Restriction map for
pF1KB9661
(Linear) (Six-base) MAPSORT of: KIBB9661.seq Check: 6806 from: 1 to: 1029
KIBB9661.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 584 1029
Size: 584 445
ApaI G_GGCC'C
Cuts at: 0 544 1029
Size: 544 485
ApaLI G'TGCA_C
Cuts at: 0 42 854 1029
Size: 42 812 175
Fragments arranged by size:
812 175 42
ApoI r'AATT_y
Cuts at: 0 652 1029
Size: 652 377
AvaI C'yCGr_G
Cuts at: 0 277 556 1029
Size: 277 279 473
Fragments arranged by size:
473 279 277
BanII G_rGCy'C
Cuts at: 0 119 278 544 1029
Size: 119 159 266 485
Fragments arranged by size:
485 266 159 119
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 909 1004 1029
Size: 211 698 95 25
Fragments arranged by size:
698 211 95 25
BbvCI CC'TCA_GC
Cuts at: 0 430 1029
Size: 430 599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 835 869 994 1028 1029
Size: 835 34 125 34 1
Fragments arranged by size:
835 125 34 34 1
BciVI GTATCCnnnnn_n'
Cuts at: 0 492 1029
Size: 492 537
BglII A'GATC_T
Cuts at: 0 452 1029
Size: 452 577
Bme1580I G_kGCm'C
Cuts at: 0 46 544 858 1029
Size: 46 498 314 171
Fragments arranged by size:
498 314 171 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 808 1029
Size: 808 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1029
Size: 2 1027
Bpu10I CC'TnA_GC
Cuts at: 0 430 1029
Size: 430 599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 1029
Size: 267 762
BsaAI yAC'GTr
Cuts at: 0 568 1029
Size: 568 461
BsaHI Gr'CG_yC
Cuts at: 0 914 1029
Size: 914 115
BsaWI w'CCGG_w
Cuts at: 0 165 1029
Size: 165 864
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 1029
Size: 439 590
BseYI C'CCAG_C
Cuts at: 0 581 1029
Size: 581 448
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 849 1029
Size: 135 714 180
Fragments arranged by size:
714 180 135
BsiEI CG_ry'CG
Cuts at: 0 166 1029
Size: 166 863
BsiHKAI G_wGCw'C
Cuts at: 0 46 858 1029
Size: 46 812 171
Fragments arranged by size:
812 171 46
Bsp1286I G_dGCh'C
Cuts at: 0 46 119 278 544 858 1029
Size: 46 73 159 266 314 171
Fragments arranged by size:
314 266 171 159 73 46
BsrDI GCAATG_nn'
Cuts at: 0 519 1029
Size: 519 510
BstEII G'GTnAC_C
Cuts at: 0 180 1029
Size: 180 849
BstYI r'GATC_y
Cuts at: 0 416 452 1029
Size: 416 36 577
Fragments arranged by size:
577 416 36
BtgI C'CryG_G
Cuts at: 0 323 1029
Size: 323 706
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 398 773 1029
Size: 398 375 256
Fragments arranged by size:
398 375 256
BtsI GCAGTG_nn'
Cuts at: 0 856 1029
Size: 856 173
DraIII CAC_nnn'GTG
Cuts at: 0 827 1029
Size: 827 202
EarI CTCTTCn'nnn_
Cuts at: 0 251 257 525 1029
Size: 251 6 268 504
Fragments arranged by size:
504 268 251 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 84 1029
Size: 84 945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1029
Size: 2 1027
EcoO109I rG'GnC_Cy
Cuts at: 0 540 541 1029
Size: 540 1 488
Fragments arranged by size:
540 488 1
HaeII r_GCGC'y
Cuts at: 0 99 556 1029
Size: 99 457 473
Fragments arranged by size:
473 457 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 174 206 1029
Size: 174 32 823
Fragments arranged by size:
823 174 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1029
Size: 1010 19
MslI CAynn'nnrTG
Cuts at: 0 566 780 1029
Size: 566 214 249
Fragments arranged by size:
566 249 214
MspA1I CmG'CkG
Cuts at: 0 352 647 1029
Size: 352 295 382
Fragments arranged by size:
382 352 295
PfoI T'CCnGG_A
Cuts at: 0 18 419 523 1029
Size: 18 401 104 506
Fragments arranged by size:
506 401 104 18
PshAI GACnn'nnGTC
Cuts at: 0 352 1029
Size: 352 677
PsiI TTA'TAA
Cuts at: 0 299 1029
Size: 299 730
PspOMI G'GGCC_C
Cuts at: 0 540 1029
Size: 540 489
PstI C_TGCA'G
Cuts at: 0 863 1029
Size: 863 166
PvuII CAG'CTG
Cuts at: 0 352 647 1029
Size: 352 295 382
Fragments arranged by size:
382 352 295
SfcI C'TryA_G
Cuts at: 0 859 965 1029
Size: 859 106 64
Fragments arranged by size:
859 106 64
SmlI C'TyrA_G
Cuts at: 0 246 1029
Size: 246 783
StyI C'CwwG_G
Cuts at: 0 494 1029
Size: 494 535
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 674 1029
Size: 674 355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 25 46 57 1029
Size: 14 11 21 11 972
Fragments arranged by size:
972 21 14 11 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 907 1029
Size: 907 122
Enzymes that do cut:
AlfI ApaI ApaLI ApoI AvaI BanII BbsI BbvCI
BcgI BciVI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrDI BstEII BstYI BtgI BtgZI BtsI DraIII
EarI EciI Eco57MI EcoO109I HaeII Hin4I MmeI MslI
MspA1I PfoI PshAI PsiI PspOMI PstI PvuII SfcI
SmlI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BsaI BsaBI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EaeI EagI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI