Restriction map for
pF1KB9657
(Linear) (Six-base) MAPSORT of: KIBB9657.seq Check: 1457 from: 1 to: 606
KIBB9657.orf
With 182 enzymes: SgfI *
May 10, 2010 11:26 ..
AflIII A'CryG_T
Cuts at: 0 11 606
Size: 11 595
AlwNI CAG_nnn'CTG
Cuts at: 0 369 606
Size: 369 237
ApaI G_GGCC'C
Cuts at: 0 155 606
Size: 155 451
BanII G_rGCy'C
Cuts at: 0 155 606
Size: 155 451
BglI GCCn_nnn'nGGC
Cuts at: 0 75 606
Size: 75 531
BlpI GC'TnA_GC
Cuts at: 0 427 547 606
Size: 427 120 59
Fragments arranged by size:
427 120 59
Bme1580I G_kGCm'C
Cuts at: 0 155 606
Size: 155 451
BmgBI CAC'GTC
Cuts at: 0 12 606
Size: 12 594
BmrI ACTGGGnnnn_n'
Cuts at: 0 357 606
Size: 357 249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 522 606
Size: 522 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 588 606
Size: 472 116 18
Fragments arranged by size:
472 116 18
Bpu10I CC'TnA_GC
Cuts at: 0 49 606
Size: 49 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 606
Size: 430 176
BsaHI Gr'CG_yC
Cuts at: 0 176 606
Size: 176 430
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 606
Size: 325 281
BsiEI CG_ry'CG
Cuts at: 0 38 606
Size: 38 568
Bsp1286I G_dGCh'C
Cuts at: 0 155 606
Size: 155 451
BsrBI CCG'CTC
Cuts at: 0 92 543 606
Size: 92 451 63
Fragments arranged by size:
451 92 63
BsrFI r'CCGG_y
Cuts at: 0 33 74 606
Size: 33 41 532
Fragments arranged by size:
532 41 33
BssSI C'ACGA_G
Cuts at: 0 538 606
Size: 538 68
BtgI C'CryG_G
Cuts at: 0 164 606
Size: 164 442
EaeI y'GGCC_r
Cuts at: 0 35 335 455 606
Size: 35 300 120 151
Fragments arranged by size:
300 151 120 35
EagI C'GGCC_G
Cuts at: 0 35 606
Size: 35 571
EarI CTCTTCn'nnn_
Cuts at: 0 59 606
Size: 59 547
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 173 606
Size: 173 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 588 606
Size: 472 116 18
Fragments arranged by size:
472 116 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 478 606
Size: 478 128
EcoO109I rG'GnC_Cy
Cuts at: 0 151 483 581 606
Size: 151 332 98 25
Fragments arranged by size:
332 151 98 25
HaeII r_GCGC'y
Cuts at: 0 354 606
Size: 354 252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 606
Size: 159 447
MscI TGG'CCA
Cuts at: 0 337 606
Size: 337 269
MspA1I CmG'CkG
Cuts at: 0 166 284 327 606
Size: 166 118 43 279
Fragments arranged by size:
279 166 118 43
NaeI GCC'GGC
Cuts at: 0 35 606
Size: 35 571
NgoMIV G'CCGG_C
Cuts at: 0 33 606
Size: 33 573
PasI CC'CwG_GG
Cuts at: 0 210 606
Size: 210 396
PpuMI rG'GwC_Cy
Cuts at: 0 483 606
Size: 483 123
PspOMI G'GGCC_C
Cuts at: 0 151 606
Size: 151 455
PstI C_TGCA'G
Cuts at: 0 320 526 606
Size: 320 206 80
Fragments arranged by size:
320 206 80
SacII CC_GC'GG
Cuts at: 0 167 606
Size: 167 439
SapI GCTCTTCn'nnn_
Cuts at: 0 59 606
Size: 59 547
SexAI A'CCwGG_T
Cuts at: 0 479 606
Size: 479 127
SfcI C'TryA_G
Cuts at: 0 316 522 606
Size: 316 206 84
Fragments arranged by size:
316 206 84
SmlI C'TyrA_G
Cuts at: 0 445 606
Size: 445 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 376 552 606
Size: 376 176 54
Fragments arranged by size:
376 176 54
Enzymes that do cut:
AflIII AlwNI ApaI BanII BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaHI BseRI BsiEI
Bsp1286I BsrBI BsrFI BssSI BtgI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I HaeII MmeI MscI MspA1I
NaeI NgoMIV PasI PpuMI PspOMI PstI SacII SapI
SexAI SfcI SmlI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI