
Size: 937 74
KasI G'GCGC_C
Cuts at: 0 255 1011
Size: 255 756
KpnI G_GTAC'C
Cuts at: 0 468 1011
Size: 468 543
MslI CAynn'nnrTG
Cuts at: 0 487 762 1011
Size: 487 275 249
Fragments arranged by size:
487 275 249
MspA1I CmG'CkG
Cuts at: 0 146 180 212 334 396 629 1011
Size: 146 34 32 122 62 233 382
Fragments arranged by size:
382 233 146 122 62 34 32
NarI GG'CG_CC
Cuts at: 0 256 1011
Size: 256 755
NcoI C'CATG_G
Cuts at: 0 488 1004 1011
Size: 488 516 7
Fragments arranged by size:
516 488 7
NdeI CA'TA_TG
Cuts at: 0 772 1011
Size: 772 239
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 1011
Size: 732 279
PfoI T'CCnGG_A
Cuts at: 0 18 401 1011
Size: 18 383 610
Fragments arranged by size:
610 383 18
PspOMI G'GGCC_C
Cuts at: 0 522 1011
Size: 522 489
PstI C_TGCA'G
Cuts at: 0 93 951 1011
Size: 93 858 60
Fragments arranged by size:
858 93 60
PvuII CAG'CTG
Cuts at: 0 212 629 1011
Size: 212 417 382
Fragments arranged by size:
417 382 212
SacII CC_GC'GG
Cuts at: 0 181 1011
Size: 181 830
SexAI A'CCwGG_T
Cuts at: 0 44 1011
Size: 44 967
SfcI C'TryA_G
Cuts at: 0 89 947 1011
Size: 89 858 64
Fragments arranged by size:
858 89 64
SfoI GGC'GCC
Cuts at: 0 257 1011
Size: 257 754
SmaI CCC'GGG
Cuts at: 0 199 1011
Size: 199 812
SmlI C'TyrA_G
Cuts at: 0 382 1011
Size: 382 629
StuI AGG'CCT
Cuts at: 0 95 784 1011
Size: 95 689 227
Fragments arranged by size:
689 227 95
StyI C'CwwG_G
Cuts at: 0 476 488 1004 1011
Size: 476 12 516 7
Fragments arranged by size:
516 476 12 7
TatI w'GTAC_w
Cuts at: 0 278 1011
Size: 278 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 634 666 1011
Size: 634 32 345
Fragments arranged by size:
634 345 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 918 1011
Size: 918 93
XmaI C'CCGG_G
Cuts at: 0 197 1011
Size: 197 814
Enzymes that do cut:
Acc65I AcuI AflIII ApaI ApoI AvaI BanI BanII
BbeI BbsI BglI BlpI Bme1580I BmgBI BmrI BpmI
BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BsrFI BsrGI BssHII Bsu36I BtgI BtsI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I HaeII
HindIII KasI KpnI MslI MspA1I NarI NcoI NdeI
PflMI PfoI PspOMI PstI PvuII SacII SexAI SfcI
SfoI SmaI SmlI StuI StyI TatI TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BmtI BplI Bpu10I BsaI BsaBI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HpaI MfeI MluI
MmeI MscI NaeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XhoI XmnI ZraI