Restriction map for
pF1KB9658
(Linear) (Six-base) MAPSORT of: KIBB9658.seq Check: 9015 from: 1 to: 969
KIBB9658.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 689 816 969
Size: 689 127 153
Fragments arranged by size:
689 153 127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 736 757 900 969
Size: 201 535 21 143 69
Fragments arranged by size:
535 201 143 69 21
AfeI AGC'GCT
Cuts at: 0 329 969
Size: 329 640
AflII C'TTAA_G
Cuts at: 0 838 969
Size: 838 131
AflIII A'CryG_T
Cuts at: 0 428 774 969
Size: 428 346 195
Fragments arranged by size:
428 346 195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 969
Size: 160 809
AlwNI CAG_nnn'CTG
Cuts at: 0 134 360 969
Size: 134 226 609
Fragments arranged by size:
609 226 134
ApaI G_GGCC'C
Cuts at: 0 469 569 969
Size: 469 100 400
Fragments arranged by size:
469 400 100
AvaI C'yCGr_G
Cuts at: 0 244 289 310 435 562 568 955 969
Size: 244 45 21 125 127 6 387 14
Fragments arranged by size:
387 244 127 125 45 21 14 6
BanI G'GyrC_C
Cuts at: 0 439 969
Size: 439 530
BanII G_rGCy'C
Cuts at: 0 469 569 931 969
Size: 469 100 362 38
Fragments arranged by size:
469 362 100 38
BbeI G_GCGC'C
Cuts at: 0 443 969
Size: 443 526
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 814 969
Size: 241 573 155
Fragments arranged by size:
573 241 155
BglI GCCn_nnn'nGGC
Cuts at: 0 108 448 969
Size: 108 340 521
Fragments arranged by size:
521 340 108
BlpI GC'TnA_GC
Cuts at: 0 13 178 969
Size: 13 165 791
Fragments arranged by size:
791 165 13
Bme1580I G_kGCm'C
Cuts at: 0 203 469 569 969
Size: 203 266 100 400
Fragments arranged by size:
400 266 203 100
BmgBI CAC'GTC
Cuts at: 0 90 969
Size: 90 879
BmrI ACTGGGnnnn_n'
Cuts at: 0 115 340 750 969
Size: 115 225 410 219
Fragments arranged by size:
410 225 219 115
BmtI G_CTAG'C
Cuts at: 0 533 969
Size: 533 436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 945 969
Size: 945 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 969
Size: 817 152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 969
Size: 64 905
BsaHI Gr'CG_yC
Cuts at: 0 307 440 875 969
Size: 307 133 435 94
Fragments arranged by size:
435 307 133 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 616 646 931 961 969
Size: 616 30 285 30 8
Fragments arranged by size:
616 285 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 939 969
Size: 939 30
BseYI C'CCAG_C
Cuts at: 0 211 462 969
Size: 211 251 507
Fragments arranged by size:
507 251 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 969
Size: 120 849
BsiEI CG_ry'CG
Cuts at: 0 227 969
Size: 227 742
BsmBI CGTCTCn'nnnn_
Cuts at: 0 866 969
Size: 866 103
Bsp1286I G_dGCh'C
Cuts at: 0 203 469 569 931 969
Size: 203 266 100 362 38
Fragments arranged by size:
362 266 203 100 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 689 755 816 969
Size: 83 606 66 61 153
Fragments arranged by size:
606 153 83 66 61
BsrBI CCG'CTC
Cuts at: 0 177 969
Size: 177 792
BsrFI r'CCGG_y
Cuts at: 0 154 442 969
Size: 154 288 527
Fragments arranged by size:
527 288 154
BssHII G'CGCG_C
Cuts at: 0 111 969
Size: 111 858
BssSI C'ACGA_G
Cuts at: 0 646 969
Size: 646 323
BstAPI GCAn_nnn'nTGC
Cuts at: 0 134 348 546 969
Size: 134 214 198 423
Fragments arranged by size:
423 214 198 134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 969
Size: 45 924
BstYI r'GATC_y
Cuts at: 0 249 969
Size: 249 720
BtgI C'CryG_G
Cuts at: 0 5 50 969
Size: 5 45 919
Fragments arranged by size:
919 45 5
DraIII CAC_nnn'GTG
