Restriction map for
pF1KB9656
(Linear) (Six-base) MAPSORT of: KIBB9656.seq Check: 656 from: 1 to: 477
KIBB9656.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 124 156 477
Size: 124 32 321
Fragments arranged by size:
321 124 32
ApoI r'AATT_y
Cuts at: 0 360 477
Size: 360 117
AvaI C'yCGr_G
Cuts at: 0 100 477
Size: 100 377
BanI G'GyrC_C
Cuts at: 0 242 477
Size: 242 235
BanII G_rGCy'C
Cuts at: 0 158 170 477
Size: 158 12 307
Fragments arranged by size:
307 158 12
BbeI G_GCGC'C
Cuts at: 0 246 477
Size: 246 231
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 477
Size: 104 373
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 144 477
Size: 144 333
Bpu10I CC'TnA_GC
Cuts at: 0 164 477
Size: 164 313
BsaHI Gr'CG_yC
Cuts at: 0 243 477
Size: 243 234
BsaWI w'CCGG_w
Cuts at: 0 69 477
Size: 69 408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 124 139 154 477
Size: 109 15 15 15 323
Fragments arranged by size:
323 109 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 118 133 148 151 166 175 208
Size: 73 45 15 15 3 15 9 33
Cuts at: 208 388 477
Size: 180 89
Fragments arranged by size:
180 89 73 45 33 15 15 15
9 3
BsiHKAI G_wGCw'C
Cuts at: 0 80 158 170 477
Size: 80 78 12 307
Fragments arranged by size:
307 80 78 12
Bsp1286I G_dGCh'C
Cuts at: 0 80 158 170 477
Size: 80 78 12 307
Fragments arranged by size:
307 80 78 12
BsrDI GCAATG_nn'
Cuts at: 0 375 477
Size: 375 102
BssHII G'CGCG_C
Cuts at: 0 41 477
Size: 41 436
Bsu36I CC'TnA_GG
Cuts at: 0 149 477
Size: 149 328
BtgI C'CryG_G
Cuts at: 0 251 477
Size: 251 226
DraI TTT'AAA
Cuts at: 0 306 477
Size: 306 171
EarI CTCTTCn'nnn_
Cuts at: 0 23 398 477
Size: 23 375 79
Fragments arranged by size:
375 79 23
EcoICRI GAG'CTC
Cuts at: 0 156 168 477
Size: 156 12 309
Fragments arranged by size:
309 156 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 235 477
Size: 190 45 242
Fragments arranged by size:
242 190 45
EcoO109I rG'GnC_Cy
Cuts at: 0 228 477
Size: 228 249
HaeII r_GCGC'y
Cuts at: 0 201 246 477
Size: 201 45 231
Fragments arranged by size:
231 201 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 109 126 141 477
Size: 94 15 17 15 336
Fragments arranged by size:
336 94 17 15 15
HindIII A'AGCT_T
Cuts at: 0 286 300 477
Size: 286 14 177
Fragments arranged by size:
286 177 14
KasI G'GCGC_C
Cuts at: 0 242 477
Size: 242 235
NarI GG'CG_CC
Cuts at: 0 243 477
Size: 243 234
NcoI C'CATG_G
Cuts at: 0 251 477
Size: 251 226
PfoI T'CCnGG_A
Cuts at: 0 220 477
Size: 220 257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 477
Size: 124 32 321
Fragments arranged by size:
321 124 32
PpuMI rG'GwC_Cy
Cuts at: 0 228 477
Size: 228 249
SacI G_AGCT'C
Cuts at: 0 158 170 477
Size: 158 12 307
Fragments arranged by size:
307 158 12
SapI GCTCTTCn'nnn_
Cuts at: 0 23 477
Size: 23 454
SfoI GGC'GCC
Cuts at: 0 244 477
Size: 244 233
StuI AGG'CCT
Cuts at: 0 66 477
Size: 66 411
StyI C'CwwG_G
Cuts at: 0 251 477
Size: 251 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 477
Size: 67 410
Enzymes that do cut:
AloI ApoI AvaI BanI BanII BbeI BbsI BplI
Bpu10I BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BsrDI
BssHII Bsu36I BtgI DraI EarI EcoICRI EcoNI EcoO109I
HaeII Hin4I HindIII KasI NarI NcoI PfoI PpiI
PpuMI SacI SapI SfoI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EciI Eco57MI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI