Restriction map for
pF1KB9651
(Linear) (Six-base) MAPSORT of: KIBB9651.seq Check: 1054 from: 1 to: 720
KIBB9651.orf
With 182 enzymes: SgfI *
March 3, 2008 11:11 ..
AflIII A'CryG_T
Cuts at: 0 497 720
Size: 497 223
AvaI C'yCGr_G
Cuts at: 0 56 364 374 435 720
Size: 56 308 10 61 285
Fragments arranged by size:
308 285 61 56 10
AvrII C'CTAG_G
Cuts at: 0 123 720
Size: 123 597
BanI G'GyrC_C
Cuts at: 0 127 399 523 597 720
Size: 127 272 124 74 123
Fragments arranged by size:
272 127 124 123 74
BbeI G_GCGC'C
Cuts at: 0 403 527 720
Size: 403 124 193
Fragments arranged by size:
403 193 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 270 720
Size: 270 450
BbvCI CC'TCA_GC
Cuts at: 0 538 720
Size: 538 182
Bme1580I G_kGCm'C
Cuts at: 0 467 602 720
Size: 467 135 118
Fragments arranged by size:
467 135 118
Bpu10I CC'TnA_GC
Cuts at: 0 355 538 720
Size: 355 183 182
Fragments arranged by size:
355 183 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 298 720
Size: 244 54 422
Fragments arranged by size:
422 244 54
BsaAI yAC'GTr
Cuts at: 0 594 720
Size: 594 126
BsaHI Gr'CG_yC
Cuts at: 0 400 524 720
Size: 400 124 196
Fragments arranged by size:
400 196 124
BseYI C'CCAG_C
Cuts at: 0 197 547 720
Size: 197 350 173
Fragments arranged by size:
350 197 173
BsiHKAI G_wGCw'C
Cuts at: 0 215 720
Size: 215 505
Bsp1286I G_dGCh'C
Cuts at: 0 215 467 602 720
Size: 215 252 135 118
Fragments arranged by size:
252 215 135 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 720
Size: 263 457
BsrBI CCG'CTC
Cuts at: 0 312 720
Size: 312 408
BsrFI r'CCGG_y
Cuts at: 0 390 481 720
Size: 390 91 239
Fragments arranged by size:
390 239 91
BssHII G'CGCG_C
Cuts at: 0 359 720
Size: 359 361
BtgI C'CryG_G
Cuts at: 0 332 720
Size: 332 388
ClaI AT'CG_AT
Cuts at: 0 173 720
Size: 173 547
DrdI GACnn_nn'nnGTC
Cuts at: 0 274 720
Size: 274 446
EaeI y'GGCC_r
Cuts at: 0 163 713 720
Size: 163 550 7
Fragments arranged by size:
550 163 7
EarI CTCTTCn'nnn_
Cuts at: 0 664 720
Size: 664 56
EcoO109I rG'GnC_Cy
Cuts at: 0 338 720
Size: 338 382
HaeII r_GCGC'y
Cuts at: 0 403 443 488 527 720
Size: 403 40 45 39 193
Fragments arranged by size:
403 193 45 40 39
HindIII A'AGCT_T
Cuts at: 0 139 720
Size: 139 581
KasI G'GCGC_C
Cuts at: 0 399 523 720
Size: 399 124 197
Fragments arranged by size:
399 197 124
MluI A'CGCG_T
Cuts at: 0 497 720
Size: 497 223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 720
Size: 368 352
MspA1I CmG'CkG
Cuts at: 0 66 542 720
Size: 66 476 178
Fragments arranged by size:
476 178 66
NaeI GCC'GGC
Cuts at: 0 392 720
Size: 392 328
NarI GG'CG_CC
Cuts at: 0 400 524 720
Size: 400 124 196
Fragments arranged by size:
400 196 124
NgoMIV G'CCGG_C
Cuts at: 0 390 720
Size: 390 330
PasI CC'CwG_GG
Cuts at: 0 507 720
Size: 507 213
PflMI CCAn_nnn'nTGG
Cuts at: 0 453 474 720
Size: 453 21 246
Fragments arranged by size:
453 246 21
PspXI vC'TCGA_Gb
Cuts at: 0 435 720
Size: 435 285
PstI C_TGCA'G
Cuts at: 0 638 720
Size: 638 82
PvuII CAG'CTG
Cuts at: 0 542 720
Size: 542 178
SfcI C'TryA_G
Cuts at: 0 634 720
Size: 634 86
SfoI GGC'GCC
Cuts at: 0 401 525 720
Size: 401 124 195
Fragments arranged by size:
401 195 124
SmaI CCC'GGG
Cuts at: 0 58 376 720
Size: 58 318 344
Fragments arranged by size:
344 318 58
SmlI C'TyrA_G
Cuts at: 0 259 313 435 720
Size: 259 54 122 285
Fragments arranged by size:
285 259 122 54
SrfI GCCC'GGGC
Cuts at: 0 376 720
Size: 376 344
StyI C'CwwG_G
Cuts at: 0 123 473 720
Size: 123 350 247
Fragments arranged by size:
350 247 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 720
Size: 450 270
XhoI C'TCGA_G
Cuts at: 0 435 720
Size: 435 285
XmaI C'CCGG_G
Cuts at: 0 56 374 720
Size: 56 318 346
Fragments arranged by size:
346 318 56
XmnI GAAnn'nnTTC
Cuts at: 0 40 720
Size: 40 680
Enzymes that do cut:
AflIII AvaI AvrII BanI BbeI BbsI BbvCI Bme1580I
Bpu10I BpuEI BsaAI BsaHI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BtgI ClaI DrdI EaeI EarI
EcoO109I HaeII HindIII KasI MluI MmeI MspA1I NaeI
NarI NgoMIV PasI PflMI PspXI PstI PvuII SfcI
SfoI SmaI SmlI SrfI StyI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI BaeI BamHI BanII BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI BpmI BsaI BsaBI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI CspCI DraI DraIII EagI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HpaI KpnI MfeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI ZraI