Cuts at: 0 188 348 969
Size: 188 160 621
Fragments arranged by size:
621 188 160
EaeI y'GGCC_r
Cuts at: 0 2 47 969
Size: 2 45 922
Fragments arranged by size:
922 45 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 700 744 969
Size: 700 44 225
Fragments arranged by size:
700 225 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 736 757 817 900 969
Size: 201 535 21 60 83 69
Fragments arranged by size:
535 201 83 69 60 21
EcoO109I rG'GnC_Cy
Cuts at: 0 131 969
Size: 131 838
HaeII r_GCGC'y
Cuts at: 0 298 331 389 443 527 969
Size: 298 33 58 54 84 442
Fragments arranged by size:
442 298 84 58 54 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 931 963 969
Size: 616 32 283 32 6
Fragments arranged by size:
616 283 32 32 6
HincII GTy'rAC
Cuts at: 0 63 969
Size: 63 906
HindIII A'AGCT_T
Cuts at: 0 366 969
Size: 366 603
KasI G'GCGC_C
Cuts at: 0 439 969
Size: 439 530
MluI A'CGCG_T
Cuts at: 0 428 774 969
Size: 428 346 195
Fragments arranged by size:
428 346 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 969
Size: 659 310
MscI TGG'CCA
Cuts at: 0 49 969
Size: 49 920
MslI CAynn'nnrTG
Cuts at: 0 658 969
Size: 658 311
MspA1I CmG'CkG
Cuts at: 0 7 45 360 546 969
Size: 7 38 315 186 423
Fragments arranged by size:
423 315 186 38 7
NaeI GCC'GGC
Cuts at: 0 156 444 969
Size: 156 288 525
Fragments arranged by size:
525 288 156
NarI GG'CG_CC
Cuts at: 0 440 969
Size: 440 529
NcoI C'CATG_G
Cuts at: 0 50 969
Size: 50 919
NgoMIV G'CCGG_C
Cuts at: 0 154 442 969
Size: 154 288 527
Fragments arranged by size:
527 288 154
NheI G'CTAG_C
Cuts at: 0 529 969
Size: 529 440
PspOMI G'GGCC_C
Cuts at: 0 465 565 969
Size: 465 100 404
Fragments arranged by size:
465 404 100
PspXI vC'TCGA_Gb
Cuts at: 0 244 969
Size: 244 725
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 733 765 969
Size: 733 32 204
Fragments arranged by size:
733 204 32
PstI C_TGCA'G
Cuts at: 0 149 969
Size: 149 820
PvuII CAG'CTG
Cuts at: 0 546 969
Size: 546 423
SacII CC_GC'GG
Cuts at: 0 8 969
Size: 8 961
SfcI C'TryA_G
Cuts at: 0 145 969
Size: 145 824
SfoI GGC'GCC
Cuts at: 0 441 969
Size: 441 528
SgrAI Cr'CCGG_yG
Cuts at: 0 442 969
Size: 442 527
SmaI CCC'GGG
Cuts at: 0 437 564 570 969
Size: 437 127 6 399
Fragments arranged by size:
437 399 127 6
SmlI C'TyrA_G
Cuts at: 0 79 244 838 969
Size: 79 165 594 131
Fragments arranged by size:
594 165 131 79
SrfI GCCC'GGGC
Cuts at: 0 570 969
Size: 570 399
StyI C'CwwG_G
Cuts at: 0 50 695 969
Size: 50 645 274
Fragments arranged by size:
645 274 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 359 969
Size: 241 118 610
Fragments arranged by size:
610 241 118
TatI w'GTAC_w
Cuts at: 0 254 969
Size: 254 715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 969
Size: 42 927
XhoI C'TCGA_G
Cuts at: 0 244 969
Size: 244 725
XmaI C'CCGG_G
Cuts at: 0 435 562 568 969
Size: 435 127 6 401
Fragments arranged by size:
435 401 127 6
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AlfI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI DraIII
EaeI EciI Eco57MI EcoO109I HaeII Hin4I HincII HindIII
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI PspOMI PspXI PsrI PstI PvuII
SacII SfcI SfoI SgrAI SmaI SmlI SrfI StyI
TaqII TatI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII Bpu10I BsaI
BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI
MfeI NdeI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SnaBI SpeI SphI SspI
StuI SwaI TstI Tth111I XbaI XmnI ZraI